Solution of the high-resolution crystal structure of stress proteins from Staphylococcus

November 06, 2019

Project leader, Head of Structural Biology Lab Konstantin Usachev explains, "One of the main factors favoring a microorganism's survival in extreme conditions is preserving ribosomes - a macromolecular complex comprising RNA and proteins. For this purpose, a cell synthesizes special proteins which may stop translating ribosomes until the stress is over. One of such proteins is hibernation promoting factor (HPF) which transfers ribosomes to the 'hibernation state'."

Staphylococcus aureus is one of the most dangerous bacteria for humans, a pathogen causing many nosocomial infections. The danger is both in the staph's virulence and antibiotic resistance. There are currently no specific anti-staphylococcus antibiotics. That's why it's so important to seek new targets for antibiotic therapy in the bacteria.

"Using high-resolution (1,6 Å) X-ray structure analysis, we solved the structure of the domain of HPF from Staphylococcus aureus, which regulates the dimerization of ribosomes and keeps in their 'hibernation state'. Based on the obtained information, we found the key amino acid residues which provide for the functioning of the protein and used genetic engineering to produce mutated forms of this protein by single amino acid residues substitutions. After that, the mutated forms were analyzed for their capabilities of ribosome hibernation, and we found five residues essential for the functioning of the protein. Our results open the way to exploring new compounds that could bond with this protein areas and decrease the vitality of Staphylococcus aureus under stress," adds Dr. Usachev.

This was the first protein structure solved by X-ray analysis implemented with the new XtaLab Synergy S diffractometer; it was installed at the Structural Biology Lab this past May.

"Our new diffractometer has a sensitive detector, which allows analyzing the structure of large biomolecules in a monocrystal form, such as proteins and nucleic acids," concludes Dr. Usachev.
-end-
The Structural Biology Lab currently cooperates with the Chemoinformatics and Molecular Modelling Lab in searching the databases for new compounds which can bind with the Staph stress proteins.

Kazan Federal University

Related Stress Articles from Brightsurf:

Stress-free gel
Researchers at The University of Tokyo studied a new mechanism of gelation using colloidal particles.

Early life stress is associated with youth-onset depression for some types of stress but not others
Examining the association between eight different types of early life stress (ELS) and youth-onset depression, a study in JAACAP, published by Elsevier, reports that individuals exposed to ELS were more likely to develop a major depressive disorder (MDD) in childhood or adolescence than individuals who had not been exposed to ELS.

Red light for stress
Researchers from the Institute of Industrial Science at The University of Tokyo have created a biphasic luminescent material that changes color when exposed to mechanical stress.

How do our cells respond to stress?
Molecular biologists reverse-engineer a complex cellular structure that is associated with neurodegenerative diseases such as ALS

How stress remodels the brain
Stress restructures the brain by halting the production of crucial ion channel proteins, according to research in mice recently published in JNeurosci.

Why stress doesn't always cause depression
Rats susceptible to anhedonia, a core symptom of depression, possess more serotonin neurons after being exposed to chronic stress, but the effect can be reversed through amygdala activation, according to new research in JNeurosci.

How plants handle stress
Plants get stressed too. Drought or too much salt disrupt their physiology.

Stress in the powerhouse of the cell
University of Freiburg researchers discover a new principle -- how cells protect themselves from mitochondrial defects.

Measuring stress around cells
Tissues and organs in the human body are shaped through forces generated by cells, that push and pull, to ''sculpt'' biological structures.

Cellular stress at the movies
For the first time, biological imaging experts have used a custom fluorescence microscope and a novel antibody tagging tool to watch living cells undergoing stress.

Read More: Stress News and Stress Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.