Nanometer-scale growth of cone cells tracked in living human eye

December 20, 2011

WASHINGTON, Dec. 20 -- Humans see color thanks to cone cells, specialized light-sensing neurons located in the retina along the inner surface of the eyeball. The actual light-sensing section of these cells is called the outer segment, which is made up of a series of stacked discs, each about 30 nanometers (billionths of a meter) thick. This appendage goes through daily changes in length. Scientists believe that a better understanding of how and why the outer segment grows and shrinks will help medical researchers identify potential retinal problems. But the methods usually used to image the living human eye are not sensitive enough to measure these miniscule changes. Now, vision scientists at Indiana University in Bloomington have come up with a novel way to make the measurements in a living human retina by using information hidden within a commonly used technique called optical coherence tomography (OCT). They discuss their results in the Optical Society's (OSA ( open-access journal Biomedical Optics Express(

To make an OCT scan of the retina, a beam of light is split into two. One beam scatters off the retina while the other is preserved as a reference. The light waves begin in synch, or in phase, with each other; when the beams are reunited, they are out of phase, due to the scattering beam's interactions with retinal cells. Scientists can use this phase information to procure a precise measurement of a sample's position. But since in this case their samples were attached to live subjects, the researchers had to adapt these typical phase techniques to counteract any movements that the subjects' eyes might insert into the data.

Instead of measuring the phase of a single interference pattern, the researchers measured phase differences between patterns originating from two reference points within the retinal cells: the top and bottom of the outer segment. The team used this hidden phase information to measure microscopic changes in hundreds of cones, over a matter of hours, in two test subjects with normal vision. Researchers found they could resolve the changes in length down to about 45 nanometers, which is just slightly longer than the thickness of a single one of the stacked discs that make up the outer segment. The work shows that the outer segments of the cone cells grow at a rate of about 150 nanometers per hour, which is about 30 times faster than the growth rate of a human hair.
Paper: "Phase-sensitive imaging of the outer retina using optical coherence tomography and adaptive optics (," Biomedical Optics Review, Vol. 3, Issue 1, pp. 104-124 (2012).

EDITOR'S NOTE: This summary is part of OSA's monthly Biomedical Optics Express tip sheet. To subscribe, email or follow @OpticalSociety on Twitter. For images or interviews with authors, please contact Angela Stark, or 202-416-1443.

About Biomedical Optics Express

Biomedical Optics Express is OSA's principal outlet for serving the biomedical optics community with rapid, open-access, peer-reviewed papers related to optics, photonics and imaging in the life sciences. The journal scope encompasses theoretical modeling and simulations, technology development, and biomedical studies and clinical applications. It is published by the Optical Society and edited by Joseph A. Izatt of Duke University. Biomedical Optics Express is an open-access journal and is available at no cost to readers online at

About OSA

Uniting more than 130,000 professionals from 175 countries, the Optical Society (OSA) brings together the global optics community through its programs and initiatives. Since 1916 OSA has worked to advance the common interests of the field, providing educational resources to the scientists, engineers and business leaders who work in the field by promoting the science of light and the advanced technologies made possible by optics and photonics. OSA publications, events, technical groups and programs foster optics knowledge and scientific collaboration among all those with an interest in optics and photonics. For more information, visit

The Optical Society

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