Nav: Home

Studies of all PIH proteins in zebrafish reveal distinct roles in axonemal dynein assembly

July 17, 2018

A research team led by Masahide Kikkawa, a professor of the University of Tokyo, established zebrafish mutants of all four PIH-protein genes and solved the structures of axonemal dyneins in mutant spermatozoa by cryo-electron tomography.

Construction of motile cilia/flagella requires cytoplasmic preassembly of axonemal dyneins before transport into cilia. Axonemal dyneins have various subtypes, but the roles of each dynein subtype and their assembly processes remain elusive in vertebrates. The PIH protein family, consisting of four members, has been implicated in the assembly of different dynein subtypes, although evidence for this idea is sparse. Here, we established zebrafish mutants of all four PIH-protein genes: pih1d1, pih1d2, ktu, and twister, and analyzed the structures of axonemal dyneins in mutant spermatozoa by cryo-electron tomography. Mutations caused the loss of specific dynein subtypes, which was correlated with abnormal sperm motility. We also found organ-specific compositions of dynein subtypes, which could explain the severe motility defects of mutant Kupffer's vesicle cilia. Our data demonstrate that all vertebrate PIH proteins are differently required for cilia/flagella motions and the assembly of axonemal dyneins, assigning specific dynein subtypes to each PIH protein.
-end-


Japan Science and Technology Agency

Related Cilia Articles:

Why the Galapagos cormorant lost its ability to fly
A new study points to a number of genes that may underlie the loss of flight in the Galapagos cormorant.
Cilia structure plays a major role in determining susceptibility to neural tube defects
Research published online in The FASEB Journal shows that the improper methylation of a protein called 'Septin2,' which regulates the structure of cilia, was associated with an increased risk of having a neural tube defect (NTD) and confirms that cilia are important factors in determining susceptibility of NTDs.
Transport of molecular motors into cilia
Molecular motors produce the force that powers the beat of sperm cell tails to generate movement toward the egg cell for fertilization.
FASEB Science Research Conference: The Biology of Cilia and Flagella
Research on the biology of the cilium has seen explosive growth as its essential roles in cell signaling and human disease are now well recognized.
IFT20 protein's role in helping cancer cells to invade
An international research team has discovered that the IFT20 protein helps some cancer cells to invade by facilitating the transportation of membranes and proteins within parts of the cell.
Scientists show how cells communicate
Primary cilia are antenna-like structures that are present on the surface of most cells in the human body.
Some cells need a 'haircut' before duplicating
Many of our cells are equipped with a hairlike 'antenna' that relays information about the external environment to the cell, and scientists have already discovered that the appearance and disappearance of these so-called primary cilia are synchronized with the process of cellular duplication, called mitosis
New book on Cilia from Cold Spring Harbor Laboratory Press
'Cilia', from CSHLPress, examines key aspects of ciliary biology -- from the molecular to the organismal level -- in normal physiology and disease.
Scientists find cause of facial widening defects
Widening across the forehead and nose occurs when loss of cilia at the surface of the cells disrupts internal signaling and causes two GLI proteins to stop repressing midfacial growth.
Faculty team awarded $1.25 million to study 'swimming cells'
They are the tiny motors present in many of the human body's most complex systems: cilia and flagella move liquids such as cerebrospinal fluid and mucus past the cell surface, and throughout the body.

Related Cilia Reading:

Best Science Podcasts 2019

We have hand picked the best science podcasts for 2019. Sit back and enjoy new science podcasts updated daily from your favorite science news services and scientists.
Now Playing: TED Radio Hour

Bias And Perception
How does bias distort our thinking, our listening, our beliefs... and even our search results? How can we fight it? This hour, TED speakers explore ideas about the unconscious biases that shape us. Guests include writer and broadcaster Yassmin Abdel-Magied, climatologist J. Marshall Shepherd, journalist Andreas Ekström, and experimental psychologist Tony Salvador.
Now Playing: Science for the People

#513 Dinosaur Tails
This week: dinosaurs! We're discussing dinosaur tails, bipedalism, paleontology public outreach, dinosaur MOOCs, and other neat dinosaur related things with Dr. Scott Persons from the University of Alberta, who is also the author of the book "Dinosaurs of the Alberta Badlands".