Nav: Home

Imaging electric charge propagating along microbial nanowires

October 19, 2014

AMHERST, Mass. ¬- The claim by microbiologist Derek Lovley and colleagues at the University of Massachusetts Amherst that the microbe Geobacter produces tiny electrical wires, called microbial nanowires, has been mired in controversy for a decade, but the researchers say a new collaborative study provides stronger evidence than ever to support their claims.

UMass Amherst physicists working with Lovley and colleagues report in the current issue of Nature Nanotechnology that they've used a new imaging technique, electrostatic force microscopy (EFM), to resolve the biological debate with evidence from physics, showing that electric charges do indeed propagate along microbial nanowires just as they do in carbon nanotubes, a highly conductive man-made material.

Physicists Nikhil Malvankar and Sibel Ebru Yalcin, with physics professor Mark Tuominen, confirmed the discovery using EFM, a technique that can show how electrons move through materials. "When we injected electrons at one spot in the microbial nanowires, the whole filament lit up as the electrons propagated through the nanowire," says Malvankar.

Yalcin, now at Pacific Northwest National Lab, adds, "This is the same response that you would see in a carbon nanotube or other highly conductive synthetic nanofilaments. Even the charge densities are comparable. This is the first time that EFM has been applied to biological proteins. It offers many new opportunities in biology."

Lovley says the ability of electric current to flow through microbial nanowires has important environmental and practical implications. "Microbial species electrically communicate through these wires, sharing energy in important processes such as the conversion of wastes to methane gas. The nanowires permit Geobacter to live on iron and other metals in the soil, significantly changing soil chemistry and playing an important role in environmental cleanup. Microbial nanowires are also key components in the ability of Geobacter to produce electricity, a novel capability that is being adapted to engineer microbial sensors and biological computing devices."

He acknowledges that there has been substantial skepticism that Geobacter's nanowires, which are protein filaments, could conduct electrons like a wire, a phenomenon known as metallic-like conductivity. "Skepticism is good in science, it makes you work harder to evaluate whether what you are proposing is correct," Lovley points out. "It's always easier to understand something if you can see it. Drs. Malvankar and Yalcin came up with a way to visualize charge propagation along the nanowires that is so elegant even a biologist like me can easily grasp the mechanism."

Biologists have known for years that in biological materials, electrons typically move by hopping along discrete biochemical stepping-stones that can hold the individual electrons. By contrast, electrons in microbial nanowires are delocalized, not associated with just one molecule. This is known as metallic-like conductivity because the electrons are conducted in a manner similar to a copper wire.

Malvankar, who provided the first evidence for the metallic-like conductivity of the microbial nanowires in Lovley and Tuominen's labs in 2011, says, "Metallic-like conductivity of the microbial nanowires seemed clear from how it changed with different temperature or pH, but there were still many doubters, especially among biologists."

To add more support to their hypothesis, Lovley's lab genetically altered the structure of the nanowires, removing the aromatic amino acids that provide the delocalized electrons necessary for metallic-like conductivity, winning over more skeptics. But EFM provides the final, key evidence, Malvankar says.

"Our imaging shows that charges flow along the microbial nanowires even though they are proteins, still in their native state attached to the cells. Seeing is believing. To be able to visualize the charge propagation in the nanowires at a molecular level is very satisfying. I expect this technique to have an especially important future impact on the many areas where physics and biology intersect." he adds.

Tuominen says, "This discovery not only puts forward an important new principle in biology but also in materials science. Natural amino acids, when arranged correctly, can propagate charges similar to molecular conductors such as carbon nanotubes. It opens exciting opportunities for protein-based nanoelectronics that was not possible before."

Lovley and colleagues' microbial nanowires are a potential "green" electronics component, made from renewable, non-toxic materials. They also represent a new part in the growing field of synthetic biology, he says. "Now that we understand better how the nanowires work, and have demonstrated that they can be genetically manipulated, engineering 'electric microbes' for a diversity of applications seems possible."

One application currently being developed is making Geobacter into electronic sensors to detect environmental contaminants. Another is Geobacter-based microbiological computers. This work was supported by the Office of Naval Research, the U.S. Department of Energy and the National Science Foundation.
-end-


University of Massachusetts at Amherst

Related Carbon Nanotubes Articles:

Why modified carbon nanotubes can help the reproducibility problem
Scientists at Tokyo Institute of Technology (Tokyo Tech) conducted an in-depth study on how carbon nanotubes with oxygen-containing groups can be used to greatly enhance the performance of perovskite solar cells.
Tensile strength of carbon nanotubes depends on their chiral structures
Single-walled carbon nanotubes should theoretically be extremely strong, but it remains unclear why their experimental tensile strengths are lower and vary among nanotubes.
New study reveals carbon nanotubes measurement possible for the first time
Swansea University scientists report an entirely new approach to manipulation of carbon nanotubes that allows physical measurements to be made on carbon nanotubes that have previously only been possible by theoretical computation.
Neural networks will help manufacture carbon nanotubes
A team of scientists from Skoltech's Laboratory of Nanomaterials proposed a neural-network-based method for monitoring the growth of carbon nanotubes, preparing the ground for a new generation of sophisticated electronic devices.
Efficient, interconnected, stable: New carbon nanotubes to grow neurons
Carbon nanotubes able to take on the desired shapes thanks to a special chemical treatment, called crosslinking and, at the same time, able to function as substrata for the growth of nerve cells, finely tuning their growth and activity.
OU Researcher determines catalytic active sites using carbon nanotubes
Catalytic research led by University of Oklahoma researcher Steven Crossley has developed a new and more definitive way to determine the active site in a complex catalyst.
Carbon in color: First-ever colored thin films of nanotubes created
A method developed at Aalto University, Finland, can produce large quantities of pristine single-walled carbon nanotubes in select shades of the rainbow.
FEFU scientists reported on toxicity of carbon and silicon nanotubes and carbon nanofibers
Nanoparticles with a wide range of applying, including medicine, damage cells of microalgae Heterosigma akashivo badly.
Making carbon nanotubes as usable as common plastics
By using an inexpensive, already mass produced, simple solvent called cresol, Northwestern University's Jiaxing Huang has discovered a way to make disperse carbon nanotubes at unprecedentedly high concentrations without the need for additives or harsh chemical reactions to modify the nanotubes.
Carbon nanotubes devices may have a limit to how 'nano' they can be
Carbon nanotubes bound for electronics not only need to be as clean as possible to maximize their utility in next-generation nanoscale devices, but contact effects may limit how small a nano device can be, according to researchers at the Energy Safety Research Institute (ESRI) at Swansea University in collaboration with researchers at Rice University.
More Carbon Nanotubes News and Carbon Nanotubes Current Events

Best Science Podcasts 2019

We have hand picked the best science podcasts for 2019. Sit back and enjoy new science podcasts updated daily from your favorite science news services and scientists.
Now Playing: TED Radio Hour

Rethinking Anger
Anger is universal and complex: it can be quiet, festering, justified, vengeful, and destructive. This hour, TED speakers explore the many sides of anger, why we need it, and who's allowed to feel it. Guests include psychologists Ryan Martin and Russell Kolts, writer Soraya Chemaly, former talk radio host Lisa Fritsch, and business professor Dan Moshavi.
Now Playing: Science for the People

#538 Nobels and Astrophysics
This week we start with this year's physics Nobel Prize awarded to Jim Peebles, Michel Mayor, and Didier Queloz and finish with a discussion of the Nobel Prizes as a way to award and highlight important science. Are they still relevant? When science breakthroughs are built on the backs of hundreds -- and sometimes thousands -- of people's hard work, how do you pick just three to highlight? Join host Rachelle Saunders and astrophysicist, author, and science communicator Ethan Siegel for their chat about astrophysics and Nobel Prizes.