Nav: Home

Vaping robots measure e-cig vapor delivered to lung cells in the lab

December 08, 2016

New research proves that e-cigarette aerosol droplets are effectively delivered to cell surfaces in lab-based biological tests.

'There have been several studies comparing the impact of e-cigarette vapour with that of cigarette smoke on cellular models, and there are a lot of great data out there,' said Dr James Murphy, Head of Risk Substantiation at British American Tobacco.

'When there is a partial response or no response at all, this can be interpreted as e-cigarette vapour having a reduced biological impact compared to cigarette smoke. But what if it just means we are losing the aerosol and exposing the system to air,' he said. 'We had to be sure. These latest results suggest that we can be confident that we are effectively delivering e-cig aerosol to cells in biological tests and that we can be confident, therefore, in the results of our biological tests,'

The results are published today in Chemistry Central Journal.

The cellular tests mimic key events in the development of tobacco-related diseases like cardiovascular disease or chronic obstructive pulmonary disease. They form part of a weight of evidence approach to assess the reduced risk potential of e-cigarettes and other next generation products.

Two different smoking/vaping robots were used to produce aerosol from a reference cigarette (3R4F) and Vype ePen, a commercially available e-cigarette. The deposited particle mass in exposure chambers was measured, as well as the amount of deposited nicotine -- Exposure chambers are used to expose human lung cells to aerosol in the lab and to assess the impact of that aerosol (smoke or vapour) on the health of those cells.

The results show that, on a puff by puff basis and at a common dilution, the e-cigarette aerosol deposited greater mass than cigarette smoke in both systems. In contrast, nicotine delivery was much greater from the cigarette than from the e-cigarette.

'It may seem counter intuitive that the aerosol that delivered the most mass had the least impact, but it's about what that mass represents,' explains Murphy.

The aerosol particles produced by smoke and vapour may appear similar but they are compositionally very different. Smoke is drier and stickier and therefore lighter than the glycerine based e-cigarette droplets, which are moist and tend to be heavier.

In addition, smoke droplets carry the products of combustion: thousands of chemicals and hundreds of toxicants. Whereas e-cigarette aerosol droplets contain the aerosolised form of the 4 main ingredients that make up e-liquids: humectants, water, nicotine and flavouring.

'This means the mass deposited on the cells by the e-cigarette vapour is compositionally very different to that deposited by cigarette smoke,' says Murphy.

Many in the public health community believe e-cigarettes offer great potential for reducing the public health impact of smoking. Public Health England, an executive body of the UK Department of Health, recently published a report saying that the current expert estimate is that using e-cigarettes is around 95% safer than smoking cigarettes, although more research is needed. The Royal College of Physicians have said that the public can be reassured that e-cigarettes are much safer then smoking and that they should be widely promoted as an alternative to cigarettes.

R&D at British American Tobacco

Related Smoking Articles:

Telomere length unaffected by smoking
A new study has surprised the medical world, finding that smoking does not shorten the length of telomeres -- a marker at the end of our chromosomes that is widely accepted as an indicator of aging.
Want to quit smoking? Partner up
Kicking the habit works best in pairs. That's the main message of a study presented today at EuroPrevent 2019, a scientific congress of the European Society of Cardiology (ESC).
Smoking and mortality in Asia
In this analysis of data from 20 studies conducted in China, Japan, South Korea, Singapore, Taiwan and India with more than 1 million participants, deaths associated with smoking continued to increase among men in Asia grouped by the years in which they were born.
Predictors of successfully quitting smoking among smokers registered at the quit smoking clinic at a public hospital in northeastern Malaysia
In the current issue of Family Medicine and Community Health, Nur Izzati Mohammad et al. consider how cigarette smoking is one of the risk factors leading to noncommunicable diseases such as cardiovascular and respiratory system diseases and cancer.
Restaurant and bar smoking bans do reduce smoking, especially among the highly educated
Smoking risk drops significantly in college graduates when they live near areas that have completely banned smoking in bars and restaurants, according to a new study in the American Journal of Epidemiology.
How the UK smoking ban increased wellbeing
Married women with children reported the largest increase in well-being following the smoking bans in the UK in 2006 and 2007 but there was no comparable increase for married men with children.
Smoking study personalizes treatment
A simple blood test is allowing Vanderbilt University Medical Center (VUMC) researchers to determine which patients should be prescribed varenicline (Chantix) to stop smoking and which patients could do just as well, and avoid side effects, by using a nicotine patch.
A biophysical smoking gun
While much about Alzheimer's disease remains a mystery, scientists do know that part of the disease's progression involves a normal protein called tau, aggregating to form ropelike inclusions within brain cells that eventually strangle the neurons.
A case where smoking helped
A mutation in the hemoglobin of a young woman in Germany was found to cause her mild anemia.
No safe level of smoking
People who consistently smoked an average of less than one cigarette per day over their lifetime had a 64 percent higher risk of earlier death than people who never smoked.
More Smoking News and Smoking Current Events

Best Science Podcasts 2019

We have hand picked the best science podcasts for 2019. Sit back and enjoy new science podcasts updated daily from your favorite science news services and scientists.
Now Playing: TED Radio Hour

Rethinking Anger
Anger is universal and complex: it can be quiet, festering, justified, vengeful, and destructive. This hour, TED speakers explore the many sides of anger, why we need it, and who's allowed to feel it. Guests include psychologists Ryan Martin and Russell Kolts, writer Soraya Chemaly, former talk radio host Lisa Fritsch, and business professor Dan Moshavi.
Now Playing: Science for the People

#538 Nobels and Astrophysics
This week we start with this year's physics Nobel Prize awarded to Jim Peebles, Michel Mayor, and Didier Queloz and finish with a discussion of the Nobel Prizes as a way to award and highlight important science. Are they still relevant? When science breakthroughs are built on the backs of hundreds -- and sometimes thousands -- of people's hard work, how do you pick just three to highlight? Join host Rachelle Saunders and astrophysicist, author, and science communicator Ethan Siegel for their chat about astrophysics and Nobel Prizes.