Chemokine expression heightens antibacterial immunity

September 12, 2001

Macrophage-derived chemokine (MDC) acts preferentially on Th2 lymphocytes, promoting their migration and setting the stage for antigen-specific B cell responses. Although this chemokine is expressed at low constitutive levels in antigen-presenting cells, Kikuchi and Crystal hypothesized that increasing its expression in dendritic cells (DCs) could allow for more efficient T cell help and a more effective humoral response. Since antibodies are key to the suppression of bacterial infections, these authors tested their idea in a mouse model of bacterial pneumonia. They transduced DCs with a human MDC cDNA and exposed the transgenic cells to pathogenic Pseudomonas bacteria, allowing the cells to process and display bacterial antigens. When reintroduced into host mice, these antigen-pulsed DCs effectively suppressed otherwise lethal infections with Pseudomonas. As expected, passive transfer of CD4 cells that had been activated by these DCs conferred the same protection to naive hosts, and even simple transfer of the serum from DC recipient animals allowed otherwise untreated recipients to combat the infection. Control DCs that were exposed to the pathogen but that express only endogenous, low levels of MDC conferred only minimal protection to recipient animals. This approach allows for specific immunization against this one pathogenic species, but it is fully effective only against certain clinical isolates. Hence if this approach is to be tried for clinical purposes, it may be necessary to expose DCs to multiple bacterial strains. Kikuchi and Crystal also note that increased MDC expression by DCs could promote undesired Th2-dependent immune responses, such as those associated with allergic symptoms.

JCI Journals

Related Antibodies Articles from Brightsurf:

Scientist develops new way to test for COVID-19 antibodies
New research details how a cell-free test rapidly detects COVID-19 neutralizing antibodies and could aid in vaccine testing and drug discovery efforts.

Mussels connect antibodies to treat cancer
POSTECH research team develops innovative local anticancer immunotherapy technology using mussel protein.

For an effective COVID vaccine, look beyond antibodies to T-cells
Most vaccine developers are aiming solely for a robust antibody response against the SARS-CoV-2 virus, despite evidence that antibodies are not the body's primary protective response to infection by coronaviruses, says Marc Hellerstein of UC Berkeley.

Children can have COVID-19 antibodies and virus in their system simultaneously
With many questions remaining around how children spread COVID-19, Children's National Hospital researchers set out to improve the understanding of how long it takes pediatric patients with the virus to clear it from their systems, and at what point they start to make antibodies that work against the coronavirus.

The behavior of therapeutic antibodies in immunotherapy
Since the late 1990s, immunotherapy has been the frontline treatment against lymphomas where synthetic antibodies are used to stop the proliferation of cancerous white blood cells.

Re-engineering antibodies for COVID-19
Catholic University of America researcher uses 'in silico' analysis to fast-track passive immunity

Seroprevalence of antibodies to SARS-CoV-2 in 10 US sites
This study estimates how common SARS-CoV-2 antibodies are in convenience samples from 10 geographic sites in the United States.

Neutralizing antibodies in the battle against COVID-19
An important line of defense against SARS-CoV-2 is the formation of neutralizing antibodies.

Three new studies identify neutralizing antibodies against SARS-CoV-2
A trio of papers describes several newly discovered human antibodies that target the SARS-CoV-2 virus, isolated from survivors of SARS-CoV-2 and SARS-CoV infection.

More effective human antibodies possible with chicken cells
Antibodies for potential use as medicines can be made rapidly in chicken cells grown in laboratories.

Read More: Antibodies News and Antibodies Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to