Study confirms Ebola and Marburg virus DNA vaccines are safe and immunogenic in Africa

December 23, 2014

Dec. 23, 2014 (SILVER SPRING, Md.) - Results from the first Ebola vaccine clinical trial conducted in Africa (in 2009-2010) reveal a vaccine candidate produces the same immune response seen in the United States in an African setting. Importantly, this vaccine candidate is a precursor to candidates currently under evaluation for potential testing in West Africa. The findings of this 2009-2010 study, published online today in The Lancet, describe the successful execution and analysis of a Phase 1 clinical trial of two DNA vaccine candidates, one for the Ebola virus and the other for the closely related Marburg virus. The trial, conducted in Uganda, also demonstrated that the vaccines for the Ebola and Marburg viruses could be combined safely.

"The RV247 study represents the first Ebola vaccine trial conducted in Africa. Since immune responses to vaccines can differ around the world, these findings are encouraging for the development of an effective Ebola vaccine for Africa," said Merlin Robb, M.D., Director for Clinical Research at the US Military HIV Research Program (MHRP), whose site in Uganda, Makerere University Walter Reed Project (MUWRP), conducted the study. "Given separately and concurrently, both vaccines were safe, well-tolerated, and elicited antigen-specific humoral and cellular immune responses."

Outbreaks of Ebola and Marburg virus, both filoviruses, have occurred sporadically since their discovery in 1976 and 1967, respectively. The 2014 Ebola outbreak concentrated in three West African nations, is the largest on record and notable for extension into densely populated cities. Finding comparable safety and immunogenicity in U.S. and Ugandan populations provided proof of principle that a protective, multivalent filovirus vaccine is attainable.

The products used in the trial were tested in the U.S. prior to the launch of the study in Uganda and were developed by the Vaccine Research Center (VRC) at the National Institute of Allergy and Infectious Diseases (NIAID). These findings have led to the clinical evaluation of a more potent vaccine developed by the VRC, a Chimpanzee Adenovirus type 3 vector (cAd3) encoding the same wild-type glycoprotein antigen. This vaccine entered clinical trials in September 2014. MUWRP will begin testing this vaccine in early January 2015. As part of this upcoming study, some Ugandan volunteers from the 2009 DNA study will receive a boost, or additional injection, with the new vaccine candidate to explore a more long lasting effect of vaccination.

MUWRP was selected by the NIAID to conduct RV 247 based on their expertise in conducting HIV vaccine trials. Following a deadly 2007 outbreak of a new strain of Ebola virus in the Bundibugyo region of Uganda, MUWRP developed a strong interest in creating a vaccine that cut across subtypes of the virus. A total of 373 volunteers were screened for eligibility, and 108 volunteers were enrolled into the study between November 2009 and April 2010.

"The Ugandan scientific community and general population were very interested in participating in vaccine research relevant to Ugandan public health, including filovirus vaccine research," said Hannah Kibuuka, M.D., the principal investigator of the RV 247 Ebola study in Uganda. "Many of the people who volunteered were aware of the impact of Ebola outbreaks in Uganda. Most were educated at the university level."
MUWRP is part of the US Military HIV Research Program, which has an extensive medical research infrastructure to conduct clinical studies in endemic areas. When it conducted this first Ebola vaccine study, MUWRP already had clinical research experience with vaccines and conducted them in accordance with Good Clinical Practices. They also had established capabilities in data management and community engagement, and laboratory capabilities to measure safety parameters and collect and manage samples so that immune responses could be evaluated.

MHRP, centered at the Walter Reed Army Institute of Research, has other sites in Africa that have also expressed interest in participating in future Ebola vaccine studies. Plans are underway at the MHRP site in Nigeria to participate in a large phase 2 study of the cAd3 Ebola vaccine. "The US Military has partnered with the African scientific and general community for more than 40 years," according to MHRP director Col. Nelson Michael, M.D., Ph.D. "We have worked on the ground for decades with host nations to build sustainable infrastructure to support infectious diseases research in areas such as HIV, malaria, dengue virus and Ebola. We work closely with NIAID scientists to identify and address key research areas that will help speed progress to develop globally effective vaccines for global public health threats."

See NIAID statement on this study. The identifier for this study is NTC00997607

Citation: H Kibuuka et al. Safety and immunogenicity of Ebola virus and Marburg virus glycoprotein DNA vaccines assessed separately and concomitantly healthy Ugandan adults: a phase 1B randomised, double-blind, placebo-controlled clinical trial. The Lancet DOI: 10.1016/S0140-6736(14)62385-0 (2014).

About MHRP

The US Military HIV Research Program (MHRP) at the Walter Reed Army Institute of Research conducts research to develop an effective HIV vaccine and integrates prevention, treatment, diagnosis and monitoring as part of a global effort to protect troops and reduce the impact of HIV worldwide. MHRP has developed six state-of-the-art international research sites in the Africa and Asia. In 2009, MHRP announced results of an Army-sponsored clinical trial in Thailand that demonstrated for the first time a modest ability to protect against HIV infection, reducing the number of infections by 31.2 percent. The program successfully collaborates on HIV prevention care and treatment services, funded by the President's Emergency Plan for AIDS Relief (PEPFAR), with African militaries and in the communities where it conducts research. For more information, visit or find MHRP on Facebook,, and Twitter at @MHRPInfo.

An interview with an RV247 Ebola vaccine trial volunteer is on the MHRP website.

About the Walter Reed Army Institute of Research (WRAIR)

WRAIR is a leader in global efforts against the world's most pervasive and high impact infectious diseases, such as malaria, HIV/AIDS, Ebola, and dengue. Infectious diseases pose a significant and persistent threat to force protection and readiness and while the primary mission of Army medical research is protection of the U.S. Service and their family members, vaccines and treatments developed by Army researchers also benefit global public health.

Around the world, WRAIR infectious disease physicians and scientists work alongside civilian researchers and medical professionals to test and develop products that will ultimately reduce the impact of some of the world's most lethal diseases.

Some of the WRAIR's enduring contributions to global health include: For more information on WRAIR, visit: http://wrair-

The U.S. Military HIV Research Program (MHRP)

Related Immune Response Articles from Brightsurf:

Boosting chickens' own immune response could curb disease
Broiler chicken producers the world over are all too familiar with coccidiosis, a parasite-borne intestinal disease that stalls growth and winnows flocks.

Cells sacrifice themselves to boost immune response to viruses
Whether flu or coronavirus, it can take several days for the body to ramp up an effective response to a viral infection.

Children's immune response more effective against COVID-19
Children and adults exhibit distinct immune system responses to infection by the virus that causes COVID-19, a finding that helps explain why COVID-19 outcomes tend to be much worse in adults, researchers from Yale and Albert Einstein College of Medicine report Sept.

Which immune response could cause a vaccine against COVID-19?
Immune reactions caused by vaccination can help protect the organism, or sometimes may aggravate the condition.

Obesity may alter immune system response to COVID-19
Obesity may cause a hyperactive immune system response to COVID-19 infection that makes it difficult to fight off the virus, according to a new manuscript published in the Endocrine Society's journal, Endocrinology.

Immune response to Sars-Cov-2 following organ transplantation
Even patients with suppressed immune systems can achieve a strong immune response to Sars-Cov-2.

'Relaxed' T cells critical to immune response
Rice University researchers model the role of relaxation time as T cells bind to invaders or imposters, and how their ability to differentiate between the two triggers the body's immune system.

A novel mechanism that triggers a cellular immune response
Researchers at Baylor College of Medicine present comprehensive evidence that supports a novel trigger for a cell-mediated response and propose a mechanism for its action.

Platelets exacerbate immune response
Platelets not only play a key role in blood clotting, but can also significantly intensify inflammatory processes.

How to boost immune response to vaccines in older people
Identifying interventions that improve vaccine efficacy in older persons is vital to deliver healthy ageing for an ageing population.

Read More: Immune Response News and Immune Response Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to