Scientists combine chemistry and bioremediation to clean cadmium from soil

August 23, 2000

Scientists at the U.S. Department of Energy's Brookhaven National Laboratory have devised a way to combine chemical treatment with "pollutant-busting" bacteria to remove cadmium from contaminated soil. They will present their latest data at the 220th national meeting of the American Chemical Society in Washington, D.C., on Thursday, August 24, 2000, at 10:10 a.m., in the Grand Hyatt Independence Ballroom D-E.

"Environmental contamination by heavy metals such as cadmium is a serious and growing concern," says environmental chemist Murthy A. Vairavamurthy, who leads the research at Brookhaven. Cadmium is a highly toxic and carcinogenic metal used in metal plating, nickel-cadmium batteries, pigments, plastic stabilizers, pesticides and more. It is most toxic in its free (ionic) form, which easily dissolves and moves through water, threatening groundwater supplies.

There are two basic ways to deal with soil contaminated with such toxic heavy metals, Vairavamurthy says. One is to mobilize the metals so they can be extracted from the soil and treated. The other is to convert the metals to a stable form that will not migrate into groundwater. To find ways to accomplish one or the other, the Brookhaven team looked for clues from nature.

"Many organisms use thiols - organic sulfhydryl compounds - to detoxify metals in the body," Vairavamurthy says. Many thiols form soluble complexes with toxic metals, which can then be excreted. "We thought these might work in soil as well," he says.

The team was also intrigued by the natural ability of many microbes to detoxify contaminants. "Bacteria have versatile biochemical mechanisms, which we thought we might be able to exploit intelligently," Vairavamurthy explains.

In the end, the team has proposed a method that combines both approaches:

First, thiosulfate, an inorganic compound with a sulfhydryl group, is injected into the subsurface soil. Thiosulfate has an affinity for and forms highly soluble complexes with cadmium. Then, when water is pumped out of the soil, the dissolved cadmium thiosulfate should come along for the ride.

The extracted water then can be treated with a new form of bacteria, the Cd-1 strain of Klebsiella planticola, which was recently isolated by former BNL researcher Pramod Sharma from coastal salt marshes on Shelter Island in New York. Through its normal metabolic process, the bacterium converts dissolved cadmium thiosulfate to cadmium sulfide, an insoluble form, which precipitates out of the solution.

Alternatively, the bacteria could also be injected directly into the soil following thiosulfate injection to complete the process in situ. This would leave insoluble cadmium sulfide in place of the original toxic metal. "Cadmium sulfide is fairly stable and will not move into the groundwater as long as the conditions remain anaerobic," or without oxygen, Vairavamurthy says.

This in situ technique would potentially be less costly than digging up contaminated soil for treatment elsewhere, and even less costly than the water-pumping method described above. Also, because these bacteria can grow in oxygen-rich as well as oxygen-poor environments, they can be grown in large quantity under normal aerobic conditions in the laboratory, and still survive the oxygen-poor environment below the soil surface.

The same technique might also work for treating soil contaminated with some other metals, such as arsenic and cobalt, Vairavamurthy says.
This research was funded by the Natural and Accelerated Bioremediation Program of the U.S. Department of Energy.

The U.S. Department of Energy's Brookhaven National Laboratory creates and operates major facilities available to university, industrial and government personnel for basic and applied research in the physical, biomedical and environmental sciences and in selected energy technologies. The Laboratory is operated by Brookhaven Science Associates, a not-for-profit research management company, under contract with the U.S. Department of Energy.

Note to local editors: Murthy A. Vairavamurthy lives in Shoreham, New York.

DOE/Brookhaven National Laboratory

Related Bacteria Articles from Brightsurf:

Siblings can also differ from one another in bacteria
A research team from the University of Tübingen and the German Center for Infection Research (DZIF) is investigating how pathogens influence the immune response of their host with genetic variation.

How bacteria fertilize soya
Soya and clover have their very own fertiliser factories in their roots, where bacteria manufacture ammonium, which is crucial for plant growth.

Bacteria might help other bacteria to tolerate antibiotics better
A new paper by the Dynamical Systems Biology lab at UPF shows that the response by bacteria to antibiotics may depend on other species of bacteria they live with, in such a way that some bacteria may make others more tolerant to antibiotics.

Two-faced bacteria
The gut microbiome, which is a collection of numerous beneficial bacteria species, is key to our overall well-being and good health.

Microcensus in bacteria
Bacillus subtilis can determine proportions of different groups within a mixed population.

Right beneath the skin we all have the same bacteria
In the dermis skin layer, the same bacteria are found across age and gender.

Bacteria must be 'stressed out' to divide
Bacterial cell division is controlled by both enzymatic activity and mechanical forces, which work together to control its timing and location, a new study from EPFL finds.

How bees live with bacteria
More than 90 percent of all bee species are not organized in colonies, but fight their way through life alone.

The bacteria building your baby
Australian researchers have laid to rest a longstanding controversy: is the womb sterile?

Hopping bacteria
Scientists have long known that key models of bacterial movement in real-world conditions are flawed.

Read More: Bacteria News and Bacteria Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to