Microfluidic device rapidly orients hundreds of embryos for high-throughput experiments

December 26, 2010

Researchers have developed a microfluidic device that automatically orients hundreds of fruit fly and other embryos to prepare them for research. The device could facilitate the study of such issues as how organisms develop their complex structures from single cells -- one of the most fascinating aspects of biology.

Scientists know that among an embryo's first major developments is the establishment of its dorsoventral axis, which runs from its back to its belly. Determining how this axis development unfolds -- specifically the presence and location of proteins during the process -- requires the ability to simultaneously monitor large numbers of embryos with different genetic backgrounds at several time points.

"Collecting and analyzing the signaling and transcriptional patterns of the dorsoventral axis typically requires manual manipulation of individual embryos to stand them on their ends, making it difficult to conduct high-throughput experiments that can achieve statistically significant results," said Hang Lu, an associate professor in the Georgia Tech School of Chemical & Biomolecular Engineering.

To enable large-scale quantitative analyses of protein positional information along the dorsoventral axis, Lu designed a microfluidic device that reliably and robustly orients several hundred embryos in just a few minutes.

Details of the device design and results from proof-of-concept experiments with fruit fly embryos were published in the Dec. 26 advance online edition of the journal Nature Methods. This project was supported by the National Science Foundation, the National Institutes of Health, the Alfred P. Sloan Foundation and the DuPont Young Professor program.

Lu designed and fabricated the device with the help of Kwanghun Chung and Emily Gong, who worked on the project as Georgia Tech graduate and undergraduate students, respectively. Fabricated from polydimethylsiloxane (PDMS), the compact device is the size of a microscope slide and contains approximately 700 traps for embryos, which are shaped like grains of rice but smaller in size.

In operation, fluid flows through an "S"-shaped channel wide enough for embryos of any orientation to move easily through it. The fluid efficiently directs the embryos toward the traps, while sweeping out extra and improperly trapped embryos.

"The flow pattern significantly increased the frequency at which embryos contacted the traps and were loaded into them," explained Lu. "Experimentally, we found on average 90 percent of the embryos became trapped in the device, which will be valuable for studies that only have a small number of embryos available."

When an embryo approaches an empty trap, it experiences non‐uniform pressure and shear from the surrounding fluid. The resulting force flips the embryo vertically and inserts it into the cylindrical trap in an upright position, with its dorsoventral axis parallel to the ground. The embryo is then secured inside the trap, without any need for user intervention or control. The lock‐in feature allows the device to be disconnected from the rest of the hardware and transported for imaging or storage with the embryos enclosed.

"At one point, we mailed a microfluidic embryo trap array device full of trapped fruit fly embryos to our collaborators at Princeton University, and upon arrival, the embryos were still upright in their locked traps," said Lu.

To demonstrate the device's capabilities, Lu collaborated with Stanislav Shvartsman, an associate professor in the Department of Chemical and Biological Engineering at Princeton University, and his graduate student Yoosik Kim. The Princeton researchers used the device to quantify gradients of signaling molecules called morphogens in fixed embryos and also used it to monitor nuclear divisions in live embryos.

In one experiment, the Princeton researchers determined the spatial extent of the distribution of Dorsal, a transcription factor that initiates the dorsal‐to‐ventral patterning of the Drosophila embryo. They also demonstrated that this gradient could be quantitatively compared between wild-type and mutant embryos.

"The trap array device provided a significant increase in the number of fixed and live embryos we could image simultaneously and allowed us to accurately resolve issues of interest to developmental biologists today," explained Lu.

In the future, scientists should be able to adapt the microfluidic device for studies of pattern formation and morphogenesis in other model organisms, such as zebrafish or worm embryos. Results of those studies will be important to the scientific community because many genes controlling development are similar in worms, fruit flies and mammals.
-end-
This project was supported by the National Science Foundation (NSF) (Award No. DBI‐0649833) and the National Institutes of Health (NIH) (Award No. R21NS058465). The content is solely the responsibility of the principal investigator and does not necessarily represent the official views of the NSF or NIH.

Georgia Institute of Technology

Related Fruit Fly Articles from Brightsurf:

What can you learn by peering into a fruit fly's gut? It turns out a lot!
They say a picture is worth 1,000 words. But what about a real-time window into the complexity of the gastrointestinal system?

Study gives the green light to the fruit fly's color preference
In a study published in the journal Nature on Wednesday, University of Miami researchers made two unexpected discoveries.

Maleness-on-the-Y: A novel male sex determiner in major fruit fly pests
Becoming a male Mediterranean fruit fly relies on the newly identified Y-chromosome linked gene -- Maleness-on-the-Y (MoY) -- which encodes the small protein required to signal male sex determination during development, a new study shows.

Why so fly: MU scientists discover some fruit flies learn better than others
Fruit flies could one day provide new avenues to discover additional genes that contribute to a person's ability to learn and remember.

Fruit fly wing research reshapes understanding of how organs form
How do fruit flies grow their wings? Rutgers scientists discovered a surprising answer that could one day help diagnose and treat human genetic diseases.

Fruit fly promiscuity alters the evolutionary forces on males
Researchers in the Department of Zoology at Oxford University have demonstrated for the first time what effect female fruit flies having multiple partners has on sexual selection -- before and after mating.

Fruit fly protein could be new tool in tackling disease-carrying mosquitos
An insulin-binding protein in fruit flies could provide new opportunities for tackling disease-carrying mosquitos, such as malaria and yellow fever, scientists at the University of York have found.

Guiding flight: The fruit fly's celestial compass
Fruit flies use the sun to avoid flying in circles, according to new research.

When confronted, a single neuron helps a fruit fly change course
In the fruit fly, a single pair of brain neurons command backward locomotion in both larvae and adults, researchers report.

Fecal deposits reveal the fruit fly's pheromone flag
Fruit flies have a rich language of smell messages that they exchange, but now their secret is out.

Read More: Fruit Fly News and Fruit Fly Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.