Nav: Home

From urine samples to precision medicine in bladder cancer through 3D cell culture

July 30, 2019

A research collaboration led by scientists from institutions in Japan including Tokyo University of Agriculture and Technology (TUAT) has developed a new experimental cancer model for dog bladder cancer. Urine samples were used for a 3D cell culture method called organoid culture. This method will allow us to quickly determine the proper chemotherapy and to identify new biomarkers of both dog and human bladder cancer in the near future.

The researchers published their results on July 23rd in Cancer Science.

About 0.01% of humans suffer from bladder cancer. The most common symptom of cancer is blood in the urine and pain during urination. In the United States, 80,470 people are diagnosed with bladder cancer in 2019 and 17,670 are dead. About 90% of all bladder cancers are transitional cell carcinoma, which is usually invasive. In case of dogs, this cancer is detected very late, resulting in poor survival.

"For dogs, bladder cancer should be diagnosed as early as possible," said Tatsuya Usui, DVM, Ph.D., corresponding author on the paper and senior assistant professor in the Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine at TUAT in Japan. "It was, however, very hard to grow bladder cancer cells on flat dishes, which is called a traditional 2D cell culture method." Those researchers then tried a 3D cell culture method called organoid culture. They collected urine samples from bladder cancer dogs and successfully grew bladder cancer cells in urine using this culture system. These cells grown in the 3D cell culture express the same set of genes as the original cancer tissues do.

"Those results encouraged us to test anti-cancer drugs on cells grown in the 3D cell culture. As we expected, the sensitivity of each drug can be easily monitored, " said Usui. "It is now possible that bladder cancer cells from each dog using this system can be treated by several anti-cancer drugs. We can then find which drug is more effective for each dog in the lab before actual treatment. So we would like to apply the system of urine sample-derived dog bladder cancer 3D culture to precision veterinary medicine. In addition, we opened a new avenue for establishing the novel therapeutic strategy against urological cancer in both dog and human."
-end-
For more information about the Usui laboratory, please visit http://vet-pharmacol.com/

About Tokyo University of Agriculture and Technology (TUAT)

TUAT is a distinguished university in Japan dedicated to science and technology. TUAT focuses on agriculture and engineering that form the foundation of industry, and promotes education and research fields that incorporate them. Boasting a history of over 140 years since our founding in 1874, TUAT continues to boldly take on new challenges and steadily promote fields. With high ethics, TUAT fulfills social responsibility in the capacity of transmitting science and technology information towards the construction of a sustainable society where both human beings and nature can thrive in a symbiotic relationship. For more information, please visit http://www.tuat.ac.jp/en/.

Original publication:

Cancer Sci. 2019 Jun 29.
Establishment of a novel experimental model for muscle-invasive bladder cancer using a dog bladder cancer organoid culture.
Elbadawy M, Usui T, Mori T, Tsunedomi R, Hazama S, Nabeta R, Uchide T, Fukushima R, Yoshida T, Shibutani M, Tanaka T, Masuda S, Okada R, Ichikawa R, Omatsu T, Mizutani T, Katayama Y, Noguchi S, Iwai S, Nakagawa T, Shinohara Y, Kaneda M, Yamawaki H, Sasaki K.

doi: 10.1111/cas.14118.

Contact:

Tatsuya Usui, DVM, PhD.
Senior Assistant Professor
Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Agriculture, TUAT, Japan
E-mail: fu7085@go.tuat.ac.jp

Tokyo University of Agriculture and Technology

Related Science Articles:

PETA science group promotes animal-free science at society of toxicology conference
The PETA International Science Consortium Ltd. is presenting two posters on animal-free methods for testing inhalation toxicity at the 56th annual Society of Toxicology (SOT) meeting March 12 to 16, 2017, in Baltimore, Maryland.
AAAS and March for Science partner to uphold science
AAAS, the world's largest general scientific organization, announced Thursday that it will partner with the March for Science, a nonpartisan set of activities that aim to promote science education and the use of scientific evidence to inform policy.
Citizen Science in the Digital Age: Rhetoric, Science and Public Engagement
James Wynn's timely investigation highlights scientific studies grounded in publicly gathered data and probes the rhetoric these studies employ.
Science/Science Careers' survey ranks top biotech, pharma, and biopharma employers
The Science and Science Careers' 2016 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.
Three natural science professors win TJ Park Science Fellowship
Professor Jung-Min Kee (Department of Chemistry, UNIST), Professor Kyudong Choi (Department of Mathematical Sciences, UNIST), and Professor Kwanpyo Kim (Department of Physics, UNIST) are the recipients of the Cheong-Am (TJ Park) Science Fellowship of the year 2016.
More Science News and Science Current Events

Best Science Podcasts 2019

We have hand picked the best science podcasts for 2019. Sit back and enjoy new science podcasts updated daily from your favorite science news services and scientists.
Now Playing: TED Radio Hour

Erasing The Stigma
Many of us either cope with mental illness or know someone who does. But we still have a hard time talking about it. This hour, TED speakers explore ways to push past — and even erase — the stigma. Guests include musician and comedian Jordan Raskopoulos, neuroscientist and psychiatrist Thomas Insel, psychiatrist Dixon Chibanda, anxiety and depression researcher Olivia Remes, and entrepreneur Sangu Delle.
Now Playing: Science for the People

#537 Science Journalism, Hold the Hype
Everyone's seen a piece of science getting over-exaggerated in the media. Most people would be quick to blame journalists and big media for getting in wrong. In many cases, you'd be right. But there's other sources of hype in science journalism. and one of them can be found in the humble, and little-known press release. We're talking with Chris Chambers about doing science about science journalism, and where the hype creeps in. Related links: The association between exaggeration in health related science news and academic press releases: retrospective observational study Claims of causality in health news: a randomised trial This...