Modifying BACTRAC protocol provides first chance to study local leukocyte populations during stroke

August 07, 2020

FAIRFAX, Va. -- Expanding standard techniques during mechanical thrombectomy -- a procedure that removes a clot from an artery during stroke -- allows researchers to reproducibly obtain and study local leukocyte populations during human stroke, according to a study by the University of Kentucky Department of Neurology, released today at the Society of NeuroInterventional Surgery's (SNIS) 17th Annual Meeting.

The study, Changes in Leukocyte Distribution in Intracranial vs. Systemic Blood Collected during Mechanical Thrombectomy, started with the established BACTRAC protocol and modified the tissue collection protocol to isolate lymphocytes for flow cytometry and to bank the thrombus and plasma. The researchers first established the protocol in healthy controls using venous blood samples and then initiated for thrombectomy cases.

These methods can be used to expand our understanding of acute inflammatory mechanisms activated within the infarcted brain. This novel approach may be critical to identifying immunotherapeutic targets that can be delivered either as adjunctive therapies to mechanical thrombectomy, or in the phases of recovery after stroke.

"This modification to the existing BACTRAC protocol provides the opportunity, for the first time, to study changes in local leukocyte populations with flow cytometry in the arteries undergoing ischemic stroke in the human condition," said co-senior authors of the study Dr. Justin Fraser, Department of Neurosurgery, and Dr. Ann Stowe, Department of Neurology, both at the University of Kentucky. "Efficient processing of lymphocytes with subsequent flow cytometry analyses will provide important insight into the neuroinflammatory microenvironment of the occlusion during stroke."

Researchers will focus future studies on investigating changes in specific leukocyte populations and how they might relate to patient demographics, patient co-morbidities, infarct volume, and functional recovery. These data will help accelerate translational stroke research to illuminate new approaches for drug discovery and prognosis.
-end-
To receive a copy of this abstract or to speak with the authors, please contact Maria Enie at menie@vancomm.com or 202-248-5454.

About the Society of NeuroInterventional Surgery

The Society of NeuroInterventional Surgery (SNIS) is a scientific and educational association dedicated to advancing the specialty of neurointerventional surgery through research, standard-setting, and education and advocacy to provide the highest quality of patient care in diagnosing and treating diseases of the brain, spine, head, and neck. Visit http://www.snisonline.org and follow us on Twitter (@SNISinfo) and Facebook (@SNISOnline).

Society of NeuroInterventional Surgery

Related Stroke Articles from Brightsurf:

Stroke alarm clock may streamline and accelerate time-sensitive acute stroke care
An interactive, digital alarm clock may speed emergency stroke care, starting at hospital arrival and through each step of the time-sensitive treatment process.

Stroke patients with COVID-19 have increased inflammation, stroke severity and death
Stroke patients who also have COVID-19 showed increased systemic inflammation, a more serious stroke severity and a much higher rate of death, compared to stroke patients who did not have COVID-19, according a retrospective, observational, cross-sectional study of 60 ischemic stroke patients admitted to UAB Hospital between late March and early May 2020.

'Time is vision' after a stroke
University of Rochester researchers studied stroke patients who experienced vision loss and found that the patients retained some visual abilities immediately after the stroke but these abilities diminished gradually and eventually disappeared permanently after approximately six months.

More stroke awareness, better eating habits may help reduce stroke risk for young adult African-Americans
Young African-Americans are experiencing higher rates of stroke because of health conditions such as high blood pressure, diabetes and obesity, yet their perception of their stroke risk is low.

How to help patients recover after a stroke
The existing approach to brain stimulation for rehabilitation after a stroke does not take into account the diversity of lesions and the individual characteristics of patients' brains.

Kids with headache after stroke might be at risk for another stroke
A new study has found a high incidence of headaches in pediatric stroke survivors and identified a possible association between post-stroke headache and stroke recurrence.

High stroke impact in low- and middle-income countries examined at 11th World Stroke Congress
Less wealthy countries struggle to meet greater need with far fewer resources.

Marijuana use might lead to higher risk of stroke, World Stroke Congress to be told
A five-year study of hospital statistics from the United States shows that the incidence of stroke has risen steadily among marijuana users even though the overall rate of stroke remained constant over the same period.

We need to talk about sexuality after stroke
Stroke survivors and their partners are not adequately supported to deal with changes to their relationships, self-identity, gender roles and intimacy following stroke, according to new research from the University of Sydney.

Standardized stroke protocol can ensure ELVO stroke patients are treated within 60 minutes
A new study shows that developing a standardized stroke protocol of having neurointerventional teams meet suspected emergent large vessel occlusion (ELVO) stroke patients upon their arrival at the hospital achieves a median door-to-recanalization time of less than 60 minutes.

Read More: Stroke News and Stroke Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.