New nanoparticle combination therapy shows effective resuscitation for massive hemorrhage

July 30, 2019

Massive hemorrhage, or excessive bleeding, is a potentially preventable yet a major cause of death in trauma patients.

An immediate and well-balanced transfusion of blood components, such as platelets and red blood cells (RBCs) which, respectively form clots to stop bleeding and deliver oxygen to tissues and organs for proper function, becomes crucial for their survival.

Unfortunately, however, blood products may not always be in stock in case of an emergency, such as when a serious car accident involving many drivers and passengers occurs, because they cannot be preserved for long. For instance, a pack of RBCs can only be stored for 3 weeks at 2-6°C. This becomes only four days at room temperature for a platelet concentrate, and it must be constantly shaken in a shaker.

To act as substitutes for these components, a Japanese research team successfully resuscitated rabbits with coagulopathy, or an impaired ability to form blood clots, from severe hemorrhage shock by using two different nanoparticles; one with hemostatic potential and the other with an oxygen-carrying potential.

Shinji Takeoka of Waseda University said, "Our findings suggest not only that the nanoparticles we developed have excellent preservation property but also that the combination therapy using the hemostatic nanoparticles and the oxygen-carrying nanoparticles are comparable to conventional transfusion."

This study, published in Transfusion, is the first to report a combination therapy using two different types of functional nanoparticles.

"In this experiment, the research team penetrated the liver of rabbits with abnormally low levels of platelets to induce massive hemorrhage and administered the hemostatic nanoparticles developed in a previous study while blocking the bleeding site immediately after injury," Manabu Kinoshita of the National Defense Medical College Japan explains. "After five minutes, clotting was confirmed, and the oxygen-carrying nanoparticles were given to help the rabbits recover from anemia due to blood loss."

As a result, six out of the ten rabbits treated by this new combination therapy survived in comparison to the seven out of ten by conventional transfusion of platelet and RBC concentrates, showing similar hemorrhage control.

Hiromi Sakai of Nara Medical University said, "The hemostatic particles we developed can be stored for about one year at room temperature, with no shaking required. The same goes for oxygen-carrying nanoparticles. Also, the oxygen-carrying nanoparticles do not need any kind of blood typing or cross-match tests."

The team believes that the combination therapy using these nanoparticles will be effective for both in-hospital care and pre-hospital resuscitation.

Additionally, because blood supply is anticipated to decrease in countries with a low birthrate and an aging population such as Japan, the team hopes that the nanoparticles will be used for practical application in the future to save lives of patients who require massive transfusion in emergency trauma care.
The paper "Combination therapy using fibrinogen γ-chain peptide-coated, ADP-encapsulated liposomes and hemoglobin vesicles for trauma-induced massive hemorrhage in thrombocytopenic rabbits" appeared on July 1, 2019 in Transfusion (volume 59, issue 7), with DOI:

This work was supported in part by JSPS KAKENHI Grant Number 16K11435 and by Japan Agency for Medical Research and Development (AMED) Grant Number 17lk0201034h0003.

University news on this study

About Waseda University

Located in the heart of Tokyo, Waseda University is a leading private research university which has long been dedicated to academic excellence, innovative research and civic engagement at both the local and global levels since 1882. Today, the student body at Waseda is approximately 50,000, over 7,900 of whom are from overseas, hailing from 125 countries. To learn more about Waseda University, visit National Defense Medical College (NDMC)

Founded in 1973 as a medical academy under control of the Ministry of Defense Japan. The mission of NDMC is to educate and train future military officers who serve as medical doctors, nurse, and technical officers of the Self-Defense Forces. NDMC Hospital and NDMC Research Institute, which was specially established in order to study disaster medicines, provide an extraordinarily rich environment for scientific discovery and professional growth.

About Nara Medical University (NMU)

Located in Kashihara, Nara, the ancient capital of Japan at around 7th century, NMU has opened as a prefectural University since 1948. As one of unique activities, we have been promoting the concept of medicine-based town (MBT), which aims to contribute to future society by medical approach, in order to utilize our knowledge and skills not only for medical practice but also for all things related to industrial creation and regional revitalization.

Waseda University

Related Science Articles from Brightsurf:

75 science societies urge the education department to base Title IX sexual harassment regulations on evidence and science
The American Educational Research Association (AERA) and the American Association for the Advancement of Science (AAAS) today led 75 scientific societies in submitting comments on the US Department of Education's proposed changes to Title IX regulations.

Science/Science Careers' survey ranks top biotech, biopharma, and pharma employers
The Science and Science Careers' 2018 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.

Science in the palm of your hand: How citizen science transforms passive learners
Citizen science projects can engage even children who previously were not interested in science.

Applied science may yield more translational research publications than basic science
While translational research can happen at any stage of the research process, a recent investigation of behavioral and social science research awards granted by the NIH between 2008 and 2014 revealed that applied science yielded a higher volume of translational research publications than basic science, according to a study published May 9, 2018 in the open-access journal PLOS ONE by Xueying Han from the Science and Technology Policy Institute, USA, and colleagues.

Prominent academics, including Salk's Thomas Albright, call for more science in forensic science
Six scientists who recently served on the National Commission on Forensic Science are calling on the scientific community at large to advocate for increased research and financial support of forensic science as well as the introduction of empirical testing requirements to ensure the validity of outcomes.

World Science Forum 2017 Jordan issues Science for Peace Declaration
On behalf of the coordinating organizations responsible for delivering the World Science Forum Jordan, the concluding Science for Peace Declaration issued at the Dead Sea represents a global call for action to science and society to build a future that promises greater equality, security and opportunity for all, and in which science plays an increasingly prominent role as an enabler of fair and sustainable development.

PETA science group promotes animal-free science at society of toxicology conference
The PETA International Science Consortium Ltd. is presenting two posters on animal-free methods for testing inhalation toxicity at the 56th annual Society of Toxicology (SOT) meeting March 12 to 16, 2017, in Baltimore, Maryland.

Citizen Science in the Digital Age: Rhetoric, Science and Public Engagement
James Wynn's timely investigation highlights scientific studies grounded in publicly gathered data and probes the rhetoric these studies employ.

Science/Science Careers' survey ranks top biotech, pharma, and biopharma employers
The Science and Science Careers' 2016 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.

Three natural science professors win TJ Park Science Fellowship
Professor Jung-Min Kee (Department of Chemistry, UNIST), Professor Kyudong Choi (Department of Mathematical Sciences, UNIST), and Professor Kwanpyo Kim (Department of Physics, UNIST) are the recipients of the Cheong-Am (TJ Park) Science Fellowship of the year 2016.

Read More: Science News and Science Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to