News tips from the Journal of Neuroscience

October 23, 2007

1. GIPc and Extracellular NMDA Receptors

Zhaohong Yi, Ronald S. Petralia, Zhanyan Fu, Catherine Croft Swanwick, Ya-Xian Wang, Kate Prybylowski, Nathalie Sans, Stefano Vicini, and Robert J. Wenthold

The usual textbook view is that synaptic receptors, particularly NMDA receptors, are stably anchored at synapses, whereas extra synaptic receptors are wandering freely in the hinterlands. Not so simple, say Yi et al. The authors used a yeast two-hybrid screen to turn up an interaction between the NMDA receptor subunit NR2B and a novel protein with the well-chosen name GIPC (GAIP-interacting protein, C terminus). The association required the GIPC PDZ (PSD-95/Dlg/ZO-1) domain and the NR2B PDZ binding domain. A chimera consisting of the single transmembrane domain protein Tac and the distal cytoplasmic region of NR2B, TacNR2B, colocalized with GIPC on the cell surface and in the cytoplasm. The expression level of GIPC had no effect on total NMDA receptor expression, but surface expression in hippocampal neurons fluctuated with GIPC expression. GIPC colocalized with NR2B but was excluded from synapses, suggesting that GIPC contributes to trafficking and stabilization of extrasynaptic NMDA receptors.

2. Deadly Ganglionic a7 nicotinic Receptors

Martin Hruska and Rae Nishi

Culling of select neurons is a fundamental characteristic of brain development, but the target-derived and intrinsic factors that determine cell fate are still being mapped. This week, Hruska and Nishi propose a cell-autonomous action of a7 nicotinic acetylcholine receptors (nAChRs) in the developmental death of avian ciliary ganglion neurons. Half of these neurons die between embryonic day 8 (E8) and E14. Because nAChR antagonists prevent this cell death, the authors examined the site and mechanism of antagonist-mediated rescue. Labeling of a7 nAChRs at E8 with a-bungarotoxin (a btx)-Alexa 488 revealed heterogeneity in surface receptor density of these calcium-permeable channels. Nicotine induced greater calcium influx in receptor-dense neurons, which was blocked by nicotinic antagonists. The intracellular calcium signal was reduced by expression of abtx tethered to the membrane by a glycosylphosphatidylinositol linkage similar to externally applied toxin. Moreover, toxin expression rescued neurons from impending death.

3. κ Opiates and Aversion to Stress

Michael R. Bruchas, Benjamin B. Land, Megumi Aita, Mei Xu, Sabiha Barot, Shuang Li, and Charles Chavkin

Chronic stress activates the endogenous opioid system and produces dysphoria, an umbrella term that includes depression and associated behaviors. This behavior response may be mediated in part by the dynorphin-κ-opioid system. In this week's Journal, Bruchas et al. examined the link between activation of the G-protein-coupled κ-opioideceptor (KOR) and downstream activation of the mitogen-activated protein kinase (MAPK) signaling system, specifically the stress kinase p38. In mice subjected to a repeated swim-stress test, KOR and p38 were activated in inhibitory neurons of the nucleus accumbens, cortex, and hippocampus, visualized by phosphospecific antibodies. The p38 inhibitor SB203580 alleviated the immobility resulting from repeated stress, as well as another behavior associated with KOR activation, conditioned place aversion. Activation of p38 depended on phosphorylation of KOR by the G-protein receptor kinase GRK3.

4. BDNF Depletion as a Model of Huntington's Disease

Andrew D. Strand, Zachary C. Baquet, Aaron K. Aragaki, Peter Holmans, Lichuan Yang, Carine Cleren, M. Flint Beal, Lesley Jones, Charles Kooperberg, James M. Olson, and Kevin R. Jones

Fourteen years after discovery of the disease gene, huntingtin, the pathophysiology of Huntington's disease (HD) remains an enigma. This week Strand et al. decided to compare striatal gene expression in mouse models of HD with human HD. The genetic model, the R6/2 mouse, expresses a fragment of huntingtin (htt) containing a long CAG repeat. Other mice, such as those treated with 3NP, an inhibitor of mitochondrial electron transport that causes striatal degeneration, were also examined. Finally, the authors tested mice with conditional deletion of brain-derived neurotrophic factor (BDNF) from cortical neurons (Emx- BDNF) or with a heterozygous nullBDNF mutation. Surprisingly, BDNF-deficient mice showed the best concordance with human HD. Because corticostriatal axons are the major source of striatal BDNF, the data suggest that dysfunction in the cortex could contribute to striatal degeneration in HD. BDNF depletion is a plausible mechanism because mutant htt allows nuclear translocation of the neuronal suppressor REST, thus suppressing BDNF transcription. A testable hypothesis it seems.

Society for Neuroscience

Related Protein Articles from Brightsurf:

The protein dress of a neuron
New method marks proteins and reveals the receptors in which neurons are dressed

Memory protein
When UC Santa Barbara materials scientist Omar Saleh and graduate student Ian Morgan sought to understand the mechanical behaviors of disordered proteins in the lab, they expected that after being stretched, one particular model protein would snap back instantaneously, like a rubber band.

Diets high in protein, particularly plant protein, linked to lower risk of death
Diets high in protein, particularly plant protein, are associated with a lower risk of death from any cause, finds an analysis of the latest evidence published by The BMJ today.

A new understanding of protein movement
A team of UD engineers has uncovered the role of surface diffusion in protein transport, which could aid biopharmaceutical processing.

A new biotinylation enzyme for analyzing protein-protein interactions
Proteins play roles by interacting with various other proteins. Therefore, interaction analysis is an indispensable technique for studying the function of proteins.

Substituting the next-best protein
Children born with Duchenne muscular dystrophy have a mutation in the X-chromosome gene that would normally code for dystrophin, a protein that provides structural integrity to skeletal muscles.

A direct protein-to-protein binding couples cell survival to cell proliferation
The regulators of apoptosis watch over cell replication and the decision to enter the cell cycle.

A protein that controls inflammation
A study by the research team of Prof. Geert van Loo (VIB-UGent Center for Inflammation Research) has unraveled a critical molecular mechanism behind autoimmune and inflammatory diseases such as rheumatoid arthritis, Crohn's disease, and psoriasis.

Resurrecting ancient protein partners reveals origin of protein regulation
After reconstructing the ancient forms of two cellular proteins, scientists discovered the earliest known instance of a complex form of protein regulation.

Sensing protein wellbeing
The folding state of the proteins in live cells often reflect the cell's general health.

Read More: Protein News and Protein Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to