New roles for protein modifications in cell migration

December 25, 2003

In every stage of development, cells are directed to make their way through the body to contribute to the building of or take up residence in new tissues and organs. The guidance of cell migration is achieved by signals intrinsic to the cell or from its surrounding environment. In work published on December 21 in the online edition of Nature Cell Biology, the RIKEN Center for Developmental Biology (CDB) Laboratory for Cell Migration, under team leader Kiyoji Nishiwaki, has demonstrated the role of a protein modification known as glycosylation in guiding the migration of gonadal cells in the nematode, C. elegans.

This study, conducted in collaboration with researchers from the Japanese National Institute of Advanced Industrial Science and Technology, and the Universities of Nagoya (Japan) and Toronto (Canada), is the first to reveal the function of glycosylation in the action of an ADAM-family protease, a finding of potential significance to the study and treatment of human autoimmune diseases and other disorders that involve defects in ADAM-family protein function.

Interactions between the basement membrane of the migrating gonad and that of the body wall are one of the keystone processes of gonadal development. The C. elegans gonad is U-shaped, the result of the directed migration of cells known as distal tip cells present at the leading ends of the developing gonad in larvae. The gene mig-17, which Nishiwaki previously showed to be essential to this guided movement, is expressed in muscle cells and secreted into the body cavity where it binds to the migrating gonadal tip cells. Distal tip cells in worms engineered to lack the MIG -17 protein fail to steer cell migration properly, resulting in abnormal development and a emeanderingf gonad phenotype. However, the means by which MIG-17 coordinates the timing and direction of the developing gonadfs progress though the larval body has remained unknown.

MIG-17 is an ADAM (a disintegrin and metalloprotease) protease, a family of secreted or membrane proteins first identified as components of various snake venoms, where they have hemorrhagic and anti-coagulatory effects. ADAM-family proteases have been implicated in a number of human physiological and pathological processes, including blood coagulation disorders, rheumatoid arthritis and asthma. These proteins act to proteolytically release membrane-bound growth factors or to disrupt components of extracellular matrices such as collagen and proteoglycans (which help to build skin and soft tissue), and their roles in development and differentiation are the focus of interest in many research labs.

Nishiwakifs team analyzed a number of mutations that result in aberrant gonadal phenotypes similar to those seen in mig-17 mutants, including the mutation of the gene that encodes MIG-23. This protein was found to be structurally related to nucleoside diphosphatases (NDPases), glycosylating proteins found in the Golgi apparatus, the intracellular processing center where proteins are modified, sorted and released from the cell. Glycosylation involves the addition of a sugar to a site on a protein chain, a post-translational modification whose roles are incompletely understood, but which is thought to serve a regulatory function in some signaling molecules. On further investigation, MIG-23 mutants showed reductions in NDPase activity. The wild-type MIG-23 protein successfully rescued yeast mutants for NDPase, lending solid support to the case for the NDPase nature of MIG-23.

The similarity between the MIG-23 and MIG-17 phenotypes suggested that the mutation of MIG-23 might result in abnormal MIG-17 glycosylation. Using gel electrophoresis, Nishiwaki and colleagues demonstrated that MIG-17 molecules in MIG-23 mutants had lower than normal molecular weights consistent with the loss of glycosylation, while immunoblotting studies confirmed that in these mutants MIG-17 failed to bind sugars at the normal levels. MIG-17 molecules designed with deficient glycosylation sites also failed to localize at the gonad and showed no ability to direct its migration. Genetic analyses revealed that MIG-23 is expressed in muscle cells, which reconciles well with MIG-17fs muscle cell origins. Taken together, these results indicate that MIG-17 molecules are secreted from muscle cells after glycosylation by MIG-23, a modification which enables their recruitment to the migrating gonad.

Although Golgi NDPases such as MIG-23 can conceivably modify a number of other secreted proteins, the Nishiwaki study indicates that the effects of the NDPase dysfunction are especially prominent in the abnormal glycosylation and function of an ADAM family protease, suggesting that glycosylation may play a more important role in the function of proteins in this family than it does in that of other secreted proteins. ADAM family proteases are known to be involved in a number of human diseases, and the question of whether glycosylation defects play a role in their pathogenesis merits further investigation. Research such as this provides insights into basic biological mechanisms that may one day make it possible to treat a range of health conditions by restoring normal ADAM protease glycosylation in cases in which it is aberrant.

RIKEN Center for Developmental Biology

Related Proteins Articles from Brightsurf:

New understanding of how proteins operate
A ground-breaking discovery by Centenary Institute scientists has provided new understanding as to the nature of proteins and how they exist and operate in the human body.

Finding a handle to bag the right proteins
A method that lights up tags attached to selected proteins can help to purify the proteins from a mixed protein pool.

Designing vaccines from artificial proteins
EPFL scientists have developed a new computational approach to create artificial proteins, which showed promising results in vivo as functional vaccines.

New method to monitor Alzheimer's proteins
IBS-CINAP research team has reported a new method to identify the aggregation state of amyloid beta (Aβ) proteins in solution.

Composing new proteins with artificial intelligence
Scientists have long studied how to improve proteins or design new ones.

Hero proteins are here to save other proteins
Researchers at the University of Tokyo have discovered a new group of proteins, remarkable for their unusual shape and abilities to protect against protein clumps associated with neurodegenerative diseases in lab experiments.

Designer proteins
David Baker, Professor of Biochemistry at the University of Washington to speak at the AAAS 2020 session, 'Synthetic Biology: Digital Design of Living Systems.' Prof.

Gone fishin' -- for proteins
Casting lines into human cells to snag proteins, a team of Montreal researchers has solved a 20-year-old mystery of cell biology.

Coupled proteins
Researchers from Heidelberg University and Sendai University in Japan used new biotechnological methods to study how human cells react to and further process external signals.

Understanding the power of honey through its proteins
Honey is a culinary staple that can be found in kitchens around the world.

Read More: Proteins News and Proteins Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to