Nav: Home

Patient prostate tissue used to create unique model of prostate cancer biology

December 15, 2016

WASHINGTON (Dec. 15, 2016) -- For the first time, researchers have been able to grow, in a lab, both normal and primary cancerous prostate cells from a patient, and then implant a million of the cancer cells into a mouse to track how the tumor progresses. The achievement, say researchers at Georgetown University Medical Center who led the research, represents a critical advance in the effort to understand the origin and drivers of this puzzling cancer -- the most common in men. The study was published online today in Oncotarget.

"This is a new and much-needed platform for prostate cancer research. By matching normal and cancer cells from a patient, we can now study the differences -- what molecules are key to tumor development and growth, and, ultimately, match treatments that might disable this cancer," says the study's senior investigator, associate professor of pathology, Xuefeng Liu, MD, a member of the Center for Cell Reprogramming (CCR) at Georgetown University Medical Center.

The breakthrough was possible because the research team used conditional reprogramming (CR), a laboratory technique, developed and described by Liu, Richard Schlegel, MD, PhD, director of the CCR, and their colleagues at Georgetown in 2011, that makes it possible to continuously grow cells in a laboratory indefinitely. The method uses special "feeder" cells and a chemical inhibitor.

"This is the only system that can grow healthy and cancer cells as if they were just extracted from a patient, and expand them -- a million new cells can be grown in a week -- as long as needed," he says.

The CR method is being developed for a number of uses, such as living biobanks, personalized and regenerative medicine, and this study, using donated tissue from a 57 year-old man who underwent a radical prostatectomy, demonstrates the first steps needed towards those goals in prostate cancer. Previous studies have proven the utility of CR in a variety of tissue types, including breast, lung, and colon cancer. Liu says many labs around the world are now using this technique, which is called "conditional reprogramming."

"Prostate cancer is highly heterogenetic -- it is different person to person, can be slow growing or rapidly aggressive, or both over time. We really don't understand the basic biology of prostate cancer and that makes it very difficult to find targeted therapies," Liu says. "The use of matched patient-derived cells provides a unique model for studies of early prostate cancer."

In this proof-of-principle study, the researchers showed, using DNA sequencing and karyotyping technologies, that the patient's unique cell characteristics were maintained in both normal and tumor CR laboratory cells. This means nothing genetically changed due to the CR laboratory technique, the researchers say. Investigators also demonstrated the malignant properties of tumor cells compared to the matched normal cells. These are all hallmarks of tumor development, Liu says.

"Now we can compare what is different between the patient's normal and cancerous cells, and what changes when the cancer cells are allowed to morph into an advancing tumor," he says. "We will then use this technique to explore prostate tissues from other cancer patients. Comparisons between what happens within an individual patient's tissue, and then between patients, will give us priceless information about how we can best diagnose this baffling disease and treat it appropriately."

Study co-authors include Olga A. Timofeeva, PhD, Nancy Palechor-Ceron, DMD, Hang Yuan, PhD, Ewa Krawczyk, PhD, Geeta Upadhyay, PhD, Aleksandra Dakic, PhD, Songtao Yu, MD, Shuang Fang, MD, Sujata Choudhury, PhD, Xueping Zhang, PhD, Yun-Ling Zheng, MD, PhD, Chris Albanese, PhD, Richard Schlegel, MD, PhD, Xiaogang Zhong, PhD, Andrew Ju, MD, and Anatoly Dritschilo, MD, from Georgetown University Medical Center; Guanglei Li, Geng Liu, and Yong Hou from Beijing Genome Research Institute, Shenzhen, Guangdong, China; Myeong-Seon Lee from Cheongju University, the Republic of Korea; Han C Dan, from the University of Maryland; and Youngmi Ji and Johng Rhim, MD, from the Uniformed Services University of the Health Sciences, Bethesda, Maryland.

Georgetown University has pending patent applications in US and internationally for conditional cell reprogramming and has been awarded a US patent by the United States Patent Office (9,279,106). This technology has been licensed exclusively to a company for further development and commercialization. Georgetown University and the inventors (Liu and Schlegel) receive payments and potential royalties from Propagenix. Schlegel is also a co-founder in the company that has a license to this technology.
-end-
This work was partially supported by grants from the National Institutes of Health (R33CA177466, R21CA180524, P30 CA051008, TL1-TR001431), the Department of Defense (PC140268 and W81XWH-13-1-0327), and with internal grant support from Center for Cell Reprogramming at GUMC.

About Georgetown Lombardi Comprehensive Cancer Center

Georgetown Lombardi Comprehensive Cancer Center is designated by the National Cancer Institute as a comprehensive cancer center -- the only cancer center of its kind in the Washington, DC area. A part of Georgetown University Medical Center and MedStar Georgetown University Hospital, Georgetown Lombardi seeks to improve the diagnosis, treatment, and prevention of cancer through innovative basic and clinical research, patient care, community education and outreach, and the training of cancer specialists of the future. Connect with Georgetown Lombardi on Facebook (Facebook.com/GeorgetownLombardi) and Twitter (@LombardiCancer).

About Georgetown University Medical Center

Georgetown University Medical Center (GUMC) is an internationally recognized academic medical center with a three-part mission of research, teaching and patient care (through MedStar Health). GUMC's mission is carried out with a strong emphasis on public service and a dedication to the Catholic, Jesuit principle of cura personalis -- or "care of the whole person." The Medical Center includes the School of Medicine and the School of Nursing & Health Studies, both nationally ranked; Georgetown Lombardi Comprehensive Cancer Center, designated as a comprehensive cancer center by the National Cancer Institute; and the Biomedical Graduate Research Organization, which accounts for the majority of externally funded research at GUMC including a Clinical and Translational Science Award from the National Institutes of Health. Connect with GUMC on Facebook (Facebook.com/GUMCUpdate), Twitter (@gumedcenter) and Instagram (@gumedcenter).

Georgetown University Medical Center

Related Prostate Cancer Articles:

First prostate cancer therapy to target genes delays cancer progression
For the first time, prostate cancer has been treated based on the genetic makeup of the cancer, resulting in delayed disease progression, delayed time to pain progression, and potentially extending lives in patients with advanced, metastatic prostate cancer, reports a large phase 3 trial.
Men taking medications for enlarged prostate face delays in prostate cancer diagnosis
University of California San Diego School of Medicine researchers report that men treated with medications for benign prostatic hyperplasia (enlarged prostate) experienced a two-year delay in diagnosis of their prostate cancer and were twice as likely to have advanced disease upon diagnosis.
CNIO researchers confirm links between aggressive prostate cancer and hereditary breast cancer
The study has potential implications for families with members suffering from these types of tumours who are at an increased risk of developing cancer.
Distinguishing fatal prostate cancer from 'manageable' cancer now possible
Scientists at the University of York have found a way of distinguishing between fatal prostate cancer and manageable cancer, which could reduce unnecessary surgeries and radiotherapy.
Researchers find prostate cancer drug byproduct can fuel cancer cells
A genetic anomaly in certain men with prostate cancer may impact their response to common drugs used to treat the disease, according to new research at Cleveland Clinic.
ASCO and Cancer Care Ontario update guideline on radiation therapy for prostate cancer
The American Society of Clinical Oncology (ASCO) and Cancer Care Ontario today issued a joint clinical practice guideline update on brachytherapy (internal radiation) for patients with prostate cancer.
Patient prostate tissue used to create unique model of prostate cancer biology
For the first time, researchers have been able to grow, in a lab, both normal and primary cancerous prostate cells from a patient, and then implant a million of the cancer cells into a mouse to track how the tumor progresses.
Moffitt Cancer Center awarded $3.2 million grant to study bone metastasis in prostate cancer
Moffitt researchers David Basanta, Ph.D., and Conor Lynch, Ph.D., have been awarded a U01 grant to investigate prostate cancer metastasis.
Prostate cancer discovery may make it easier to kill cancer cells
A newly discovered connection between two common prostate cancer treatments may soon make prostate cancer cells easier to destroy.
New test for prostate cancer significantly improves prostate cancer screening
A study from Karolinska Institutet in Sweden shows that a new test for prostate cancer is better at detecting aggressive cancer than PSA.
More Prostate Cancer News and Prostate Cancer Current Events

Top Science Podcasts

We have hand picked the top science podcasts of 2019.
Now Playing: TED Radio Hour

Risk
Why do we revere risk-takers, even when their actions terrify us? Why are some better at taking risks than others? This hour, TED speakers explore the alluring, dangerous, and calculated sides of risk. Guests include professional rock climber Alex Honnold, economist Mariana Mazzucato, psychology researcher Kashfia Rahman, structural engineer and bridge designer Ian Firth, and risk intelligence expert Dylan Evans.
Now Playing: Science for the People

#541 Wayfinding
These days when we want to know where we are or how to get where we want to go, most of us will pull out a smart phone with a built-in GPS and map app. Some of us old timers might still use an old school paper map from time to time. But we didn't always used to lean so heavily on maps and technology, and in some remote places of the world some people still navigate and wayfind their way without the aid of these tools... and in some cases do better without them. This week, host Rachelle Saunders...
Now Playing: Radiolab

Dolly Parton's America: Neon Moss
Today on Radiolab, we're bringing you the fourth episode of Jad's special series, Dolly Parton's America. In this episode, Jad goes back up the mountain to visit Dolly's actual Tennessee mountain home, where she tells stories about her first trips out of the holler. Back on the mountaintop, standing under the rain by the Little Pigeon River, the trip triggers memories of Jad's first visit to his father's childhood home, and opens the gateway to dizzying stories of music and migration. Support Radiolab today at Radiolab.org/donate.