Study first to tally biomass from oceanic plastic debris using visualization method

October 13, 2020

Trillions of plastic debris fragments are afloat at sea, creating the "perfect storm" for microbial colonization. Introduced more than 50 years ago, plastic substrates are a novel microbial habitat in the world's oceans. This "plastisphere" consists of a complex community comprised of bacterial, archaeal, and eukaryotic microorganisms and microscopic animals.

These unnatural additions to sea surface waters and the large quantity of cells and biomass carried by plastic debris has the potential to impact biodiversity, ecological functions and biogeochemical cycles within the ocean. Biofilm formation in the marine environment - a collective of one or more types of microorganisms that can grow on many different surfaces - is a complex process, involving many variables.

While several studies have surveyed microbial diversity and quantified specific members of these biofilm habitats, a new study is the first to holistically quantify total cell inventories under in situ conditions. This study is fundamentally different from others due to the relatively non-biased visualization methods used to arrive at a quantitative number for biomass, which is the first estimate of its kind.

Researchers from Florida Atlantic University's Harbor Branch Oceanographic Institute and Harriet L. Wilkes Honors College, in collaboration with Utrecht University, Netherlands, the University of Amsterdam, and The Royal Netherlands Institute for Sea Research (NIOZ), examined cell abundances, size, cellular carbon mass, and how photosynthetic cells differ on polymeric and glass substrates over time. They investigated nanoparticle generation from plastic such as polystyrene, which is known to disintegrate into nanoparticles in sunlight and ultraviolet radiation, and how this might disrupt microalgae.

Results of the study, published in the ISME Journal, a monthly publication of the International Society for Microbial Ecology, reveal that by measuring the average microbial biomass carrying capacity of different plastic polymers and, by extension, plastic marine debris in the global ocean, conservative estimates suggest that about 1 percent of microbial cells in the ocean surface microlayer inhabit plastic debris globally. This mass of cells would not exist if plastic debris was not in the ocean, and therefore, represents a disruption of the proportions of native flora in that habitat.

"In the open ocean, nutrients are limiting. Just like we need to put fertilizer on a garden, microorganisms in the ocean are limited by nitrogen, iron or phosphorous depending upon where they are -- except in the open ocean, there is typically no fertilizer, so something has to die for another organism to live," said Tracy Mincer, Ph.D., lead author and an assistant professor of biology/bio-geochemistry at FAU's Harbor Branch and Wilkes Honors College. "With the advantage of a surface, which concentrates nutrients, organisms colonizing plastics in the ocean are taking up those limiting nutrients that normally would have been consumed or out-competed by free-living microbes. So essentially, these microbes on plastics are taking habitat space away and represent the beginning of a regime shift for these habitats."

Using confocal laser scanning microscopy with sophisticated imaging software, researchers directly obtained data ranging from cell counts, size and the characterization of microbial morphotypes to complete three-dimensional constructs. They tested a range of chemically distinct substrates that included polypropylene, polystyrene, polyethylene and glass. Polypropylene is used by the automotive industry, for consumer goods such as packaging, industrial applications and the furniture market; polystyrene is used to make clear products like food packing or laboratory equipment; and polyethylene is the most widely used plastic in the world ranging from products such as clear food wrap to shopping bags to detergent bottles.

Data from the confocal laser scanning microscopy showed that early biofilms displayed a high proportion of diatoms (unicellular eukaryotic microalgae that have cell walls made of glass). These diatoms could play a key role in the sinking of plastic debris. Unexpectedly, plastic substrates appeared to reduce the growth of photosynthetic cells after eight weeks compared to glass.

"The quantification of cell numbers and microbial biomass on plastic marine debris is crucial for understanding the implications of plastic marine debris on oceanic ecosystems," said Shiye Zhao, Ph.D., first author and a post-doctoral fellow at FAU's Harbor Branch. "Future efforts should focus on how this biomass fluctuates with season and latitude and its potential to perturb the flux of nutrients in the upper layers of the ocean."
About Harbor Branch Oceanographic Institute:

Founded in 1971, Harbor Branch Oceanographic Institute at Florida Atlantic University is a research community of marine scientists, engineers, educators and other professionals focused on Ocean Science for a Better World. The institute drives innovation in ocean engineering, at-sea operations, drug discovery and biotechnology from the oceans, coastal ecology and conservation, marine mammal research and conservation, aquaculture, ocean observing systems and marine education. For more information, visit

About Florida Atlantic University:

Florida Atlantic University, established in 1961, officially opened its doors in 1964 as the fifth public university in Florida. Today, the University, with an annual economic impact of $6.3 billion, serves more than 30,000 undergraduate and graduate students at sites throughout its six-county service region in southeast Florida. FAU's world-class teaching and research faculty serves students through 10 colleges: the Dorothy F. Schmidt College of Arts and Letters, the College of Business, the College for Design and Social Inquiry, the College of Education, the College of Engineering and Computer Science, the Graduate College, the Harriet L. Wilkes Honors College, the Charles E. Schmidt College of Medicine, the Christine E. Lynn College of Nursing and the Charles E. Schmidt College of Science. FAU is ranked as a High Research Activity institution by the Carnegie Foundation for the Advancement of Teaching. The University is placing special focus on the rapid development of critical areas that form the basis of its strategic plan: Healthy aging, biotech, coastal and marine issues, neuroscience, regenerative medicine, informatics, lifespan and the environment. These areas provide opportunities for faculty and students to build upon FAU's existing strengths in research and scholarship. For more information, visit

Florida Atlantic University

Related Biomass Articles from Brightsurf:

Bound for the EU, American-made biomass checks the right boxes
A first-of-its-kind study published in the journal Scientific Reports finds that wood produced in the southeastern United States for the EU's renewable energy needs has a net positive effect on US forests--but that future industry expansion could warrant more research.

The highest heat-resistant plastic ever is developed from biomass
The use of biomass-derived plastics is one of the prime concerns to establish a sustainable society, which is incorporated as one of the Sustainable Development Goals.

Laser technology measures biomass in world's largest trees
Laser technology has been used to measure the volume and biomass of giant Californian redwood trees for the first time, records a new study by UCL researchers.

Inducing plasma in biomass could make biogas easier to produce
Producing biogas from the bacterial breakdown of biomass presents options for a greener energy future, but the complex composition of biomass comes with challenges.

Microbes working together multiply biomass conversion possibilities
Non-edible plants are a promising alternative to crude oil, but their heterogenous composition can be a challenge to producing high yields of useful products.

Evergreen idea turns biomass DNA into degradable materials
A Cornell-led collaboration is turning DNA from organic matter -- such as onions, fish and algae -- into biodegradable gels and plastics.

Upgrading biomass with selective surface-modified catalysts
Loading single platinum atoms on titanium dioxide promotes the conversion of a plant derivative into a potential biofuel.

A novel biofuel system for hydrogen production from biomass
A recent study, affiliated with South Korea's Ulsan National Institute of Science and Technology (UNIST) has presented a new biofuel system that uses lignin found in biomass for the production of hydrogen.

Biomass fuels can significantly mitigate global warming
'Every crop we tested had a very significant mitigation capacity despite being grown on very different soils and under natural climate variability,' says Dr.

Traditional biomass stoves shown to cause lung inflammation
Traditional stoves that burn biomass materials and are not properly ventilated, which are widely used in developing nations where cooking is done indoors, have been shown to significantly increase indoor levels of harmful PM2.5 (miniscule atmospheric particulates) and carbon monoxide (CO) and to stimulate biological processes that cause lung inflammation and may lead to chronic obstructive pulmonary disease (COPD), according to new research published online in the Annals of the American Thoracic Society.

Read More: Biomass News and Biomass Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to