Biomarkers for interstitial cystitis identified, could lead to the first test

May 21, 2005

SAN ANTONIO, May 21 - University of Pittsburgh researchers have isolated two biomarkers for interstitial cystitis (IC), a chronic and painful pelvic disease for which there currently is no test. The discovery of these biomarkers could lead to a definitive test for IC and have the potential to lead to new therapies. Results of two studies are being presented today at the annual meeting of the American Urological Association (AUA) in San Antonio, and are published in abstracts 69 and 80 of the AUA proceedings.

"IC is a frustrating disease for patients because, to this point, there is no accurate way of diagnosing the condition. Patients undergo a variety of tests to rule out other diseases, all while experiencing significant pain and discomfort. Only after these tests come back negative, can a doctor make the diagnosis of IC," said Michael Chancellor, M.D., professor, department of urology, University of Pittsburgh School of Medicine.

"Finding a marker for IC can not only make developing an early test for IC possible, but it can lead to new targeted molecular therapies for the condition," said Fernando de Miguel, Ph.D., assistant professor of urology at Pitt's School of Medicine.

In the first study, titled "Identification of Nuclear Proteins in the Chronic Cystitic Rat Model" (abstract 69), researchers used a proteomic approach to identify specific markers related to IC. By comparing protein expression in the bladder tissue of two animal models of IC to expression in the tissue of a normal animal, the researchers found three nuclear proteins that were unique to the animals with IC. Using protein mass fingerprinting, the proteins were identified as transgelin (SM-22), ras suppressor protein (RSU-1) and GAPDH.

In the second study, titled "Time-point study of the Regulation of Nuclear Protein SM-22 (Transgelin) in the Rat Cystitis Model" (abstract 80), the researchers expanded their investigation into the expression of SM-22 in both normal and IC-model bladders. The bladders were instilled with hydrochloric acid; tissue was analyzed at one, four, seven, 13 and 28 days after instillation. After day one and day four, there was a noticeable down-regulation of SM-22 in the IC-model bladders; by day 28, there was a reduction by 31 percent of the SM-22 in the diseased models.

The early down-regulation of SM-22, evident as early as day one, shows that the absence of SM-22 can potentially be used as an early diagnostic marker for IC. The University of Pittsburgh researchers plan to conduct more research into SM-22 to determine the protein's functional role, which could lead the way to future molecular-targeted therapies.

According to the National Institute of Diabetes and Digestive and Kidney Diseases, 700,000 Americans have IC; 90 percent are women. IC is one of the chronic pelvic pain disorders, defined by recurring discomfort or pain in the bladder and surrounding pelvic region. Symptoms vary and can include any combination of mild to severe pain, pressure and tenderness in the bladder and pelvic area; and an urgent and/or frequent need to urinate. In IC, the bladder wall may become scarred or irritated, and pinpoint bleeding may appear on the bladder wall.

Also contributing to this research were Thu-Suong Van Le, Uukio Hayashi, Shachi Tyagi and Naoki Yoshimura, all from the University of Pittsburgh.
-end-
Research was funded through grants from the National Institute of Diabetes and Digestive and Kidney Diseases and the Fishbein Family CURE-IC.

University of Pittsburgh Medical Center

Related Biomarkers Articles from Brightsurf:

Urgent need for blood-based biomarkers to diagnosis concussion
There is an urgent need for objective markers for diagnosing concussion, or mild traumatic brain injury.

Engineered bacteria churn out cancer biomarkers
A Cornell lab has created these very tools by commandeering simple, single-celled microorganisms - namely E. coli bacteria - and engineering them to explore the complex process of glycosylation and the functional role that protein-linked glycans play in health and disease.

Exercise induces secretion of biomarkers into sweat
The aim was to reveal the potential of microRNAs in sweat extracellular vesicles in monitoring exercise performance.

Phosphoprotein biomarkers to guide cancer therapy are identified
Researchers led by James Bibb, Ph.D., professor of surgery at the University of Alabama at Birmingham, suggest using a broader lens of post-translational modification analysis to identify new biomarkers of cancer drivers that may allow a much more precise prediction of patient responses to treatments.

Exhaled biomarkers can reveal lung disease
Using specialized nanoparticles, MIT engineers have developed a way to diagnose pneumonia or other lung diseases by analyzing the breath exhaled by the patient.

Race-specific lupus nephritis biomarkers
A University of Houston biomedical researcher has discovered a difference in urinary biomarker proteins of lupus nephritis in patients according to race.

Semen miRNAs could be non-invasive biomarkers for prostate cancer
Researchers of the Human Molecular Genetics group at the Bellvitge Biomedical Research Institute (IDIBELL), led by Dr.

Scientists have found longevity biomarkers
An international group of scientists studied the effects of 17 different lifespan-extending interventions on gene activity in mice and discovered genetic biomarkers of longevity.

After concussion, biomarkers in the blood may help predict recovery time
A study of high school and college football players suggests that biomarkers in the blood may have potential use in identifying which players are more likely to need a longer recovery time after concussion, according to a study published in the July 3, 2019, online issue of Neurology, the medical journal of the American Academy of Neurology.

3D-printed device detects biomarkers of preterm birth
Preterm birth (PTB) -- defined as birth before the 37th week of gestation -- is the leading complication of pregnancy.

Read More: Biomarkers News and Biomarkers Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.