Nav: Home

3D printing, bioinks create implantable blood vessels

October 22, 2019

WASHINGTON, D.C., October 22, 2019 -- A biomimetic blood vessel was fabricated using a modified 3D cell printing technique and bioinks, which were formulated from smooth muscle cells from a human aorta and endothelial cells from an umbilical vein. The result is a fully functional blood vessel with a dual-layer architecture that outperforms existing engineered tissue and brings 3D-printed blood vessels several fundamental steps closer to clinical use.

The engineered blood vessels were grafted as abdominal aortas into six rats. Over the next several weeks, scientists observed a transformation in which the rat's fibroblasts formed a layer of connective tissue on the surface of the implant to integrate the fabricated vessel graft as part of the existing, living tissue. The results, published in Applied Physics Reviews, from AIP Publishing, include details on the triple-coaxial 3D printing technology they developed and their analysis of the unique architecture, physical strengths and biological activity of the engineered tissue.

"The artificial blood vessel is an essential tool to save patients suffering from cardiovascular disease," author Ge Gao said. "There are products in clinical use made from polymers, but they don't have living cells and vascular functions. We wanted to tissue-engineer a living, functional blood vessel graft."

Prior attempts to construct small-diameter blood vessels have yielded blood vessels that are fragile and prone to blockage. They often use a stripped-down version of extracellular material, such as collagen-based bioinks. In contrast, material from a native blood vessel contains collagen plus a collection of diverse biomolecules that provide a favorable microenvironment for vascular cell growth.

Using these native-materials-based bioinks preserves the natural complexity of the blood vessel and accelerates the generation of functional vascular tissues, so they have enhanced strength and anti-thrombosis functions.

After fabrication, the printed blood vessel was matured in a lab that was designed to tune the vessel's biological and physical characteristics to precise specifications of wall thickness, cellular alignment, burst pressure, tensile strength, and its ability to contract, mimicking natural blood vessel function.

The authors plan to continue to develop processes to increase the strength of the blood vessels closer to that of human coronary arteries. They also plan to perform long-term evaluation of vascular grafts, observing what happens as they continue to develop in place and become real tissue in the implanted environment.
-end-
The article, "Tissue-engineering of vascular grafts containing endothelium and smooth-muscle using triple-coaxial cell printing," is authored by Ge Gao, Hyeok Kim, Byoung Soo Kim, Jeong Sik Kong, Jae Yeon Lee, Bong Woo Park, Su Hun Chae, Jisoo Kim, Kiwon Ban, Jinah Jang, Hun-Jun Park and Dong-Woo Cho. The article will appear in the journal Applied Physics Reviews on October 22, 2019 (DOI: 10.1063/1.5099306). After that date, it can be accessed at https://aip.scitation.org/doi/10.1063/1.5099306.

ABOUT THE JOURNAL

Applied Physics Reviews features articles on significant and current topics in experimental or theoretical research in applied physics, or in applications of physics to other branches of science and engineering. The journal publishes both original research on pioneering studies of broad interest to the applied physics community, and reviews on established or emerging areas of applied physics. See https://aip.scitation.org/journal/are.

American Institute of Physics

Related Blood Vessel Articles:

Engineered living-cell blood vessel provides new insights to progeria
Scientists have developed the most advanced disease model for blood vessels to date and used it to discover a unique role of the endothelium in Hutchinson-Gilford Progeria Syndrome.
Improving blood vessel health in the brain may help combat Alzheimer's
Researchers have found that very slow spontaneous blood vessel pulsations drive the clearance of substances from the brain, indicating that targeting and improving this process may help to prevent or treat amyloid-beta accumulation.
Eating cheese may offset blood vessel damage from salt
Antioxidants naturally found in cheese may help protect blood vessels from damage from high levels of salt in the diet, according to a new Penn State study.
Sunscreen use could lead to better blood vessel health
A new study suggests that sunscreen protects the skin's blood vessel function from harmful ultraviolet radiation (UVR) exposure by protecting dilation of the blood vessels.
Blood runs deep: Lab blood vessel model sheds light on angiogenesis
Researchers at the University of Tokyo and at CNRS in France revealed the importance of the molecule EGFL7 for angiogenesis and endothelial integrity using an artificially created blood vessel model called a microvessel-on-a-chip.
Discovery of blood vessel system in bones
A network of very fine blood vessels that connects bone marrow directly with the blood supply of the periosteum that was previously overlooked has now been discovered by Dr.
Breakthrough in blood vessel engineering
Growing functional blood vessel networks is no easy task. Previously, other groups have made networks that span millimeters in size.
Just one energy drink may hurt blood vessel function
Young, healthy adults experienced notably diminished blood vessel function soon after consuming one energy drink, according to preliminary research from a small study to be presented in Chicago at the American Heart Association's Scientific Sessions 2018, a premier global exchange of the latest advances in cardiovascular science for researchers and clinicians.
New targets found to reduce blood vessel damage in diabetes
In diabetes, both the tightly woven endothelial cells that line our blood vessels and the powerhouses that drive those cells start to come apart as early steps in the destruction of our vasculature.
The vessel not taken: Understanding disproportionate blood flow
Each time a blood vessel splits into smaller vessels, red blood cells (RBCs) are presented with the same decision: Take the left capillary or the right.
More Blood Vessel News and Blood Vessel Current Events

Trending Science News

Current Coronavirus (COVID-19) News

Top Science Podcasts

We have hand picked the top science podcasts of 2020.
Now Playing: TED Radio Hour

Climate Mindset
In the past few months, human beings have come together to fight a global threat. This hour, TED speakers explore how our response can be the catalyst to fight another global crisis: climate change. Guests include political strategist Tom Rivett-Carnac, diplomat Christiana Figueres, climate justice activist Xiye Bastida, and writer, illustrator, and artist Oliver Jeffers.
Now Playing: Science for the People

#562 Superbug to Bedside
By now we're all good and scared about antibiotic resistance, one of the many things coming to get us all. But there's good news, sort of. News antibiotics are coming out! How do they get tested? What does that kind of a trial look like and how does it happen? Host Bethany Brookeshire talks with Matt McCarthy, author of "Superbugs: The Race to Stop an Epidemic", about the ins and outs of testing a new antibiotic in the hospital.
Now Playing: Radiolab

Speedy Beet
There are few musical moments more well-worn than the first four notes of Beethoven's Fifth Symphony. But in this short, we find out that Beethoven might have made a last-ditch effort to keep his music from ever feeling familiar, to keep pushing his listeners to a kind of psychological limit. Big thanks to our Brooklyn Philharmonic musicians: Deborah Buck and Suzy Perelman on violin, Arash Amini on cello, and Ah Ling Neu on viola. And check out The First Four Notes, Matthew Guerrieri's book on Beethoven's Fifth. Support Radiolab today at Radiolab.org/donate.