Immunohistochemistry
Articles tagged with Immunohistochemistry
Rare laryngeal leiomyosarcoma successfully treated with surgery and adjuvant chemotherapy
A rare case of high-grade laryngeal leiomyosarcoma was successfully treated with extended laryngectomy, left neck dissection, and adjuvant chemotherapy. Complete surgical excision remains the cornerstone of therapy, while multidisciplinary-guided adjuvant treatment may benefit selected high-grade or high-risk patients.
Characteristics of CD4+T-cell reduction and pulmonary infections in critically ill immunocompromised patients
A retrospective study of 40 immunocompromised ICU patients found associations between low CD4+T-cell counts and specific lung pathogens. Patients with severe CD4 depletion had higher proportions of fungal infections, while moderate immunosuppression was linked to Streptococcus pneumoniae.
Rare pancreatic tumor case suggests distinctive calcification patterns in solid pseudopapillary neoplasms
A new case report describes a large, calcified solid pseudopapillary neoplasm of the pancreatic head, emphasizing the importance of multidisciplinary evaluation and long-term follow-up for monitoring recurrence. The case underscores the diagnostic value of integrating imaging, histopathology, and immunohistochemistry.
Deep learning-enabled virtual multiplexed immunostaining of label-free tissue for vascular invasion assessment
Researchers created a novel approach for simultaneous ERG, PanCK, and H&E image generation from label-free tissue sections, enhancing vascular invasion assessment accuracy and efficiency. The virtual multiplexed immunostaining method overcomes traditional IHC limitations, such as section-to-section variability and tissue loss.
Gut microbe–derived butyrate activates immune cells to enhance vaccine efficacy
A study published in Microbiome found that gut microbiome-derived butyrate activates immune cells to enhance vaccine efficacy by promoting T follicular helper (Tfh) cell activity and mucosal antibody production. This discovery highlights the crucial role of gut environment regulation in controlling infections and enhancing vaccine resp...
Tagging ‘fake targets’ for antigen-independent immunotherapy
Researchers developed a novel technology to attach 'fake targets' to tumor cells, enabling immune cells to attack regardless of antigen presence. The Univody platform showed promising results in animal models, suppressing tumor growth and triggering broader immune activation.
Epithelioid hemangioendothelioma: Rare vascular tumor mimics common hematoma
Epithelioid hemangioendothelioma is a rare and potentially malignant vascular tumor that can be mistaken for benign conditions. A new case report highlights the diagnostic challenges raised by rare tumors and emphasizes the need for vigilant evaluation of slow-growing swellings.
A natural compound against stroke? Psychoactive agent protects brain vessels and reduces inflammation
Researchers found that DMT reduces infarct volume and edema formation in rat stroke models by restoring blood-brain barrier function and reducing inflammatory cytokines. The compound's dual action may complement existing treatments, offering a novel approach to stroke therapy.
Mesonephric carcinoma and mesonephric-like adenocarcinoma of the female genital tract
Mesonephric carcinoma and mesonephric-like adenocarcinoma are rare aggressive neoplasms with overlapping morphological and immunophenotypic features. MC arises from mesonephric remnants, while MLA likely originates from Müllerian epithelium undergoing mesonephric differentiation.
World Health Organization reporting system for soft tissue cytopathology
The WHO Reporting System for Soft Tissue Cytopathology provides a standardized classification system to improve diagnostic accuracy and guide clinical decision-making. The system aligns with the WHO Classification of Soft Tissue and Bone Tumours and incorporates cytomorphologic features, ancillary studies, and clinical correlation.
Nanobody-based 3D immunohistochemistry allows rapid visualization in thick tissue samples
Researchers have developed a new 3D-IHC technique that improves the speed, depth, and sensitivity of immunolabeling in thick tissue samples using nanobodies fused with peroxidase. The method enables ultra-fast and high-resolution mapping of neurons and glia in 1-mm-thick brain tissues.
Study reveals how our gut cells detect harmful invaders
Researchers from Osaka University discovered that the GPR31 receptor in gut surveillance cells detects bacterial metabolites and triggers immune responses, which could lead to new drug developments and probiotic treatments. The study also found that these receptors play a key role in the immune response to gut infections in humans.
HER2-low and HER2-zero in breast cancer between prognosis, prediction and entity
Researchers discuss HER2 in breast cancer, highlighting the prognostic and predictive factors. They found an independent positive prognostic effect of HER2-low compared to HER2-zero in early breast cancer, challenging the long-held view that HER2-negative tumors do not benefit from trastuzumab-based therapy.
Oncotarget | Resistance of MMTV-NeuT/ATTAC mice to anti-PD-1 immune checkpoint therapy is associated with macrophage infiltration and Wnt pathway expression
Researchers found that MMTV-NeuT/ATTAC mice treated with anti-PD-1 therapy developed increased tumor-associated macrophages, EMT, fibroblast proliferation, and enhanced extracellular matrix. These findings suggest potential therapeutic avenues to enhance PD-1 immune checkpoint sensitivity.
Genes & Cancer | Systems biology network reveals the correlation between COX-2 expression and Ch 7q copy number alterations in Ch 11q-deleted pediatric neuroblastoma tumors
Researchers analyzed COX-2 levels and segmental chromosome aberrations in pediatric neuroblastoma tumor samples. Positive correlations between pre-CT Ch 7q gain and COX-2 expression were found, as well as negative correlations between Ch 7q gain and Ch 11q deletion.
Oncotarget | Expression of p-STAT3 and c-Myc correlates with P2-HNF4α expression in nonalcoholic fatty liver disease (NAFLD)
Researchers found that P2-HNF4α expression is associated with p-STAT3 and c-Myc expression in NAFLD patients, suggesting potential biomarkers for hepatocellular carcinoma (HCC) risk. Additionally, p-STAT3 expression was linked to hypertension, while c-Myc expression was associated with advanced fibrosis.
What makes mice fat, but not rats? Suppressing neuromedin U, study finds
A study by Okayama University researchers found that neuromedin U (NMU) is involved in metabolic processes but its 'anti-hunger' function may not be crucial for rats. Unlike mice, NMU expression is restricted in the rat brain and does not suppress feeding behavior.
Oncotarget | Glypican-3 (GPC3) is associated with MCPyV-negative status and impaired outcome in Merkel cell carcinoma
Researchers found that Merkel cell carcinoma (MCC) Glypican-3 (GPC3) is expressed in nearly 70% of MCC tumors and up to 90% of MCPyV-negative cases. GPC3 expression is associated with worse prognosis, including increased risk of death from MCC. This makes GPC3 a promising target for chimeric antigen receptor T cell therapy.
B.C. researchers develop new test to diagnose aggressive childhood brain tumours
Researchers at UBC and BC Cancer developed a new test to distinguish between high-risk medulloblastoma cases requiring radiation therapy and lower-risk cases. This test has the potential to improve diagnosis and future treatment of childhood brain tumours worldwide, reducing unnecessary side effects and increasing cure rates.
Varying immune cell levels in canine brain tumors could provide therapeutic targets
A new study reveals that high-grade gliomas in dogs contain more immune cells associated with suppressing immune response than low-grade gliomas. The findings suggest that these brain tumors may recruit cells to aid in immunosuppression, which could lead to improved immunotherapies for both humans and dogs.
Oncotarget: Heterogeneity of CEACAM5 in breast cancer
Researchers assessed CEACAM5 expression in breast cancer subtypes using immunohistochemistry, finding a high correlation between primary tumor and lymph node status. Expression of CEACAM5 also predicted different clinical outcomes depending on molecular subtypes, suggesting its potential role as a clinically relevant marker.
New radiomics model uses immunohistochemistry to predict thyroid nodules
A new machine learning-based model evaluates immunohistochemical characteristics in patients with suspected thyroid nodules, achieving excellent performance for individualized noninvasive prediction. The model improves thyroid nodule diagnoses and helps identify papillary thyroid cancers earlier.
Immunohistochemistry effectively detects ALK rearrangement
Researchers evaluate immunohistochemistry for detecting ALK rearrangement in lung adenocarcinoma, finding it a reliable and cost-effective method. The study suggests that IHC can effectively detect ALK rearrangement and identify suitable candidates for targeted therapy.
MARC Travel Awards announced for Immunohistochemistry and Microscopy (IHCM) Short Course 2013
The FASEB MARC Program conferred 11 travel awards totaling $20,350 to promote underrepresented minority students and scientists in immunohistochemistry and microscopy. The awards aim to increase diversity in biomedical research and encourage young scientists' participation.
Study shows immunohistochemistry is reliable screening tool for ALK rearrangement
A recent study found immunohistochemistry to be a reliable and accurate method for detecting ALK rearrangement in non-small cell lung cancer. The technique outperformed fluorescence in situ hybridization, which is currently the standard but time-consuming method.
Quick, easy test identifies aggressive type of lung cancer in never-smokers
A new test identifying aggressive lung cancer sub-type in never-smokers may help doctors select the most appropriate therapies. The study found that tumors expressing a protein product called anaplastic lymphoma kinase (ALK) are more common in this group, increasing disease progression risk.
JNCI May 12 issue tip sheet
A review of phase I clinical trial designs and the association between lymphovascular invasion and breast cancer recurrence, highlighting the need for new methods to expedite drug development and improve patient outcomes.
Immunohistochemical localization of Glutathione-S-transferase-pi in human colorectal polyps
The study found that GST-pi was highly expressed in neoplastic tissues and exhibited a significant increase in intensity with the grade of dysplasia. In addition, the research revealed unique ultrastructural changes in some cases characterized as mild-grade dysplasia.
Other highlights in the November 1 JNCI
Studies found that statins and fibrates have uncertain impact on melanoma prevention, while a new virus kills brain cancer cells in mice. An antibody combination shows efficacy in ovarian cancer, while immunohistochemical assays predict breast cancer treatment response better than older methods