Researchers decode genome of mosquito that spreads West Nile virus, encephalitis and elephantiasis

September 30, 2010

Chestnut Hill, Mass. (9/30/2010) - Scientists have sequenced the genome of the Southern house mosquito, providing new insights into the most diverse and widespread of three groups of disease-bearing mosquitoes and shedding new light on the transmission of mosquito-borne diseases such as malaria, encephalitis, West Nile virus and filariasis, international teams of researchers report in the upcoming edition of the journal Science.

Breeding in drains, cesspools and other polluted water bodies, Culex quinquefasciatus feeds on blood from birds, livestock and humans and transmits West Nile virus, St. Louis encephalitis and the microscopic roundworm that causes lymphatic filariasis, leading to 120 million infections and over 40 million cases of elephantiasis each year.

Mapping the genome of the Southern house mosquito effectively completes a platform for mosquito comparative genomics - a critical third piece of a genetic puzzle researchers have sought to solve in a global effort to contain the spread of infectious diseases transmitted by mosquitoes, according to co-authors of the first of two new reports.

Already, researchers have sequenced the genomes of two other mosquitoes, Aedes aegypti, which transmits yellow fever and dengue fever, and Anopheles gambiae, a species that carries malaria, a disease that infects 250 to 500 million people each year and kills nearly one million people annually, mostly young children in sub-Saharan Africa.

Culex differs from the two other arthropods in that its molecular "parts list" includes a staggering 18,883 protein-coding genes - that is 22 percent larger than for Ae. aegypti and 52 percent larger than for An. gambiae - with multiple gene family expansions, including those controlling smell and taste, immune responses and genes that attack toxic foreign compounds, the researchers discovered.

Greater understanding of these expanded gene sets could provide critical new insights into Culex and improve public health efforts. The mosquito's more complex genetic structure may have influenced evolution of Culex as an opportunistic feeder, able to detect and feed on birds, humans and livestock. This flexibility contributes to Culex's ability to transmit numerous disease-causing organisms - including West Nile virus, encephalitis viruses, filiarial worms and malaria parasites - to birds and humans, the researchers report.

"The consequent diversity in many different genes may be an important factor that led to the wide geographic distribution" of Culex, concludes a team of 69 co-authors of the genome report.

Armed with the genome sequence, researchers undertook the next step of uncovering the building blocks coded in the Culex genome that make it a deadly transmitter of disease, said Boston College DeLuca Professor of Biology Marc A.T. Muskavitch, a co-author of the first report and the senior author of the second, which was produced by an international team of 33 researchers.

"With the genome decoded, we have the building blocks. We can also determine which building blocks the mosquito uses to combat a pathogen and which genes the pathogen avoids when evading the defenses of the mosquito," said Muskavitch, who began collaborating with colleagues at the Broad Institute in 2007.

Researchers were able to analyze for the first time a set of 25 mosquito-pathogen interactions involving mosquito-borne viruses, filarial worms, malaria parasites and bacteria in all three mosquitoes. The analysis revealed common and distinct responses to these pathogens by the three and gave further credence to the theory that "mosquito-born pathogens have evolved to evade the innate immune responses in three vector mosquito species of major medical importance."

In the cases of Culex and Aedes aegypti, both are armed with hundreds of genes capable of triggering an immune response to the West Nile and encephalitis viruses. Instead only a few disease-fighting proteins respond in either mosquito, said Muskavitch, thereby allowing the viruses to take hold.

"Viruses fly under the radar of the mosquito's RNA interference infection response system - which should be defending it," he said. "Viruses have actually developed ways to evade the vast majority of the infection response systems of both Culex and Aedes aegypti."

Similar results were found when researchers examined the immune responses to filarial worms and bacteria.

Muskavitch said the genome advances are being shared with scientists around the globe as part of an international effort to bring researchers, doctors and public health experts the best information possible in order to combat the spread of these deadly and disfiguring diseases.

"Our goal is to determine how we can turn the building blocks of these mosquitoes against pathogens, in attempts to defeat those pathogens," Muskavitch said. "That is the scientific and public health significance of this new research."
-end-


Boston College

Related Malaria Articles from Brightsurf:

Clocking in with malaria parasites
Discovery of a malaria parasite's internal clock could lead to new treatment strategies.

Breakthrough in malaria research
An international scientific consortium led by the cell biologists Volker Heussler from the University of Bern and Oliver Billker from the UmeƄ University in Sweden has for the first time systematically investigated the genome of the malaria parasite Plasmodium throughout its life cycle in a large-scale experiment.

Scientists close in on malaria vaccine
Scientists have taken another big step forward towards developing a vaccine that's effective against the most severe forms of malaria.

New tool in fight against malaria
Modifying a class of molecules originally developed to treat the skin disease psoriasis could lead to a new malaria drug that is effective against malaria parasites resistant to currently available drugs.

Malaria expert warns of need for malaria drug to treat severe cases in US
The US each year sees more than 1,500 cases of malaria, and currently there is limited access to an intravenously administered (IV) drug needed for the more serious cases.

Monkey malaria breakthrough offers cure for relapsing malaria
A breakthrough in monkey malaria research by two University of Otago scientists could help scientists diagnose and treat a relapsing form of human malaria.

Getting to zero malaria cases in zanzibar
New research led by the Johns Hopkins Center for Communication Programs, Ifakara Health Institute and the Zanzibar Malaria Elimination Program suggests that a better understanding of human behavior at night -- when malaria mosquitoes are biting -- could be key to preventing lingering cases.

Widely used malaria treatment to prevent malaria in pregnant women
A global team of researchers, led by a research team at the Liverpool School of Tropical Medicine (LSTM), are calling for a review of drug-based strategies used to prevent malaria infections in pregnant women, in areas where there is widespread resistance to existing antimalarial medicines.

Protection against Malaria: A matter of balance
A balanced production of pro and anti-inflammatory cytokines at two years of age protects against clinical malaria in early childhood, according to a study led by ISGlobal, an institution supported by ''la Caixa'' Foundation.

The math of malaria
A new mathematical model for malaria shows how competition between parasite strains within a human host reduces the odds of drug resistance developing in a high-transmission setting.

Read More: Malaria News and Malaria Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.