Size matters: How cells pack in epithelial tissues

September 05, 2019

KANSAS CITY, MO--Small-cell clones in proliferating epithelia - tissues that line all body surfaces - organize very differently than their normal-sized counterparts, according to a recent study from the Stowers Institute for Medical Research. Published online September 5, 2019, in Developmental Cell, these findings from the laboratory of Matthew Gibson, PhD, may contribute to a better understanding of how some human diseases progress.

"A common feature of many cancer types is the pleomorphic nature - or variability in size and shape - of cells in a tumor," says first author and Postdoctoral Research Associate Subramanian P. Ramanathan, PhD. "What's not exactly known is whether differential cell size is the driver for, or the result of, cancer progression."

Cells in an epithelial sheet are normally connected by Velcro-like structures called adhesive junctions and have a near-uniform size distribution. As a result, cells related by descent tend to stick together in mosaic patches referred to as cell clones. In the fruit fly Drosophila melanogaster, epithelial cell clones that have smaller cells can lose contact with each other and disperse among their neighbors.

The striking dispersal of small cells carrying mutations in a gene called Tor was first observed over a decade ago by Gibson when he was a postdoctoral researcher. "I puzzled over it, but just couldn't make sense of why the mutant cells dispersed within the cell layer," says Gibson. Not until 2015, when Ramanathan joined the lab, did they begin to piece together the science behind this observation.

Ramanathan has a background in the biomechanics of cell division. He recalls, "we were very excited about the prospect of applying my interest in single-cell mechanics in the context of how epithelial cells organize."

"We initially thought we were going to be studying cell division and its relationship to junction formation," says Gibson. "But based on Subramanian's experiments, we determined that the Tor cells divide and make a junction that later becomes unstable. He was able to rule out cytoskeletal or biophysical explanations, and that led to a mathematical treatment of the problem."

"The eureka moment was the soap bubbles experiment," says Ramanathan, citing D'Arcy Wentworth Thompson's, On Growth and Form. "About a hundred years ago, Thompson documented the similarity between how soap foam and biological tissue organize. Soap cells grow and shrink in an extremely predictable fashion. We found that the junctions shared by smaller soap cells frequently collapsed separating the small cells. This was strikingly similar to what we saw in epithelial tissue," says Ramanathan.

Ramanathan reasoned that the Tor cell dispersal could be a geometrical consequence. "We had to convince ourselves that this is what was happening," he admits. To explore it in the purely in silico framework, they reached out to co-author Matej Krajnc, PhD, from Princeton University, who has experience with these types of models. "He was excited to modulate cell size, to see if we could see similar patterns emerging in silico. And that is indeed what we saw."

One of the most astounding feats of developmental biology is the transformation of an epithelial sheet into a functionally specialized three-dimensional structure. To do so, epithelial systems rely on uniformity among cells. Developmental processes shape and sculpt epithelia by creating region-specific properties that are classically understood to be driven by molecular pathways.

"In the human body, which is primarily made up of epithelial cell layers, the vast majority of mutations we accumulate are not germline, but rather somatic, and therefore clonal in nature," says Gibson. "In a pathogenesis context, because mutations create clonal effects, we don't have a framework for thinking about how clones of cells behave when they're genetically distinct from the surrounding wild type cells, but it's extremely important." The Gibson Lab continues to build off this study and related work to understand the interaction between physically and genetically heterogeneous epithelial cell populations.
This research was funded by the Stowers Institute and a grant from the National Institute of General Medical Sciences of the National Institutes of Health under award number R01GM111733-05. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Lay Summary of Findings

Research from the lab of Matthew Gibson, PhD, of the Stowers Institute for Medical Research, highlights the importance of uniform cell size in maintaining the architecture of epithelial sheets. Published online September 5, 2019, in Developmental Cell, these findings may further the understanding of human disease progression.

Using molecular genetic methods and live imaging, as well as in silico and soap bubble modeling, first author Subramanian P. Ramanathan, PhD, and colleagues show that abnormally small epithelial cells can dissociate from each other and disperse amongst normal cells. A series of experiments show that abnormal small-cell dispersal results from geometric effects of cell size variation on epithelial packing. Ongoing research by Ramanathan aims to determine whether abnormally small cells may have a role in disease initiation or progression, such as in tumorigenesis.

About the Stowers Institute for Medical Research

The Stowers Institute for Medical Research is a non-profit, basic biomedical research organization dedicated to improving human health by studying the fundamental processes of life. Jim Stowers, founder of American Century Investments, and his wife, Virginia, opened the Institute in 2000. Currently, the Institute is home to about 500 researchers and support personnel, over 20 independent research programs, and more than a dozen technology development and core facilities. Learn more about the Institute at and its graduate program at

Stowers Institute for Medical Research

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