Speeding up Ebola drug production

December 30, 2014

Researchers at the University of California, Davis will explore ways to speed production of the Ebola drug with a $200,000 rapid-response grant from the National Science Foundation.

Developed by Mapp Biopharmaceutical Inc. of San Diego, in collaboration with the U.S. government and partners in Canada, Zmapp is a cocktail of antibodies produced in and extracted from whole tobacco plants. The UC Davis team, including plant scientists, molecular biologists and chemical engineers, will attempt to produce the antibodies from plant cells grown in bioreactors instead of in whole plants.

Extracting the drug from whole plants is a proven process but production capacity is limited at this time, said Karen McDonald, professor of chemical engineering and materials science.

"Whereas if we can produce it in a bioreactor, a lot of biotech companies and contract manufacturers can do that, and it would allow for much more rapid production," McDonald said.

Mapp's technology uses a type of bacteria to transiently transfer the antibodies' DNA for the monoclonal antibodies into plants. The plants do not permanently carry the new DNA or pass it on to the next generation. A week or so after the transfer, the plants are ground up to extract the monoclonal antibodies.

The UC Davis team will use the same type of bacteria to infect plant cells, then attempt to grow them in the laboratory, starting with volumes of a few liters and scaling up to a 100-liter bioreactor. Biotech companies use similar methods to produce drugs and vaccines from cultures of animal cells, bacteria and yeast.

"This is about proof of concept," said Somen Nandi, managing director of the Global HealthShare Initiative at UC Davis, which aims to speed development of low-cost health-care TWO WORDS solutions for developing countries, including a rabies vaccine grown in tobacco plants. If successful, the technique could potentially be applied to other antibodies used as drugs or vaccines.
Other team members are Ray Rodriguez, UC Davis professor of molecular cell biology and director of the Global HealthShare Initiative; Abhaya Dandekar, UC Davis professor of plant sciences; and Professor Kazuhito Fujiyama, Osaka University, Japan.

University of California - Davis

Related Bacteria Articles from Brightsurf:

Siblings can also differ from one another in bacteria
A research team from the University of Tübingen and the German Center for Infection Research (DZIF) is investigating how pathogens influence the immune response of their host with genetic variation.

How bacteria fertilize soya
Soya and clover have their very own fertiliser factories in their roots, where bacteria manufacture ammonium, which is crucial for plant growth.

Bacteria might help other bacteria to tolerate antibiotics better
A new paper by the Dynamical Systems Biology lab at UPF shows that the response by bacteria to antibiotics may depend on other species of bacteria they live with, in such a way that some bacteria may make others more tolerant to antibiotics.

Two-faced bacteria
The gut microbiome, which is a collection of numerous beneficial bacteria species, is key to our overall well-being and good health.

Microcensus in bacteria
Bacillus subtilis can determine proportions of different groups within a mixed population.

Right beneath the skin we all have the same bacteria
In the dermis skin layer, the same bacteria are found across age and gender.

Bacteria must be 'stressed out' to divide
Bacterial cell division is controlled by both enzymatic activity and mechanical forces, which work together to control its timing and location, a new study from EPFL finds.

How bees live with bacteria
More than 90 percent of all bee species are not organized in colonies, but fight their way through life alone.

The bacteria building your baby
Australian researchers have laid to rest a longstanding controversy: is the womb sterile?

Hopping bacteria
Scientists have long known that key models of bacterial movement in real-world conditions are flawed.

Read More: Bacteria News and Bacteria Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.