Powerful integration of lipid metabolic profiling with gene expression analysis

November 05, 2007

A recently published research article in the "Journal of Proteome Research", authored by researchers from the Nestlé Reserarch Center, Genomatix Software GmbH, Rosetta Inpharmatics LLC, CXR Biosciences Ltd, the Cancer Research UK Molecular Pharmacology Unit demonstrates the synergisms and enhanced analytic power of the combination of thorough metabolic profiling with the unique and proprietary microarray analysis methods of Genomatix Software GmbH.

The study elucidated the effects on Mouse lipid metabolism by the disruption of hepatic Cytochrome P450 reductase (POR) in a POR deficient knock-out mouse model. It clearly could demonstrate that though gene expression and lipid metabolism in extrahepatic tissues being sensitive to hepatic POR functionality, the lipidome in general is only minimally affected. Analysis of regulatory pathways and networks, observed expression changes and lipid profiling led to the conclusion that POR can be considered an enzyme critical for the proper functioning of lipid mobilization and metabolism predominantly within the mouse liver with only minor effects on lipid metabolism in the biological system at large.

The concordance between the two analytical platforms (Genomatix transcriptomic vs lipidomic) in the liver was excellent, and serves as a demonstration for the valid approach for generating novel hypüotheses to unravel protein function that cannot be accomplished with as much confiodence using either platform individually.

"I am very excited about the possibility to overlay metabolic profiles with expression data and pathway information. We see the strategies applied in this study as a prototype for further analyzes bringing together metabolomics and expression analysis in a multiple lines of evidence manner. " says Dr. Martin Seifert, co-author of the study and Vice President Microarray Business and Collaborative Research at Genomatix.
-end-
The study is published here:

Mutch DM, Klocke B, Morrison P, Murray CA, Henderson CJ, Seifert M, Williamson G (2007)
The Disruption of Hepatic Cytochrome P450 Reductase Alters Mouse Lipid Metabolism.
J Proteome Res. 6, 3976-3984
[PUBMED: 17722906]

About Genomatix:

Genomatix is a pioneer and leader in the analysis and understanding of eukaryotic gene regulation. Core competences at Genomatix are annotation driven microarray analysis, ChIP on chip, regulatory network and pathway mining, and promoter analysis on sequence level. Genomatix has published more than 170 peer reviewed scientific papers that have been cited in more than 4,000 papers. Over 35,000 researchers worldwide currently apply Genomatix tools and databases. More information is available from http://www.genomatix.de.

Genomatix Software GmbH

Related Lipid Metabolism Articles from Brightsurf:

Lipid-Oligonucleotides (LONs) --- Promising materials for bioapplications
Lipid-oligonucleotides (LONs) are promising biological materials with special amphiphilic structures and unique functionalities of two moieties, contributing to different bioapplications (from biosensors to biomedicines).

Altered lipid metabolism following childbirth predicts later diabetes risk
Scientists have found that disruptions to the metabolism of lipids occur after childbirth in women with gestational diabetes who go on to develop type 2 diabetes.

Camelina sativa oil and fatty fish have positive effects on lipid metabolism
Camelina sativa oil and fatty fish are rich in polyunsaturated omega-3 fatty acids, but their health benefits seem to differ.

Computer modeling reveals behavior of individual lipid molecules
Lipids are essential building blocks of cell membranes, which control the exchange of substances and energy between a cell and its environment.

Lipid metabolism controls brain development
A lipid metabolism enzyme controls brain stem cell activity and lifelong brain development.

Surveying the lipid landscape
Software LipidCreator enables researchers to characterize 60 lipid classes in cells with mass spectrometry.

The lipid code
So far, it has been difficult to analyze the functions of lipid molecules in living cells.

Lipid gradient that keeps your eyes wet
New understandings of how lipids function within tears could lead to better drugs for treating dry eye disease.

Lipid helps heal the eye's frontline protection
A species of a lipid that naturally helps skin injuries heal appears to also aid repair of common corneal injuries, even when other conditions, like diabetes, make healing difficult, scientists report.

Reduced stress changes profile of various lipid compounds
Reduced stress is linked to changes in the profile of plasma metabolites, a new study from the University of Eastern Finland shows.

Read More: Lipid Metabolism News and Lipid Metabolism Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.