USC researchers hone in on the elusive receptor for sour taste

September 19, 2019

Sour is the taste of summer, a taste that evokes lemonade stands and vine-ripe tomatoes. Among the five basic tastes -- the others being bitter, sweet, salty and umami -- it is arguably the most subtle. In small amounts, it adds a critical tang to an otherwise bland dish. At higher concentrations and on its own, it's unpleasant or even painful.

But what causes the sensation of sourness, and why do we like it so much? USC scientists may have solved the first mystery: how sour tastes are sensed by animals.

Fruits and vegetables that taste sour are high in acids, including citric acid for lemons, tartaric acid for grapes and acetic acids in fermented foods like vinegar. It has been recognized for more than a century -- since the introduction of the pH meter -- that the low pH and high concentration of H+ ions in these foods generate a perception of sourness in humans. But how pH is sensed at the level of the tongue, and specifically what molecule constitutes the pH sensor, was not known.

A group led by Emily Liman, professor of biological sciences at the USC Dornsife College of Letters, Arts & Sciences, have reported in Current Biology that a sensor for pH on the tongue is the otopetrin 1 gene (Otop1). Otop1 is a member of a class of molecules called ion channels, which allow charged ions to cross cell membranes. In the case of Otop1, the charged ion carried across the membrane is H+, which is released into the mouth by acids.

Last year, Liman's team published research in Science that closed in on the sour-taste sensor. In that study, they used high-throughput sequencing methods made possible by advances in genomics to identify a list of roughly 40 previously uncharacterized genes that could encode a sour sensor. By studying the function of each gene, they whittled the list down to Otop1 because it was the only candidate that, when introduced into non-taste cells, gave them the ability to respond to acids.

While the USC scientists had identified OTOP1 -- the protein encoded by the Otop1 gene -- as forming a proton channel, they did not show that it was required for sour-taste responses in an intact animal.

Taste occurs when ingested chemicals interact with specialized cells on the tongue and palate. These cells are called taste receptor cells and are found in taste buds, which are concentrated on the back, sides and front of the tongue and on the roof of the mouth. Different taste receptor cells respond to each of the five basic tastes, and they release neurotransmitters onto gustatory nerves that send signals to the brain. This allows the nervous system to determine whether the ingested chemical has qualities perceived as bitter, sweet, umami, sour, salty or a mix of the five.

The new study followed up on previous findings that OTOP1 gave cells the ability to detect low pH. Graduate student Yu-Hsiang Tu used gene-editing technology to generate mice with an inactivated Otop1 gene to test if the OTOP1 protein was necessary for responding to sour chemicals -- or acids. When sour taste receptor cells are exposed to acids, they respond by producing an electrical signal -- or current -- because of the movement of H+ ions across the cell's membrane.

Graduate student Bochuan Teng showed that the sour taste receptor cells from the mice with nonfunctional OTOP1 did not have detectable currents representing the movement of H+ into cells. The sour taste receptor cells from the mutant mice also did not decrease their intracellular pH when exposed to acids, which would happen if H+ ions moved into the cell. Finally, the sour taste receptor cells from the mutant mice did not produce action potentials -- another electrical signal -- which are needed to activate the gustatory nerve and signal to the brain in response to some acidic solutions.

Whereas the previous experiments were performed with isolated taste receptor cells, the researchers also studied the importance of OTOP1 in mice by measuring the activity of the gustatory nerves in response to sour-tasting solutions introduced into the mouth of the mice. For these experiments, they teamed up with leading taste researcher Sue Kinnamon and graduate student Courtney Wilson at the University of Colorado Medical School. As expected, the activity of these nerves was severely reduced in mice with nonfunctional OTOP1, showing that the ability of the mice to sense acidic solutions -- and thus sour tastes -- was impaired.

"Our results show that OTOP1 is a bona fide sour taste receptor," Liman said. "This is the first definitive evidence for a protein that is both necessary and sufficient for sour taste receptor cells to respond to acids and stimulate the nerves to enable sour taste perception."

Surprisingly, the scientists found that mice with a nonfunctional Otop1 gene could still produce a small response to sour taste stimuli; the sour taste receptor cells still produced a few action potentials and the gustatory nerve produced a small response to very acidic stimuli. They postulate that another signaling mechanism, unrelated to OTOP1, also contributes to sour taste. They also tested the behavior in mice and found that the mice with a nonfunctional Otop1 gene still found acidic stimuli aversive.

"The behavioral response to acidic stimuli that are ingested is complex. You have the taste receptor cells that can detect acids, but you also have the pain system, which responds to low pH," Liman said. "Finding the molecular basis for the sour taste sensor takes us one step closer to understanding how different animals and individuals perceive the world."

Identification of the molecule responsible for taste opens up possibilities for wide application. This information may lead to an understanding of individual differences in food preferences and flavor perception, guide nutrition science and lead to novel approaches to pest control. Professional flavorists and chemists can leverage this information to manipulate flavors to make food or even medications more pleasing to the palate while also making household products containing toxic chemicals less pleasing.
Liman is the lead author of the study, joined by co-authors Bochuan Teng and Yu-Hsiang Tu of USC Dornsife College; Courtney E. Wilson and Sue C. Kinnamon of University of Colorado Medical School; and Narendra R. Joshi of Harvard Medical School.

This work was supported by NIH grants (#R01 DC013741, #R01 DC012555, #F31 DC015700 and #DP1 AT009497) and the Howard Hughes Medical Institute.

University of Southern California

Related Science Articles from Brightsurf:

75 science societies urge the education department to base Title IX sexual harassment regulations on evidence and science
The American Educational Research Association (AERA) and the American Association for the Advancement of Science (AAAS) today led 75 scientific societies in submitting comments on the US Department of Education's proposed changes to Title IX regulations.

Science/Science Careers' survey ranks top biotech, biopharma, and pharma employers
The Science and Science Careers' 2018 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.

Science in the palm of your hand: How citizen science transforms passive learners
Citizen science projects can engage even children who previously were not interested in science.

Applied science may yield more translational research publications than basic science
While translational research can happen at any stage of the research process, a recent investigation of behavioral and social science research awards granted by the NIH between 2008 and 2014 revealed that applied science yielded a higher volume of translational research publications than basic science, according to a study published May 9, 2018 in the open-access journal PLOS ONE by Xueying Han from the Science and Technology Policy Institute, USA, and colleagues.

Prominent academics, including Salk's Thomas Albright, call for more science in forensic science
Six scientists who recently served on the National Commission on Forensic Science are calling on the scientific community at large to advocate for increased research and financial support of forensic science as well as the introduction of empirical testing requirements to ensure the validity of outcomes.

World Science Forum 2017 Jordan issues Science for Peace Declaration
On behalf of the coordinating organizations responsible for delivering the World Science Forum Jordan, the concluding Science for Peace Declaration issued at the Dead Sea represents a global call for action to science and society to build a future that promises greater equality, security and opportunity for all, and in which science plays an increasingly prominent role as an enabler of fair and sustainable development.

PETA science group promotes animal-free science at society of toxicology conference
The PETA International Science Consortium Ltd. is presenting two posters on animal-free methods for testing inhalation toxicity at the 56th annual Society of Toxicology (SOT) meeting March 12 to 16, 2017, in Baltimore, Maryland.

Citizen Science in the Digital Age: Rhetoric, Science and Public Engagement
James Wynn's timely investigation highlights scientific studies grounded in publicly gathered data and probes the rhetoric these studies employ.

Science/Science Careers' survey ranks top biotech, pharma, and biopharma employers
The Science and Science Careers' 2016 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.

Three natural science professors win TJ Park Science Fellowship
Professor Jung-Min Kee (Department of Chemistry, UNIST), Professor Kyudong Choi (Department of Mathematical Sciences, UNIST), and Professor Kwanpyo Kim (Department of Physics, UNIST) are the recipients of the Cheong-Am (TJ Park) Science Fellowship of the year 2016.

Read More: Science News and Science Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to