Molecular 'Radar' Tracks Key Process In Embryonic Development

August 14, 1997

REHOVOT, Israel - August 22, 1997 - A molecular "radar" that makes it possible to track signaling enzymes inside a cell in real time has been developed at the Weizmann Institute of Science. Using the "radar," the scientists mapped the exact progress of an intercellular messenger that plays a key role in embryonic development.

The achievement, reported in the August 22 issue of Science and featured on the journal's cover, is expected to prove valuable in gaining a better understanding of how signals are transferred inside a cell and how the signaling process goes awry in diseases, such as cancer.

"Previously, in studying message transmission inside the cells of a developing organism, we scientists were rather like people at an airport watching the planes take off and land," says research team leader Prof. Ben-Zion Shilo who heads the Institute's Molecular Genetics Department. "We could make some intelligent inferences about where the planes were going or where they had come from, but we couldn't see the course a plane was following.

"Our new method gives us the ability equivalent to that of an air traffic controller who looks at the dots on the radar screen and can thus follow the movements of each plane step by step.

"We can suddenly look at processes in a cell or an embryo as they are happening and don't have to infer things from the consequences any more," Shilo says.

Prof. Shilo conducted the study with Dr. Rony Seger of the Membrane Research and Biophysics Department and with doctoral student Limor Gabay of the Molecular Genetics Department.

Observing messengers in real time

The starting point for the study was the knowledge that many messages inside cells are passed on by means of phosphate atoms.

When a molecular messenger, such as a hormone, attaches itself to a receptor on the cell membrane, it sets off a chain reaction inside the cell. In its course, one molecule activates the next, and so on, through the addition of phosphate atoms, a process known as phosphorylation.

To track the activated, phosphate-containing molecules, the team developed antibodies that react only with molecules phosphorylated in a particular fashion. Since these antibodies can be easily traced, the system allowed the scientists to actually observe phosphorylation - hence, the pathway of signal transmission - in real time.

Shilo and his team worked with Drosophila fruit flies. These insects are commonly used in scientific research because they share many genetic and molecular characteristics with higher animals, develop rapidly and are easy to study. The researchers focused on a hormone-like messenger called epidermal growth factor (EGF), which becomes active during embryonic development and ensures the formation of a proper body pattern.

Using the new method, they followed the signal transmitted by EGF from the point at which EGF attaches to its receptor on the cell membrane up to the time it delivers the message to the genes in the cell nucleus. They were able to see precisely when and where the signal is passed on within individual cells, and also to observe which cells within the embryo are affected by EGF at different stages of embryonic development.

"We can trace signals in several cells simultaneously and chart an atlas of signal transmission for the entire embryo," says Shilo.

Preventing abnormal development

The new molecular "radar" also is a valuable tool for studying phosphorylation patterns set off by other receptors, and for investigating phosphorylation in other organisms, including humans. It can shed light on both normal development and abnormal tissue growth, such as in cancer.

"Phosphorylation is universal in living organisms and is part of normal growth and development. But in many types of cancer there is deregulated phosphorylation, causing uncontrolled cell growth," says Shilo.

"Clearly, we can use this method to track the phosphorylation pattern in these diseases, and it could be a useful diagnostic tool to find where things are going wrong," says Shilo. "And if you can see where things are going wrong you can set about finding specific ways to stop them."

Dr. Seger holds the Samuel and Isabelle Friedman Career Development Chair. Antibodies for this research were developed in collaboration with Sigma Israel Chemicals Ltd. The study was supported in part by the Dr. Josef Cohn Minerva Center for Biomembrane Research at the Weizmann Institute and by grants from the Tobacco Research Council of the United States, the US-Israel Binational Science Foundation, the UK-Israel Science and Technology Research Fund and the Minerva Foundation, Munich, Germany.

The Weizmann Institute of Science, in Rehovot, Israel, is one of the world's foremost centers of scientific research and graduate study. Its 2,400 scientists, students, technicians, and engineers pursue basic research in the quest for knowledge and the enhancement of the human condition. New ways of fighting disease and hunger, protecting the environment, and harnessing alternative sources of energy are high priorities.
-end-


American Committee for the Weizmann Institute of Science

Related Cell Membrane Articles from Brightsurf:

Lighting the way to selective membrane imaging
A team of scientists at Kanazawa University have shown how water-soluble tetraphenylethene molecules can become fluorescent when aggregating at a biomembrane-mimetic liquid-liquid interface.

What membrane can do in dealing with radiation
USTC recently found that polymethylmethacrylate (PMMA) and polyvinyl chloride (PVC) can release acidic substance under γ radiation, whose amount is proportional to the radiation intensity.

Bioelectronic device achieves unprecedented control of cell membrane voltage
Every living cell maintains a voltage across the cell membrane that results from differences in the concentrations of charged ions inside and outside the cell.

Novel cell membrane model could be key to uncovering new protein properties
Researchers have recently shed light on how cell membrane proteins could be influenced by the lipids around them.

Using light's properties to indirectly see inside a cell membrane
Using properties of light from fluorescent probes is at the heart of a new imaging technique developed at Washington University's McKelvey School of Engineering that allows for an unprecedented look inside cell membranes.

Cell 'membrane on a chip' could speed up screening of drug candidates for COVID-19
Researchers have developed a human cell 'membrane on a chip' that allows continuous monitoring of how drugs and infectious agents interact with our cells, and may soon be used to test potential drug candidates for COVID-19.

Scientists synthesize novel artificial molecules that mimic a cell membrane protein
Scientists at Tokyo Institute of Technology (Tokyo Tech) recently developed an artificial transmembrane ligand-gated channel that can mimic the biological structure and function of its natural counterpart.

Across the cell membrane
Aquaporins and glucose transporters facilitate the movement of substances across biological membranes and are present in all kingdoms of life.

Location, location, location: The cell membrane facilitates RAS protein interactions
Many cancer medications fail to effectively target the most commonly mutated cancer genes in humans, called RAS.

New self-forming membrane to protect our environment
A new class of self-forming membrane has been developed by researchers from Newcastle University, UK.

Read More: Cell Membrane News and Cell Membrane Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.