Defending food crops: Whitefly experimentation to prevent contamination of agriculture

November 08, 2013

On November 8th, JoVE, the Journal of Visualized Experiments, will introduce a new technique to aid in the development of defenses against diseases threatening food crops worldwide. The method, published under the title Transmitting Plant Viruses Using Whiteflies, is applicable to such at-risk crops as tomatoes and common bean plants. The whitefly method provides a means of interfering with the plant-contamination process as well as the cultivation of plants that are altogether resistant to infection.

"For example, the described technique is used to develop tomatoes with resistance to tomato yellow leaf curl virus, which is a big problem in tomato production in the southern U.S. and in many parts of the world," said Jane Polston, the principle investigator at the University of Florida's Department of Plant Pathology. In the article accompanying their JoVE video, Polston and her colleagues write that numerous genera of whitefly-transmitted plant viruses (such as Begomovirus, Carlavirus, Crinivirus, Ipomovirus, Torradovirus) are part of an emerging and economically significant group of pathogens affecting important food and fiber crops.

The technique includes reliably rearing whiteflies with a specific virus while omitting the possibility of cross-contamination to other viruses--an easily encountered problem because of the sheer number of whiteflies used in testing. Such contamination would jeopardize the results of an entire experiment. After exposing large numbers of a particular plant species to a specific whitefly-transmitted virus, a researcher can then note which individual plants resisted infection and why. This article outlines how to generate hundreds or thousands of infected plants year-round by exposing them to whiteflies each week. Therefore, the whitefly-assisted transmission method provides researchers with a powerful means for continued experimentation in developing plant defenses against the threat of whitefly-transmitted disease.

Polston said that she published this technique through JoVE's video format because it was difficult to explain it through traditional text-only journals. "I have never published like this before and wanted to try it," she said, "And it was very difficult to describe some of the details of this technique in writing. Video was a better approach."
-end-
About JoVE, the Journal of Visualized Experiments:

JoVE, the Journal of Visualized Experiments, is the first and only PubMed/MEDLINE-indexed, peer-reviewed journal devoted to publishing scientific research in a video format. Using an international network of videographers, JoVE films and edits videos of researchers performing new experimental techniques at top universities, allowing students and scientists to learn them much more quickly. JoVE has published video-protocols from an international community of nearly 8,000 authors in the fields of biology, medicine, chemistry, and physics.

The Journal of Visualized Experiments

Related Viruses Articles from Brightsurf:

Sorting out viruses with machine learning
Researchers at Osaka University created a machine-learning system to identify single viral particles that cause respiratory diseases, including coronavirus, using silicon nanopores.

The rafts used by viruses
The study may suggest new strategies to limit virus attacks and prevent or combat diseases like Sars and Covid-19 based on biomedical and engineering principles.

Animals keep viruses in the sea in balance
A variety of sea animals can take up virus particles while filtering seawater for oxygen and food.

Hundreds of novel viruses discovered in insects
New viruses which cause diseases often come from animals. Well-known examples of this are the Zika virus transmitted by mosquitoes, bird flu viruses, as well as the MERS virus which is associated with camels.

First video of viruses assembling
For the first time, researchers have captured images of the formation of individual viruses, offering a real-time view into the kinetics of viral assembly.

Plant viruses may be reshaping our world
A new review article appearing in the journal Nature Reviews Microbiology highlights the evolution and ecology of plant viruses.

Checkmate for hepatitis B viruses in the liver
Researchers at Helmholtz Zentrum M√ľnchen and the Technical University of Munich, working in collaboration with researchers at the University Medical Center Hamburg-Eppendorf and the University Hospital Heidelberg, have for the first time succeeded in conquering a chronic infection with the hepatitis B virus in a mouse model.

How viruses outsmart their host cells
Viruses depend on host cells for replication, but how does a virus induce its host to transcribe its own genetic information alongside that of the virus, thus producing daughter viruses?

Mobile, instant diagnosis of viruses
In a first for plant virology, a team from CIRAD recently used nanopore technology to sequence the entire genomes of two yam RNA viruses.

How ancient viruses got cannabis high
THC and CBD, bioactive substances produced by cannabis and sought by medical patients and recreational users, sprung to life thanks to ancient colonization of the plant's genome by viruses, U of T researchers have found.

Read More: Viruses News and Viruses Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.