Crystal structure of SARS-CoV-2 papain-like protease

October 27, 2020

The pandemic of coronavirus disease 2019 (COVID-19) is changing the world like never before. This crisis is unlikely contained in the absence of effective therapeutics or vaccine. The papain-like protease (PLpro) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays essential roles in virus replication and immune evasion, presenting a promising drug target.

The authors of this paper determined two important structures of SARS-CoV-2 PLpro protease. The unliganded structure has novel crystal packing, high solvent content and reasonable resolution; thus, it offers a good foundation for fragment-based screening targeting the enzyme. The GRL0617 bound structure provides valuable insight into the inhibition mechanism at atomic level. Given that GRL0617 is one of the most promising inhibitors of CoV PLpro, the authors findings will aid further optimization of the inhibitor, which may contribute to speed up therapeutic development of COVID-19.
-end-
Article reference: Xiaopan Gao, Bo Qin, Pu Chen, Kaixiang Zhu, Pengjiao Hou, Justyna Aleksandra Wojdyla, Meitian Wang, Sheng Cui, Crystal structure of SARS-CoV-2 papain-like protease, Acta Pharmaceutica Sinica B, 2020, ISSN 2211-3835, https://doi.org/10.1016/j.apsb.2020.08.014

Keywords: SARS-CoV-2, PLpro, Proteinase inhibitor, Crystal structure, Antiviral drug, Drug design

The Journal of the Institute of Materia Medica, the Chinese Academy of Medical Sciences and the Chinese Pharmaceutical Association.

Acta Pharmaceutica Sinica B (APSB) is a monthly journal, in English, which publishes significant original research articles, rapid communications and high quality reviews of recent advances in all areas of pharmaceutical sciences -- including pharmacology, pharmaceutics, medicinal chemistry, natural products, pharmacognosy, pharmaceutical analysis and pharmacokinetics.

For more information please visit https://www.journals.elsevier.com/acta-pharmaceutica-sinica-b/

Editorial Board: https://www.journals.elsevier.com/acta-pharmaceutica-sinica-b/editorial-board

APSB is available on ScienceDirect (https://www.sciencedirect.com/journal/acta-pharmaceutica-sinica-b).

Submissions to APSB may be made using Editorial Manager® (https://www.editorialmanager.com/apsb/default.aspx).

CiteScore: 10.5

Impact Factor: 7.097

5-Year Impact Factor: 7.865

Source Normalized Impact per Paper (SNIP): 2.210

SCImago Journal Rank (SJR): 1.792ISSN 2211-3835

Compuscript Ltd

Related Virus Replication Articles from Brightsurf:

Researchers develop virus live stream to study virus infection
Researchers from the Hubrecht Institute and Utrecht University developed an advanced technique that makes it possible to monitor a virus infection live.

Study reveals strategy to create COVID-19 drugs to inhibit virus's entry and replication
A new study offers insight into designing antiviral drugs against COVID-19 by showing that some existing compounds can inhibit both the main protease (Mpro), a key viral protein required for SARS-CoV-2 replication inside human cells, and the lysosomal protease cathepsin L, a human protein important for viral entry into host cells.

Exercise hormone may modulate genes associated with replication of novel coronavirus
Brazilian researchers observed that in uninfected adipocytes, the hormone irisin altered the expression of genes that regulate ACE-2, which encodes a protein to which the virus binds in order to invade human cells.

Lab-made virus mimics COVID-19 virus
Researchers at Washington University School of Medicine in St. Louis have created a virus in the lab that infects cells and interacts with antibodies just like the COVID-19 virus, but lacks the ability to cause severe disease.

Dynamics of DNA replication revealed at the nanoscale
Using super-resolution technology a University of Technology Sydney led team has directly visualised the process of DNA replication in single human cells.

Two anti-inflammatory drugs found that inhibit the replication of the COVID-19 virus
Researchers at the URV have used computer techniques to analyse whether 6,466 drugs authorized by various drug agencies for both human and veterinary use could be used to inhibit the M-pro enzyme.

Stitching together the structure of the DNA replication toolbelt
Structural details of an enzyme complex shed light on DNA replication.

New details on how a viral protein puts the brakes on virus replication
Researchers used computational chemistry, biochemistry and virology to uncover new information on how viruses such as West Nile, dengue and Zika replicate.

How cells relieve DNA replication stress
IBS scientists revealed that ATAD5 actively deals with replication stress, in addition to its known function to prevent such stressful situations.

Scientists unwind mystery behind DNA replication
The molecules of life are twisted. But how those familiar strands in DNA's double helix manage to replicate without being tangled up has been hard to decipher.

Read More: Virus Replication News and Virus Replication Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.