Nav: Home

New nuclear RNA retention activity discovered

May 16, 2018

Gene expression involves mRNA transport from its place of synthesis to the cytoplasm where protein translation occurs. However, many non-coding RNA species do not follow this flow and new data now demonstrate how cells prevent the unwanted export of RNA and instead ensure nuclear degradation.

Mammalian genomes produce a large repertoire of RNAs, which experience very different fates. Protein-coding mRNAs serve as a prototypic example of transcripts that are rapidly processed and exported from nucleus to the cytoplasm to translate protein. However, several RNAs, many of which are non-coding, are rapidly degraded in the nucleus by the highly conserved RNA degradation machine, the RNA exosome.

In recent work, postdoctoral researcher Toomas Silla from Torben Heick Jensen's laboratory at the Department of Molecular Biology and Genetics discovered that abolished RNA exosome function causes the accumulation of polyadenylated (pA+) RNAs in distinct nuclear foci (coined as pA+ foci), indicating that these accumulated RNAs are prevented from entering the cytoplasm. This hinted that so-called exosome adaptors, which are required for RNA exosome loading onto its substrates, might dually serve as RNA nuclear retention factors and counteract export activity.

Previously, the Torben Heick Jensen laboratory established the 'Nuclear EXosome Targeting (NEXT)' complex and 'PolyA eXosome Targeting (PAXT)' connection to be adaptor complexes that link the RNA exosome to short unprocessed or to longer polyadenylated RNAs, respectively (Lubas et al. Mol Cell 2011; Meola et al. Mol Cell 2016). Interestingly, co-localization analysis revealed, that pA+ foci are particularly enriched for PAXT proteins such as MTR4, ZFC3H1 and PABPN1. Among these factors, ZFC3H1 appeared to be critical for establishing the pA+ RNA foci; in ZFC3H1 depleted cells pA+ RNA foci are not formed, and exosome targets that would normally be degraded in the nucleus, are exported to the cytoplasm. These transcripts now enter into the RNA export pathway, demonstrating that ZFC3H1 counteracts RNA export activity to ensure the nuclear retention of exosome targets before their decay.

Transcriptome-wide characterization of pA+ RNA foci contents, revealed surprisingly many full-length and spliced mRNAs. Thus, in addition to bona fide RNA exosome targets, mRNAs may also be subjects to nuclear decay. Although most mRNAs are quickly exported after their production, these new results therefore suggest that mRNAs can be cut out of the export pathway and degraded in the nucleus instead. Future research will be focused on determining how sorting between productive export and retention/decay is orchestrated and which additional factors contribute to this process.
-end-
The scientific article was published in the internationally recognised journal Cell Reports.

"The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts" by Toomas Silla, Evdoxia Karadoulama, Dawid M?kosa, Michal Lubas, Torben Heick Jensen.

For further information, please contact

Professor Torben Heick Jensen

Department of Molecular Biology and Genetics

Aarhus University, Denmark

thj@mbg.au.dk - Mobile: +45 60202705

Aarhus University

Related Protein Articles:

Hi-res view of protein complex shows how it breaks up protein tangles
A new, high-resolution view of the structure of Hsp104 (heat shock protein 104), a natural yeast protein nanomachine with six subunits, may show news ways to dismantle harmful protein clumps in disease.
Breaking the protein-DNA bond
A new Northwestern University study finds that unbound proteins in a cell break up protein-DNA bonds as they compete for the single-binding site.
FASEB Science Research Conference: Protein Kinases and Protein Phosphorylation
This conference focuses on the biology of protein kinases and phosphorylation signaling.
Largest resource of human protein-protein interactions can help interpret genomic data
An international research team has developed the largest database of protein-to-protein interaction networks, a resource that can illuminate how numerous disease-associated genes contribute to disease development and progression.
STAT2: Much more than an antiviral protein
A protein known for guarding against viral infections leads a double life, new research shows, and can interfere with cell growth and the defense against parasites.
A protein makes the difference
It is well-established knowledge that blood vessels foster the growth of tumors.
Nuclear protein causes neuroblastoma to become more aggressive
Aggressive forms of neuroblastoma contain a specific protein in their cells' nuclei that is not found in the nuclei of more benign forms of the cancer, and the discovery, made through research from the University of Rochester Medical Center, could lead to new forms of targeted therapy.
How a protein could become the next big sweetener
High-fructose corn syrup and sugar are on the outs with calorie-wary consumers.
High animal protein intake associated with higher, plant protein with lower mortality rate
The largest study to examine the effects of different sources of dietary protein found that a high intake of proteins from animal sources -- particularly processed and unprocessed red meats -- was associated with a higher mortality rate, while a high intake of protein from plant sources was associated with a lower risk of death.
Protein in, ammonia out
A recent study has compiled and analyzed data from 25 previous studies.

Related Protein Reading:

Protein Power: The High-Protein/Low Carbohydrate Way to Lose Weight, Feel Fit, and Boost Your Health-in Just Weeks!
by Michael R. Eades (Author), Mary Dan Eades (Author)

New York Times Bestseller - An effective, medically sound diet that lets you eat bacon, eggs, steak, even cheese? It's true!  Lose fat.  Feel fit.  Stop craving.  Without counting fat grams and without giving up the foods you love. Includes recipes for healthy meals to lose weight.

Based on cutting-edge research, this revolutionary and deliciously satisfying plan has already helped thousands of patients lose weight and achieve other lifesaving health benefits, including lower cholesterol and blood pressure readings and an improvement or reversal of common... View Details


Clean Protein: The Revolution that Will Reshape Your Body, Boost Your Energy—and Save Our Planet
by Kathy Freston (Author), Bruce Friedrich (Author)

Join the CLEAN PROTEIN revolution and lose weight, feel stronger, and live longer.

Food and wellness experts Kathy Freston and Bruce Friedrich have spent years researching the future of protein. They've talked to the food pioneers and the nutrition scientists, and now they've distilled what they've learned into a strength-building plan poised to reshape your body and change your world.

Complete with delicious recipes and a detailed guide to food planning, Clean Protein explains everything you need to know in order to get lean, gain energy, and stay mentally sharp.... View Details


DIY Protein Bars Cookbook [2nd Edition]: Easy, Healthy, Homemade No-Bake Treats That Taste Like Dessert, But Just Happen To Be Packed With Protein!
by Jessica Stier (Author)

Welcome to the DIY Protein Bars Cookbook [2nd Edition]: a collection of 48 easy, healthy, no-bake treats that just happen to be packed with protein!

This hot-selling cookbook has been revamped with an all new, restyled interior design, upgraded photography, and updated recipes based on customer feedback and to streamline the bar-making process. Enjoy!

All of the recipes are (or can easily be made) gluten-free, dairy-free, soy-free, and vegan, but you’d never know it. These protein bars are so tasty, addicting, and good for you, you’ll... View Details


The Bariatric Foodie Guide to Perfect Protein Shakes (Volume 1)
by Ms Nikki L Massie (Author)

Life can be stressful. Your protein shake shouldn't be! Dozens of crave-worthy protein drink recipes tips & tricks from popular weight-loss surgery blog, Bariatric Foodie! View Details


Proteins: Structure and Function
by David Whitford (Author)

Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and... View Details


Proteins: Concepts in Biochemistry
by Paulo Almeida (Author)

Proteins: Concepts in Biochemistry teaches the biochemical concepts underlying protein structure, evolution, stability, folding, and enzyme kinetics, and explains how interactions in macromolecular structures determine protein function. Intended for a one-semester course in biochemistry or biophysical chemistry with a focus on proteins, this textbook emphasizes the logic underlying biophysical chemical principles.

Problems throughout the book encourage statistical and quantitative thinking. The text is ideal for senior undergraduates, first year graduate students, and... View Details


The Protein-Packed Breakfast Club: Easy High Protein Recipes with 300 Calories or Less to Help You Lose Weight and Boost Metabolism
by Lauren Harris-Pincus MS RDN (Author)

Whether for weight loss, managing prediabetes or Type II diabetes, or a healthy, fit lifestyle, The Protein-Packed Breakfast Club is filled with delicious, easy to make recipes containing 300 calories or less and packed with a minimum of 20 grams of protein. Power up your morning with protein! You’ll find recipes featuring dairy, protein powders, nuts, seeds, eggs and ancient grains including hot trends like overnight oats, smoothie bowls and mug cakes. Discover healthier versions of classics like pancakes and French toast. Many recipes are also vegetarian and gluten free. In a hurry in the... View Details


Proteins (Explore the molecules of life)
by Tali Lavy (Author), Ofir Corcos (Illustrator)

The human body relies on proteins for many of its most important processes. When we contract our muscles, we use proteins. We need proteins to fight disease and help blood cells transport oxygen. Proteins are the building blocks of life, and what’s more, they’re fascinating.

Written by structural biologist Tali Lavy, this book is a playfully illustrated exploration of proteins and their importance in human biology. Children will learn how proteins are composed of amino acids, how DNA encodes proteins, and how proteins affect life at a molecular level. Entertaining drawings of... View Details


Protein Ninja: Power through Your Day with 100 Hearty Plant-Based Recipes that Pack a Protein Punch
by Terry Hope Romero (Author)


I wanted protein recipes other than that mashing a vanilla-flavored powder with almond butter and flax seeds and calling it a day. I wanted something that really made me feel as if I was cooking. And yes, even good old-fashioned baking! Adding pure, unflavored, but wholesome plant-based protein powders to recipes brings out my inner foodie alchemist. I heard the call of the protein ninja.

Whether you're vegan, vegetarian, or eat-everything-you-can-get-your-hands-on, a weeknight home chef, everyday athlete, or just a busy person looking wholesome, protein-rich snacks and... View Details


Proteinaholic: How Our Obsession with Meat Is Killing Us and What We Can Do About It
by Garth Davis M.D. (Author), Howard Jacobson (Author)

An acclaimed surgeon specializing in weight loss delivers a paradigm-shifting examination of the diet and health industry’s focus on protein, explaining why it is detrimental to our health, and can prevent us from losing weight.

Whether you are seeing a doctor, nutritionist, or a trainer, all of them advise to eat more protein. Foods, drinks, and supplements are loaded with extra protein. Many people use protein for weight control, to gain or lose pounds, while others believe it gives them more energy and is essential for a longer, healthier life. Now, Dr. Garth Davis, an expert in... View Details

Best Science Podcasts 2018

We have hand picked the best science podcasts for 2018. Sit back and enjoy new science podcasts updated daily from your favorite science news services and scientists.
Now Playing: TED Radio Hour

Inspire To Action
What motivates us to take up a cause, follow a leader, or create change? This hour, TED speakers explore stories of inspirational leadership, and what makes some movements more successful than others. Guests include high school history teacher Diane Wolk-Rogers, writer and behavioral researcher Simon Sinek, 2016 Icelandic presidential candidate Halla Tómasdóttir, professor of leadership Jochen Menges, and writer and activist Naomi Klein.
Now Playing: Science for the People

#474 Appearance Matters
This week we talk about appearance, bodies, and body image. Why does what we look like affect our headspace so much? And how do we even begin to research a topic as personal and subjective as body image? To try and find out, we speak with some of the researchers at the Centre for Appearance Research (CAR) at the University of the West of England in Bristol. Psychology Professor Phillippa Diedrichs walks us through body image research, what we know so far, and how we know what we know. Professor of Appearance and Health Psychology Diana Harcourt talks about visible...