New clues about the origins of familial forms of Amyotrophic lateral sclerosis

December 02, 2019

A team led by Brazilian researcher Elis Eleutherio, professor at the Federal University of Rio de Janeiro, in partnership with Tiago Outeiro, at University of Goettingen, Germany, made important progress in understanding the conformation and accumulation of certain proteins involved in lateral amyotrophic sclerosis (ALS).

"We believe protein accumulation is an important hallmark of ALS, and we still do not understand why the protein misbehaves and aggregates during the disease", explains Prof. Elis Eleutherio.

Amyotrophic lateral sclerosis (ALS) is a progressive and devastating neurodegenerative disorder affecting 1 to 3 individuals in 100.000, and is more prevalent in people between 55-75 years of age. The disease affects, primarily, a population of neurons known as 'motor neurons'. Patients suffer from irreversible motor paralysis, and become incapable of speaking, swallowing, or breathing as the disease progress.

Most ALS cases are sporadic, with no defined genetic origin, and only the minority is familial, with known associated genetic alterations. Certain familial forms of ALS (fALS) are associated with genetic alterations in the gene encoding for a protein known as superoxide dismutase 1 (Sod1), that cause alterations in the folding and function of the protein.

The study, published in the journal Proceedings of the National Academy of Sciences (PNAS), allowed scientists to understand the interaction between normal and mutant protein, which causes alteration of protein accumulation in the cell, but also impairs the function of Sod1 protein, thus contributing to the development of the disease. For the group, this discovery opens new perspectives for the treatment of ALS.

Sod1 is a protein that plays a role, among others, in the protection against oxidative damage in our cells. In some ALS cases, altered Sod1 protein accumulates inside neuronal cells and, researchers believe, cause damage to the neurons, leading to their death. Importantly, normal Sod1 protein, present in sporadic cases of ALS, can also misfold and accumulate, suggesting this is a central problem in ALS.

In the study, the researchers used simple experimental models, such as bakery yeast, used to make beer, wine and bread, and human cells, in order to better understand the context of protein aggregation in the disease. They also used a strategy that mimics the genetic context of fALS, where most patients carry one copy of the normal Sod1 protein, and one copy carrying a genetic alteration. "In patients, we think that the presence of a mutant copy of Sod1 alters the behavior of the normal copy", explains Dr. Aline Brasil, the first author of the study.

"By taking advantage of novel genetic manipulation tools, and powerful molecular imaging approaches, that enable the direct visualization of protein complexes in the cell (a technique known as BiFC), we were able to detect 'hetero-complexes' formed by normal and abnormal (mutant) Sod1 protein", said Prof. Tiago Outeiro, leader of the German team that participated in the study.

The research opens novel perspectives for therapeutic intervention, that the authors hope to continue to explore in the near future, such as the specific removal of mutant Sod1 protein.

"In a time when the scientific and education system in Brazil suffers from uncertainty, it is important to demonstrate that we can be competitive and conduct research that will contribute to society and may, ultimately, help change the lives of those affected by such devastating diseases", Prof. Elis Eleutherio concludes.
Prof. Dr. Elis Eleutherio is Associate Professor of the Federal University of Rio de Janeiro (UFRJ), where she coordinates the Laboratory of Investigation of Stress Factors (LIFE - At LIFE, Prof. Eleutherio and her group are currently working on the cell response to stress conditions, especially oxidative stress, which has been linked to the aging process and age-related diseases, such as cancer and ALS.

Prof. Dr. Tiago F. Outeiro is the Director of the Department of Experimental Neurodegeneration at the University Medical Center Goettingen, Goettingen, Germany, and he is a Professor of Neurodegeneration at Newcastle University. He is currently a visiting Professor at UFRJ. Prof. Outeiro is an expert in the field of neurogenerative diseases, focusing on the study of the molecular mechanisms underlying Parkinson's, Alzheimer's and ALS.

The study was funded by the Coordination for the Improvement of Higher Education Personnel, Ministry of Education (CAPES / MEC), the Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro (FAPERJ), the National Council for Scientific and Technological Development (CNPq), and the German Academic Exchange Service (DAAD).

Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem (INBEB)

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