News tips from the Journal of Neuroscience

May 20, 2008

1. Compound Fusion at Ribbon Synapses
Gary Matthews and Peter Sterling

How ribbon synapses in photoreceptors and retinal bipolar cells can release vesicles extremely rapidly has long been a mystery. Diffusion of undocked vesicles to the plasma membrane is too slow to account for the speed of release. Matthews and Sterling now provide electron-microscopic evidence that compound fusion--in which vesicles fuse to each other before fusing with the plasma membrane--occurs at ribbon synapses of goldfish bipolar cells. Stimulated ribbon synapses had significantly fewer vesicles tethered to the ribbon than those in which exocytosis was blocked. Although most vesicles had similar sizes in stimulated and unstimulated synapses, vesicles up to three times larger appeared after stimulation. The largest of these occurred at the base of the ribbon, adjacent to the plasma membrane, whereas normal-sized vesicles predominated at the top of the ribbon. Invaginations of the plasma membrane near ribbon synapses also appeared after stimulation, and like vesicles, these were tethered to the ribbon.

2. Depolarizing GABA Currents, NMDA Receptors, and Synaptogenesis
Doris D. Wang and Arnold R. Kriegstein

In the developing neocortex, GABAergic synapses form before glutamatergic synapses, but GABA is depolarizing due to elevated internal chloride. GABA-mediated depolarization regulates subsequent development of both GABAergic and glutamatergic synapses. To elucidate how GABAergic depolarization affects synaptic development, Wang and Kriegstein made GABA hyperpolarizing in embryonic mice by blocking the chloride transporter NKCC1 in utero. As shown previously, this disrupted postnatal AMPA transmission and reduced dendrite length and branching. At 1-3 weeks postnatal, the frequency of spontaneous GABAergic and AMPAergic postsynaptic currents was reduced in Nkcc1-knockdown mice compared to controls. Furthermore, dendritic spine density decreased and spine length increased in knockdown mice, suggesting fewer glutamatergic synapses were present. Synapse development in mutant mice was rescued by cotransfection of a voltage-independent NMDA receptor (NMDAR), and knockdown of an NMDAR subunit replicated the effects of NKCC1 knockdown. These data suggest that depolarizing GABA currents enable NMDAR activation, which, in turn, is necessary for AMPA synapse formation.

3. Opioid Attenuation of Human Fear Conditioning
Falk Eippert, Ulrike Bingel, Eszter Schoell, Juliana Yacubian, and Christian Büchel

When pain is paired with an innocuous stimulus, animals learn to fear the innocuous stimulus. But pain also triggers release of endogenous opioids, which dampen pain--does this affect fear conditioning" It has been hypothesized that opioids provide negative feedback that attenuates painful stimuli and thereby tempers fear conditioning. If so, opioid antagonists should enhance conditioning. This week, Eippert et al. provide evidence supporting this hypothesis in humans. The authors monitored fear conditioning using a reaction time task, functional magnetic resonance imaging, skin conductance, and subjective pain ratings in subjects treated with an opioid antagonist or saline. Antagonist-treated subjects demonstrated stronger conditioning (faster reaction times), while controls showed more habituation in brain regions involved in pain processing. Furthermore, brain regions involved in descending pain control (rostral anterior cingulate cortex, amygdala, and periaqueductal gray) were deactivated when the conditioned stimulus was presented to controls, suggesting inhibition of these regions may be responsible for attenuating conditioning.

4. Interconnections of Medium Spiny Neurons
Stefano Taverna, Ema Ilijic, and D. James Surmeier

The two main types of medium spiny neurons (MSNs) in the striatum--those that project directly to the substantia nigra and those that project indirectly via the globus pallidus-- have opposing effects on thalamic outputs and behavior, but are known to be interconnected. This week, Taverna et al. present a systematic analysis of these GABAergic interconnections, revealing differences between MSN types. Recording from pairs of neurons, they found that when neurons were connected, it was always unidirectionally: no pairs were reciprocally connected. In addition, MSNs that expressed D1 dopamine receptors (D1 MSNs, mostly striatonigral) synapsed primarily onto other D1 MSNs, whereas those that expressed D2 receptors (D2 MSNs, mainly striatopallidal) synapsed onto both types. Furthermore, when the presynaptic cell was a D2 MSN, PSPs were larger than when the presynaptic cell was a D1 MSN, regardless of the postsynaptic cell type. Depletion of dopamine (a model of Parkinson's disease) greatly reduced collateral connections of all types.
Please click here for the current table of contents.

Society for Neuroscience

Related Neurons Articles from Brightsurf:

Paying attention to the neurons behind our alertness
The neurons of layer 6 - the deepest layer of the cortex - were examined by researchers from the Okinawa Institute of Science and Technology Graduate University to uncover how they react to sensory stimulation in different behavioral states.

Trying to listen to the signal from neurons
Toyohashi University of Technology has developed a coaxial cable-inspired needle-electrode.

A mechanical way to stimulate neurons
Magnetic nanodiscs can be activated by an external magnetic field, providing a research tool for studying neural responses.

Extraordinary regeneration of neurons in zebrafish
Biologists from the University of Bayreuth have discovered a uniquely rapid form of regeneration in injured neurons and their function in the central nervous system of zebrafish.

Dopamine neurons mull over your options
Researchers at the University of Tsukuba have found that dopamine neurons in the brain can represent the decision-making process when making economic choices.

Neurons thrive even when malnourished
When animal, insect or human embryos grow in a malnourished environment, their developing nervous systems get first pick of any available nutrients so that new neurons can be made.

The first 3D map of the heart's neurons
An interdisciplinary research team establishes a new technological pipeline to build a 3D map of the neurons in the heart, revealing foundational insight into their role in heart attacks and other cardiac conditions.

Mapping the neurons of the rat heart in 3D
A team of researchers has developed a virtual 3D heart, digitally showcasing the heart's unique network of neurons for the first time.

How to put neurons into cages
Football-shaped microscale cages have been created using special laser technologies.

A molecule that directs neurons
A research team coordinated by the University of Trento studied a mass of brain cells, the habenula, linked to disorders like autism, schizophrenia and depression.

Read More: Neurons News and Neurons Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to