Nav: Home

Scientists crack origin of the Persian walnut

June 04, 2019

Prized worldwide for its high-quality wood and rich flavor of delicious nuts, the Persian walnut (Juglans regia) is an important economic crop. The Persian walnut is one of 22 species in the genus Juglans, which includes black and white walnuts and butternuts, grown across Europe, the Americas and Asia.

China leads world production, followed by California, Turkey and Iran. But until now, the evolution of walnuts has been unknown. Walnuts have a rich fossil record, which suggest an origin of walnuts and initial divergence into black walnuts and butternuts (white walnuts) in North America, some 35-45 million years ago. With this high age, both walnut lineages would have had ample opportunity to migrate into the Old World via the Bering and North Atlantic land bridges, yet only butternuts have been detected in the fossil records of Europe and Asia, and no ancient fossils of the Persian walnut are known.

Using genomic data analyzed with phylogenetic and population genetic approaches, researchers have now cracked this mystery, showing that the Persian walnut is the result of hybridization between two long-extinct species around 3.45 million years ago.

Past analyses by the team based on some 2900 single-copy nuclear genes from 19 species of walnuts were unable to sort out the relationships between North American, Asian, and Persian walnut species. However, they excluded incomplete lineage sorting as the cause of the phylogenetic uncertainty. "This led us to speculate that ancient hybridization might be involved in the origin of the Persian walnut and the American butternut," said Da-Yong Zhang, a population geneticist who is one of the senior authors.

To test their hybridization hypothesis, rather than look at individual genes, this time, they used whole-genome sequencing from 80 individual trees that represented 19 of the 22 species of Juglans.

"We applied a battery of genome-wide methods for hybridization detection and to test speciation models to infer the time of origin of the Persian walnut," said the Wei-Ning Bai, another senior author. "In the end, we characterized the genetic composition of the genomes of not only the Persian walnut, but also the Iron walnut, J. sigillata, and the American butternut, J. cinerea." The authors paint a picture, based on walnut fruit fossils and their genomic evidence, that walnuts "by the late Oligocene must have expanded from America to Europe and then spread to Asia and further expanded into Europe, where the black walnut and butternut lineages came into contact and hybridized some 3.45 Ma ago. While walnuts diversified in Asia and the New World, they were lost from Europe during the Pleistocene climate oscillations, with eventually only the newly formed hybrid lineage that gave rise to J. regia surviving."

"All of our genome-wide analyses converged to provide unambiguous evidence of hybridization at the roots of both the American butternut (J. cinerea) and the Persian walnut (J. regia/J. sigillata)," said the authors. "Our results further resolve the controversy concerning the origin of the American butternut, J. cinerea, which turns out to result from massive nuclear gene introgression involving an American black walnut through pollen swamping by an immigrating Asian butternut."

"The genomic mosaicism of both the Persian walnut (incl. J. sigillata) and the American butternut J. cinerea provides evidence for the importance of hybridization throughout the evolution of walnuts," said the authors.

Molecular Biology and Evolution (Oxford University Press)

Related Evolution Articles:

Chemical evolution -- One-pot wonder
Before life, there was RNA: Scientists at Ludwig-Maximilians-Universitaet (LMU) in Munich show how the four different letters of this genetic alphabet could be created from simple precursor molecules on early Earth -- under the same environmental conditions.
Catching evolution in the act
Researchers have produced some of the first evidence that shows that artificial selection and natural selection act on the same genes, a hypothesis predicted by Charles Darwin in 1859.
Guppies teach us why evolution happens
New study on guppies shows that animals evolve in response the the environment they create in the absence of predators, rather than in response to the risk of being eaten.
Undercover evolution
Our individuality is encrypted in our DNA, but it is deeper than expected.
Evolution designed by parasites
In 'Invisible Designers: Brain Evolution Through the Lens of Parasite Manipulation,' published in the September 2019 issue of The Quarterly Review of Biology, Marco Del Giudice explores an overlooked aspect of the relationship between parasites and their hosts by systematically discussing the ways in which parasitic behavior manipulation may encourage the evolution of mechanisms in the host's nervous and endocrine systems.
More Evolution News and Evolution Current Events

Best Science Podcasts 2019

We have hand picked the best science podcasts for 2019. Sit back and enjoy new science podcasts updated daily from your favorite science news services and scientists.
Now Playing: TED Radio Hour

Erasing The Stigma
Many of us either cope with mental illness or know someone who does. But we still have a hard time talking about it. This hour, TED speakers explore ways to push past — and even erase — the stigma. Guests include musician and comedian Jordan Raskopoulos, neuroscientist and psychiatrist Thomas Insel, psychiatrist Dixon Chibanda, anxiety and depression researcher Olivia Remes, and entrepreneur Sangu Delle.
Now Playing: Science for the People

#537 Science Journalism, Hold the Hype
Everyone's seen a piece of science getting over-exaggerated in the media. Most people would be quick to blame journalists and big media for getting in wrong. In many cases, you'd be right. But there's other sources of hype in science journalism. and one of them can be found in the humble, and little-known press release. We're talking with Chris Chambers about doing science about science journalism, and where the hype creeps in. Related links: The association between exaggeration in health related science news and academic press releases: retrospective observational study Claims of causality in health news: a randomised trial This...