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New RNA-based toolkit to regulate gene activity could advance regenerative medicine, gene therapy and biotechnology

Scientists developed a new RNA-based toolkit that can regulate gene expression, potentially improving regenerative medicine, gene therapy, and biotechnology. The technology uses small molecules to control the activity of synthetic RNA, allowing for precise control over gene expression.

SourceOkinawa Institute of Science and Technology (OIST) Graduate University·JournalJournal of the American Chemical Society·TypeExperimental study·DateMar 30, 2023

Nanotechnology enables visualization of RNA structures at near-atomic resolution

Researchers have developed a new approach to studying RNA molecules using nanotechnology and cryo-electron microscopy (cryo-EM), enabling the analysis of RNA subunits with unprecedented resolution. This breakthrough has significant implications for fundamental research, drug development, and RNA therapeutics.

SourceWyss Institute for Biologically Inspired Engineering at Harvard·JournalNature Methods·TypeExperimental study·DateMay 2, 2022

Unprecedented videos show RNA switching ‘on’ and ‘off’

Researchers at Northwestern University discovered a new mechanism called strand displacement, where RNA strands invade and displace each other to enable genetic expression. This finding has potential implications for designing successful drugs to target RNA-based diagnostics and treating illness and disease.

SourceNorthwestern University·JournalNucleic Acids Research·TypeComputational simulation/modeling·DateMar 28, 2022
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Uncovering the principles behind RNA folding

A Northwestern University research team discovered similarities in RNA folding among riboswitches, which could impact the design of future RNA-specific therapeutics and synthetic biology tools. The findings could also inform efforts to treat diseases triggered by RNA-level misfolding.

SourceNorthwestern University·JournalNature Chemical Biology·DateOct 21, 2019

Synthesizing chemical-sensing cells from scratch

Researchers at OIST and Osaka University develop an artificial cell system that interacts with histamine, a natural chemical compound. The system uses a riboswitch to turn on a gene inside the cells, which can eventually be used to release drugs in response to histamine signals.

SourceOkinawa Institute of Science and Technology (OIST) Graduate University·JournalJournal of the American Chemical Society·DateJul 2, 2019

Study finds snap-lock mechanism in bacterial riboswitch

Researchers deciphered the workings of a common bacterial switch that regulates protein production, revealing a snap-lock mechanism. This discovery could lead to new antibiotics as gram-positive bacteria use T-box riboswitches to regulate protein production.

SourceRice University·JournalNature Communications·DateMay 21, 2018
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How switches work in bacteria

Researchers analyzed the structure and dynamics of riboswitches using optical single-molecule experiments. They found that the riboswitch fluctuates between different conformations, with SAM attachment accelerating structure changes to ensure quick gene expression shutdown.

SourceKarlsruher Institut für Technologie (KIT)·JournalNature Chemical Biology·DateOct 12, 2017

The science of fluoride flipping

Researchers at UNC School of Medicine have developed a new imaging technique using nuclear magnetic resonance (NMR) to visualize RNA structure and motion over time. This discovery opens up new avenues for developing drugs that target RNA, crucial for understanding health and disease.

SourceUniversity of North Carolina Health Care·JournalNature Chemical Biology·DateAug 23, 2017

How researchers teach bacteria new behaviors

Researchers at Ruhr-University Bochum successfully taught bacteria to swim by combining various RNA modules in a new way. The team used riboswitches and RNA thermometers to control the bacterium's behavior and responded to temperature and metabolic products.

SourceRuhr-University Bochum·JournalNucleic Acids Research·DateApr 25, 2016
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Riboswitches in action

Researchers at SISSA have identified a key mechanism by which riboswitches regulate gene expression in bacteria. By using computer simulations to model the dynamics of the process, they found that binding to a metabolite molecule stabilizes the active form of the riboswitch, triggering protein synthesis.

SourceInternational School of Advanced Studies (SISSA)·JournalRNA·DateNov 4, 2013

Energy-sensing switch discovery could have broad implications for biology and medicine

Researchers at Scripps Research Institute have discovered a genetic sequence that can alter its host gene's activity in response to cellular energy levels, a finding that could have broad implications for biology and medicine. The energy-sensing switch, known as a riboswitch, detects the molecule ATP and controls global metabolic regul...

SourceScripps Research Institute·JournalNature Chemical Biology·DateOct 21, 2012

How bacteria fight flouride

Bacteria use riboswitches to detect and counteract the effects of fluoride, a key component of toothpaste. The discovery sheds light on how microbes overcome fluoride toxicity, potentially leading to new treatments for dental health issues.

SourceYale University·JournalScience·DateDec 22, 2011

Bacteria battle against toxic fluoride

New research reveals that many bacteria try to fend off fluoride by throwing it out, and that the presence of this transport system indicates fluoride has antimicrobial properties. The discovery also highlights a genetic switch called riboswitches, which can be used to enhance fluoride's effects against bacteria.

SourceHoward Hughes Medical Institute·JournalScience·DateDec 22, 2011

Scripps Research study sheds light on RNA 'on/off switches'

Scientists from Scripps Research Institute have discovered a new type of RNA molecule, called riboswitches, that can turn genes on or off in response to cellular energy needs. These findings may have implications for designing new antibiotics against harmful bacteria.

SourceScripps Research Institute·JournalNature Structural & Molecular Biology·DateFeb 13, 2011
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Genes under control

Max Planck scientists have successfully inserted a gene switch into the genetic material of chloroplasts in plant cells, allowing for controlled protein production. This breakthrough enables researchers to study the functions of chloroplasts and explore potential applications in biotechnology, such as producing antibiotics.

SourceMax-Planck-Gesellschaft·JournalProceedings of the National Academy of Sciences·DateMar 30, 2010

Scientists achieve first rewire of genetic switches

Researchers have achieved a breakthrough in regulating genes by hijacking riboswitches, opening doors for targeted drug discovery and synthetic biology. The study, published in Proceedings of the National Academy of Sciences, uses synthetic molecules to activate genes previously controlled by small naturally occurring molecules.

SourceUniversity of Manchester·JournalProceedings of the National Academy of Sciences·DateJan 25, 2010

Genetic switch potential key to new class of antibiotics

Researchers have identified a genetic mechanism in bacteria that could lead to the development of new antibiotics. The preQ1 riboswitch regulates gene expression by controlling the availability of queuosine, a molecule essential for bacterial survival and human disease.

SourceUniversity of Rochester Medical Center·JournalJournal of Biological Chemistry·DateApr 17, 2009
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Hairpins for switches

Researchers created hairpin-shaped RNA molecules that can differentiate between riboswitches in on and off states. These aptamers could help find new antibiotics by binding to the switches of pathogens, blocking essential protein synthesis.

SourceWiley·DateDec 12, 2006
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Switching to new anti-bacterial targets: Riboswitches

Riboswitches are RNA elements that control gene expression in essential metabolic pathways. Researchers at Yale University have identified pyrithiamine as a toxic compound that disrupts these pathways, leading to the development of new antibiotics.

SourceYale University·JournalChemistry & Biology·DateDec 16, 2005

Yale scientists find cooperative RNA switches in nature

Researchers at Yale University have identified a cooperative RNA switch in nature, which responds to various target compounds and regulates metabolic processes. This discovery supports the theory of an RNA World, where RNA molecules served a central role in early life.

SourceYale University·JournalScience·DateOct 7, 2004
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