Nav: Home

'Clamp' regulates message transfer between mammal neurons

September 09, 2019

MADISON - A fundamental question in nerve biology brings to mind a race car at the starting line: The engine is revving, but the brake is on. The system is ready to go, but under tight control.

And when the light flashes green, the car blasts away from the line.

A similar process occurs at junctions between two nerve cells when a different type of brake is disengaged. At that point, the machinery controlling the release of nerve signaling chemicals open a "fusion pore" that allows the escape of these neurotransmitters. Only then is a signal -- contained within tiny packages called vesicles -- released to other neurons.

Today, in the journal Nature Communications, Edwin Chapman, a professor of neuroscience at University of Wisconsin-Madison, has described a key component of the system -- the brake, or "clamp," that prevents the fusion pore from completing its formation and opening.

"We need the vesicles to release their contents, yes," says Chapman, a Howard Hughes Medical Institute investigator. "But release is not always appropriate. Preventing the pore from fully forming and opening is just as critical. But today, the physiology of clamping -- or inhibiting -- the pore is hotly debated. We believe we have a conclusive answer to what happens in mammal neurons, and it's different from what others have found based on studies of invertebrate neurons."

The fusion pore forms in synapses, the junctions between adjacent neurons. The pore, comprising "SNARE" proteins, cannot release a signal until a spritz of calcium ions triggers the opening. The calcium sensor is a protein called synaptotagmin which, Chapman has now found, is also key to the fusion clamp.

Transmissions across synapses underlie every function of the nervous system: signaling muscle movement, recalling a memory of childhood, feeling a hot stove or thinking through a calculus problem. Each of these builds on a complex choreography of nerve signals passed from one neuron to another by neurotransmitters contained in vesicles that are released through fusion pores.

The discovery that pore formation requires SNARE proteins won the 2013 Nobel Prize. But Chapman and others have raised a critical question: If SNAREs are so attuned to making pores, why are the pores usually closed?

"There has been a tremendous amount of attention on the synapse, but no conclusive answer," he says. "What keeps the SNAREs from forming open fusion pores? What is the brake, the clamp? What shuts it off until the appropriate time?"

In Nature Communications, Chapman, recent postdoctoral researcher Nicholas Courtney and colleagues reported on their studies of two candidate clamp molecules: complexin and a type of synaptotagmin called syt-1.

"There is this strong evidence, from fruit flies and worms, that complexin is the clamp," Chapman says. "But our new study rules out that role in mammals.

"There has been a lot of confusion, because the different model systems give different results," Chapman adds. "But we took a long, comprehensive look, in one model system -- mammalian cells in a dish. The question was: Is complexin or syt-1 the clamp? Or do they work together?"

In mammals, he found, complexin cannot be the fusion clamp because it plays a positive role, actually helping to open the pore. Curiously, complexin is aided in this function by syt-1.

But syt-1 by itself is able to clamp the pore.

"Syt-1 thus is the long-sought brake that prevents errant signals across a synapse," Chapman says.

The race-car analogy is apt, Chapman says, since hair-trigger speed is necessary in the nervous system.

"Nerve terminals have the tightest regulation -- at millisecond accuracy -- in our physiology," he says. "When the kidney is making urine, it does not need such accuracy. But neurons, and the neuroendocrine cells [like those that produce adrenaline] are specialized for speed. You need to have them primed, waiting for the trigger. The synapse is held in an arrested state, and boom! It gets the signal, and the signal passes. The speed of synapses determines how fast our brains work. This is important stuff."

In 2018, Chapman's lab showed that fusion pores are not simply pipes, but instead highly tuned valves with a full range of openings from off to intermittent, to full open, depending on the proteins present. In 2019, they showed how a different synaptotagmin, syt-17, helped trigger the growth of axons -- the slender nerve fibers that convey signals to synapses, and then to dendrites on a neighboring neuron.

Neurons need fusion clamps as much as cars need brakes, Chapman emphasizes. "If the rate of fusion - the transfer of signals - is dis-regulated, the nervous system will not work properly," he says.

Fusion pores aren't just gates between neurons. They connect membranes between biological units inside and outside cells.

"The need to make membranes fuse and separate underlies all of cell biology for organisms with a cell nucleus," Chapman says. "When you slice a cell, it's made of myriad compartments, each separated by a membrane. For cells to work, there needs to be a constant process of fission and fusion involving fusion pores."

In neurological disease, "the release of neurotransmitters can be an important factor," Chapman adds. "If there is too little, we might be able to design a drug to affect the machinery. Or do the opposite if there is too much release. That's the ultimate point of this basic research - to find ways to make life better for people in need."
This research was supported by National Institutes of Health grants MH061876 and NS097362.

-- David Tenenbaum, 608-265-8549,

University of Wisconsin-Madison

Related Neurons Articles:

Shaping the social networks of neurons
Identification of a protein complex that attracts or repels nerve cells during development.
With these neurons, extinguishing fear is its own reward
The same neurons responsible for encoding reward also form new memories to suppress fearful ones, according to new research by scientists at The Picower Institute for Learning and Memory at MIT.
How do we get so many different types of neurons in our brain?
SMU (Southern Methodist University) researchers have discovered another layer of complexity in gene expression, which could help explain how we're able to have so many billions of neurons in our brain.
These neurons affect how much you do, or don't, want to eat
University of Arizona researchers have identified a network of neurons that coordinate with other brain regions to influence eating behaviors.
Mood neurons mature during adolescence
Researchers have discovered a mysterious group of neurons in the amygdala -- a key center for emotional processing in the brain -- that stay in an immature, prenatal developmental state throughout childhood.
Astrocytes protect neurons from toxic buildup
Neurons off-load toxic by-products to astrocytes, which process and recycle them.
Connecting neurons in the brain
Leuven researchers uncover new mechanisms of brain development that determine when, where and how strongly distinct brain cells interconnect.
The salt-craving neurons
Pass the potato chips, please! New research discovers neural circuits that regulate craving and satiation for salty tastes.
When neurons are out of shape, antidepressants may not work
Selective serotonin reuptake inhibitors (SSRIs) are the most commonly prescribed medication for major depressive disorder (MDD), yet scientists still do not understand why the treatment does not work in nearly thirty percent of patients with MDD.
Losing neurons can sometimes not be that bad
Current thinking about Alzheimer's disease is that neuronal cell death in the brain is to blame for the cognitive havoc caused by the disease.
More Neurons News and Neurons Current Events

Trending Science News

Current Coronavirus (COVID-19) News

Top Science Podcasts

We have hand picked the top science podcasts of 2020.
Now Playing: TED Radio Hour

There's so much we've yet to explore–from outer space to the deep ocean to our own brains. This hour, Manoush goes on a journey through those uncharted places, led by TED Science Curator David Biello.
Now Playing: Science for the People

#555 Coronavirus
It's everywhere, and it felt disingenuous for us here at Science for the People to avoid it, so here is our episode on Coronavirus. It's ok to give this one a skip if this isn't what you want to listen to right now. Check out the links below for other great podcasts mentioned in the intro. Host Rachelle Saunders gets us up to date on what the Coronavirus is, how it spreads, and what we know and don't know with Dr Jason Kindrachuk, Assistant Professor in the Department of Medical Microbiology and infectious diseases at the University of Manitoba. And...
Now Playing: Radiolab

Dispatch 1: Numbers
In a recent Radiolab group huddle, with coronavirus unraveling around us, the team found themselves grappling with all the numbers connected to COVID-19. Our new found 6 foot bubbles of personal space. Three percent mortality rate (or 1, or 2, or 4). 7,000 cases (now, much much more). So in the wake of that meeting, we reflect on the onslaught of numbers - what they reveal, and what they hide.  Support Radiolab today at