Nav: Home

Genome's gyrations fit right into Rice University model

July 09, 2018

The nuclei of cells are never static, even when the chromosomes they contain appear to be at rest. Theorists at Rice University have detailed the combination of forces that drive their constant motion.

An extension of their landmark energy landscape model for proteins helped researchers at Rice's Center for Theoretical Biological Physics form a theory that unifies the structural and dynamic aspects of the genome during interphase, the time before reproduction when nuclear DNA conducts the symphony of activity that keeps the cell humming.

Scientists have long been able to watch cells go through their cycle from interphase to mitosis and back, but it has been difficult to understand what's happening during each phase. In the new study, the Rice researchers determined that the energy landscape model they developed to analyze the spatial folding of chromosomes could also account for genome dynamics that experimentalists have recently observed during interphase.

The open access paper published today in the Proceedings of the National Academy of Sciences by physicists José Onuchic and Peter Wolynes and postdoctoral researchers Michele Di Pierro and Davit Potoyan draws upon the lab's long experience in modeling how proteins fold.

Those models rely on coarse-grained algorithms in which a subset of amino acids in a chain - either a protein or DNA - represents the entire chain. The inherent energies of the individual acids determine how the chain will fold and how likely they are to associate with other beads along the chain.

Over the past four years, the researchers have refined their algorithms into their Minimal Chromatin Model, which simulates the energy landscape of DNA as it divides, condenses into a tight blob during interphase and then blossoms into the familiar X- and Y-shaped chromosomes in preparation for mitosis.

The model shows that both random diffusion driven by Brownian motion and more directed movements triggered by molecular motors help DNA wriggle in its nuclear sphere as it continually reorganizes itself.

"At the molecular scale, the ordinary thermal motion of particles is enough to move them around and get them out of jams where they're too strongly bound to other particles," Wolynes said. "Typically, they go to the state that's the most thermodynamically stable. For protein folding, these thermal motions are paramount, but in the macroscopic world, they are completely inadequate.

"The problem at the scale of the chromosome is that it's much smaller than macroscopic things we see around us but much larger than a molecule. So which rules are we supposed to apply?"

The answer turned out to be both.

Wolynes noted that experiments had showed diffusion-like motion within the chromosome, but also evidence that ATP-driven molecular motors were helping direct things. Experiments by other labs that stopped the motors also stopped some of the changes in chromosomal structure.

"We decided to see what happens if our model assumes the movements are equivalent to those at equilibrium but are being stirred about by motors that more or less do what the random thermal motions do, but more violently," he said. "That got us to something that was very much in agreement with experiments."

The simulated interactions between two chromosomes in a lymphoblastoid cell also revealed the presence of between 10 and 20 dynamically associated domains (DADs), blobs of compartmentalized chromatin -- segments of DNA and their associated epigenetic markers and other proteins -- that tended to move in a coherent way. The researchers argue that DADs arise from the microscale phase separation, like oil and water, of chromatin segments that have different biochemical properties.

"These DADs are a consequence of a competition between the tendency to phase-separate and the constraints that arise from the polymer nature of chromatin," Onuchic said. Unlike oil and water that fully separate, chromatin creates multiple domains that segregate by type, he said.

"We found locations in the chromosome that deviate from the diffusion law, as some of them diffuse faster than others," Wolynes said. "These jelly droplets are all different sizes. Some diffuse for a long time, some for a shorter time, and there's a threshold that defines these modes of motion."

He compared them to mayonnaise, in which the proteins in egg yolk form droplets that bind oil and water. "Phase separation in chromatin makes the chromosome into a kind of salad dressing in which droplets with different epigenetic marks move coherently," Wolynes said.

Di Pierro said the work should appeal to both biologists who study the structure of DNA and the biophysicists who study its movement. "We want our theory to bridge that gap between dynamics and structure," he said. "With a bit of physics and a bit of engineering, we can actually show that what looks weird from one point of view or the other all fits into the same picture."
-end-
The National Science Foundation and the Welch Foundation supported the research. Wolynes is the D.R. Bullard-Welch Foundation Professor of Science and a professor of chemistry, biochemistry and cell biology, physics and astronomy and materials science and nanoengineering. Onuchic is the Harry C. and Olga K. Wiess Chair of Physics and a professor of physics and astronomy, of chemistry and of biochemistry and cell biology.

Read the paper at http://www.pnas.org/cgi/doi/10.1073/pnas.1806297115

This news release can be found online at http://news.rice.edu/2018/07/09/genomes-gyrations-fit-right-into-rice-university-model/

Follow Rice News and Media Relations via Twitter @RiceUNews.

Video:

0702_CHROMOSOME%20VIDEO

https://youtu.be/a87Al3En9hU

CAPTION: A simulation of two chromosomes during interphase shows dynamically associated domains of chromatin moving coherently in 10-second intervals. The models developed at Rice University demonstrate that energy landscape theory can predict not only the form of DNA contained in a cell's nucleus during interphase but also its dynamic behavior. (Credit: Center for Theoretical Biological Physics/Rice University)

Related materials:

Chromosome-folding theory shows promise: http://news.rice.edu/2015/04/28/chromosome-folding-theory-shows-promise/

DNA in 'unbiased' model curls both ways: http://news.rice.edu/2016/06/14/dna-in-unbiased-model-curls-both-ways-2/

Rice biophysicists model genome mechanics: http://news.rice.edu/2016/09/26/rice-biophysicists-model-genome-mechanics-2/

Chromosome organization emerges from 1D patterns: http://news.rice.edu/2017/10/31/chromosome-organization-emerges-from-1-d-patterns-2/

Onuchic bio: https://en.wikipedia.org/wiki/José_Onuchic

Wolynes bio: https://chemistry.rice.edu/FacultyDetail.aspx?p=ACC7DC090095C11C

Center for Theoretical Biological Physics: https://ctbp.rice.edu

Rice Department of Physics and Astronomy: http://www.physics.rice.edu

Located on a 300-acre forested campus in Houston, Rice University is consistently ranked among the nation's top 20 universities by U.S. News & World Report. Rice has highly respected schools of Architecture, Business, Continuing Studies, Engineering, Humanities, Music, Natural Sciences and Social Sciences and is home to the Baker Institute for Public Policy. With 3,970 undergraduates and 2,934 graduate students, Rice's undergraduate student-to-faculty ratio is just under 6-to-1. Its residential college system builds close-knit communities and lifelong friendships, just one reason why Rice is ranked No. 1 for quality of life and for lots of race/class interaction and No. 2 for happiest students by the Princeton Review. Rice is also rated as a best value among private universities by Kiplinger's Personal Finance. To read "What they're saying about Rice," go to http://tinyurl.com/RiceUniversityoverview.

Rice University

Related Dna Articles:

Scientists now know what DNA's chaperone looks like
Researchers have discovered the structure of the FACT protein -- a mysterious protein central to the functioning of DNA.
In one direction or the other: That is how DNA is unwound
DNA is like a book, it needs to be opened to be read.
DNA is like everything else: it's not what you have, but how you use it
A new paradigm for reading out genetic information in DNA is described by Dr.
A new spin on DNA
For decades, researchers have chased ways to study biological machines.
From face to DNA: New method aims to improve match between DNA sample and face database
Predicting what someone's face looks like based on a DNA sample remains a hard nut to crack for science.
Self-healing DNA nanostructures
DNA assembled into nanostructures such as tubes and origami-inspired shapes could someday find applications ranging from DNA computers to nanomedicine.
DNA design that anyone can do
Researchers at MIT and Arizona State University have designed a computer program that allows users to translate any free-form drawing into a two-dimensional, nanoscale structure made of DNA.
DNA find
A Queensland University of Technology-led collaboration with University of Adelaide reveals that Australia's pint-sized banded hare-wallaby is the closest living relative of the giant short-faced kangaroos which roamed the continent for millions of years, but died out about 40,000 years ago.
DNA structure impacts rate and accuracy of DNA synthesis
DNA sequences with the potential to form unusual conformations, which are frequently associated with cancer and neurological diseases, can in fact slow down or speed up the DNA synthesis process and cause more or fewer sequencing errors.
Changes in mitochondrial DNA control how nuclear DNA mutations are expressed in cardiomyopathy
Differences in the DNA within the mitochondria, the energy-producing structures within cells, can determine the severity and progression of heart disease caused by a nuclear DNA mutation.
More Dna News and Dna Current Events

Top Science Podcasts

We have hand picked the top science podcasts of 2019.
Now Playing: TED Radio Hour

In & Out Of Love
We think of love as a mysterious, unknowable force. Something that happens to us. But what if we could control it? This hour, TED speakers on whether we can decide to fall in — and out of — love. Guests include writer Mandy Len Catron, biological anthropologist Helen Fisher, musician Dessa, One Love CEO Katie Hood, and psychologist Guy Winch.
Now Playing: Science for the People

#542 Climate Doomsday
Have you heard? Climate change. We did it. And it's bad. It's going to be worse. We are already suffering the effects of it in many ways. How should we TALK about the dangers we are facing, though? Should we get people good and scared? Or give them hope? Or both? Host Bethany Brookshire talks with David Wallace-Wells and Sheril Kirschenbaum to find out. This episode is hosted by Bethany Brookshire, science writer from Science News. Related links: Why Climate Disasters Might Not Boost Public Engagement on Climate Change on The New York Times by Andrew Revkin The other kind...
Now Playing: Radiolab

An Announcement from Radiolab