A phylogenetic analysis reveals the evolution of the mitochondrial calcium transporter

August 12, 2020

The system that regulates cellular calcium levels duplicated, generating two non-equivalent systems, some one billion years ago before fungi and animals diverged evolutionarily.

The fungal models currently used for the study of mitochondrial calcium regulation are not adequate, as the system they possess is not equivalent to that of animals. Chytrids, a divergent group of fungi, would be the only fungi that possess a system similar to ours.

The study by IRB Barcelona's Comparative Genomics Laboratory has been published in the journal Nature Communications.

Calcium levels regulate multiple processes in cells, ranging from metabolism to division. And these levels are regulated, in turn, by calcium transport into and out of mitochondria, the energy hub of the cell. The regulation of calcium levels is, therefore, a recurring topic of study, with important implications in biomedicine. In this context, fungi are often the model organisms of choice to study this system.

The Comparative Genomics Laboratory of the Institute for Research in Biomedicine (IRB Barcelona), headed by ICREA researcher Toni Gabaldón, and the Barcelona Supercomputing Center (BSC-CNS) has revealed that the transporter that regulates cellular calcium levels duplicated around one billion years ago, giving rise to two transporter subtypes. Of these, only one is present in animals, while most fungi conserve the other. Chytrids--aquatic microscopic organisms--are the only family in the fungal kingdom that has the same transporter subtype as animals. Therefore, the use of the calcium transporter of any other kind of fungus as a model could lead to incoherent observations and incorrect conclusions.

"Determining how cell systems came about and evolved helps us to understand them and to choose appropriate model organisms for research purposes," says Gabaldón.

An extensive computational analysis of more than 1000 organisms

Published in the journal Nature Communications, this study used computational methods to analyse the complete genome of more than 1000 organisms, in order to reconstruct the evolution of the calcium transport machinery in mitochondria. The analysis corroborated the ancestral origin of this mitochondrial transport system, which was already present in the common ancestor of fungi and animals.

Fabiana Perochi's laboratory at the Helmholtz Zentrum in Munich (Germany) carried out experimental tests that confirmed that chytrid proteins perform a function equivalent to that of humans.

The paradox of fungi that do not transport calcium

It had previously been observed that the calcium transporter in fungi acts differently from that in animals and it's even present in organisms that do not transport calcium to mitochondria. This observation was considered a paradox.

"By reconstructing the evolutionary history of these proteins, we have been able to resolve a paradox and identify the true equivalents of the human system in fungi. This finding opens up new avenues for their use as experimental models," concludes Gabaldón.
-end-


Institute for Research in Biomedicine (IRB Barcelona)

Related Evolution Articles from Brightsurf:

Seeing evolution happening before your eyes
Researchers from the European Molecular Biology Laboratory in Heidelberg established an automated pipeline to create mutations in genomic enhancers that let them watch evolution unfold before their eyes.

A timeline on the evolution of reptiles
A statistical analysis of that vast database is helping scientists better understand the evolution of these cold-blooded vertebrates by contradicting a widely held theory that major transitions in evolution always happened in big, quick (geologically speaking) bursts, triggered by major environmental shifts.

Looking at evolution's genealogy from home
Evolution leaves its traces in particular in genomes. A team headed by Dr.

How boundaries become bridges in evolution
The mechanisms that make organisms locally fit and those responsible for change are distinct and occur sequentially in evolution.

Genome evolution goes digital
Dr. Alan Herbert from InsideOutBio describes ground-breaking research in a paper published online by Royal Society Open Science.

Paleontology: Experiments in evolution
A new find from Patagonia sheds light on the evolution of large predatory dinosaurs.

A window into evolution
The C4 cycle supercharges photosynthesis and evolved independently more than 62 times.

Is evolution predictable?
An international team of scientists working with Heliconius butterflies at the Smithsonian Tropical Research Institute (STRI) in Panama was faced with a mystery: how do pairs of unrelated butterflies from Peru to Costa Rica evolve nearly the same wing-color patterns over and over again?

Predicting evolution
A new method of 're-barcoding' DNA allows scientists to track rapid evolution in yeast.

Insect evolution: Insect evolution
Scientists at Ludwig-Maximilians-Universitaet (LMU) in Munich have shown that the incidence of midge and fly larvae in amber is far higher than previously thought.

Read More: Evolution News and Evolution Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.