Articles tagged with Short Hairpin Rna
Comprehensive exploration of living organisms, biological systems, and life processes across all scales from molecules to ecosystems. Encompasses cutting-edge research in biology, genetics, molecular biology, ecology, biochemistry, microbiology, botany, zoology, evolutionary biology, genomics, and biotechnology. Investigates cellular mechanisms, organism development, genetic inheritance, biodiversity conservation, metabolic processes, protein synthesis, DNA sequencing, CRISPR gene editing, stem cell research, and the fundamental principles governing all forms of life on Earth.
Comprehensive medical research, clinical studies, and healthcare sciences focused on disease prevention, diagnosis, and treatment. Encompasses clinical medicine, public health, pharmacology, epidemiology, medical specialties, disease mechanisms, therapeutic interventions, healthcare innovation, precision medicine, telemedicine, medical devices, drug development, clinical trials, patient care, mental health, nutrition science, health policy, and the application of medical science to improve human health, wellbeing, and quality of life across diverse populations.
Comprehensive investigation of human society, behavior, relationships, and social structures through systematic research and analysis. Encompasses psychology, sociology, anthropology, economics, political science, linguistics, education, demography, communications, and social research methodologies. Examines human cognition, social interactions, cultural phenomena, economic systems, political institutions, language and communication, educational processes, population dynamics, and the complex social, cultural, economic, and political forces shaping human societies, communities, and civilizations throughout history and across the contemporary world.
Fundamental study of the non-living natural world, matter, energy, and physical phenomena governing the universe. Encompasses physics, chemistry, earth sciences, atmospheric sciences, oceanography, materials science, and the investigation of physical laws, chemical reactions, geological processes, climate systems, and planetary dynamics. Explores everything from subatomic particles and quantum mechanics to planetary systems and cosmic phenomena, including energy transformations, molecular interactions, elemental properties, weather patterns, tectonic activity, and the fundamental forces and principles underlying the physical nature of reality.
Practical application of scientific knowledge and engineering principles to solve real-world problems and develop innovative technologies. Encompasses all engineering disciplines, technology development, computer science, artificial intelligence, environmental sciences, agriculture, materials applications, energy systems, and industrial innovation. Bridges theoretical research with tangible solutions for infrastructure, manufacturing, computing, communications, transportation, construction, sustainable development, and emerging technologies that advance human capabilities, improve quality of life, and address societal challenges through scientific innovation and technological progress.
Study of the practice, culture, infrastructure, and social dimensions of science itself. Addresses how science is conducted, organized, communicated, and integrated into society. Encompasses research funding mechanisms, scientific publishing systems, peer review processes, academic ethics, science policy, research institutions, scientific collaboration networks, science education, career development, research programs, scientific methods, science communication, and the sociology of scientific discovery. Examines the human, institutional, and cultural aspects of scientific enterprise, knowledge production, and the translation of research into societal benefit.
Comprehensive study of the universe beyond Earth, encompassing celestial objects, cosmic phenomena, and space exploration. Includes astronomy, astrophysics, planetary science, cosmology, space physics, astrobiology, and space technology. Investigates stars, galaxies, planets, moons, asteroids, comets, black holes, nebulae, exoplanets, dark matter, dark energy, cosmic microwave background, stellar evolution, planetary formation, space weather, solar system dynamics, the search for extraterrestrial life, and humanity's efforts to explore, understand, and unlock the mysteries of the cosmos through observation, theory, and space missions.
Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
A new biotechnical vector, VIBV, combines viral mimicry with synthetic nanotechnology to deliver targeted RNA therapies for cancer treatment. The vector uses a spindle-shaped nanostructure and polyethylene glycolylated liposomal coat to evade immunity and extend circulation.
Researchers at Northwestern University identified a new evolutionarily conserved RNAi-based form of cell death called Death Induced by Survival gene Elimination (DISE), which targets essential survival genes in cancer cells. This mechanism is ancient and effective against all cancers tested.
Researchers have discovered that targeting a specific mutation in fibrolamellar tumors can reduce tumor growth in mice, offering a promising approach to treating this nearly incurable cancer. The findings highlight the potential for novel therapies against an intractable disease.
Researchers from HKUST have identified secondary RNA elements that control DICER's cleavage activity, improving miRNA expression and shRNA design for effective gene silencing. The study provides a foundation for designing accurate and efficient short-hairpin RNAs.
Researchers at Toyohashi University of Technology have developed DNA stamper injections using nanoscale-tipped wire arrays to deliver biomolecules into live neuronal cells within brain tissues ex vivo and in vivo. This technique allows for the efficient genetic modification of brain cells, making it a powerful tool for neuronal research.
Researchers used a genetic screen to identify genes essential for neuron survival, including those involved in cellular metabolism. The study also uncovered new targets for treating Huntington's disease, such as the Nme gene family.
Researchers investigated shRNA therapy for Huntington's Disease, identifying novel methods to modulate construct expression and reduce off-target effects. The study proposes two feedback mechanisms to control shRNA expression, inspired by synthetic biology.
Scientists at CSHL have devised an algorithm that improves RNA interference technology harnessing short hairpin RNAs (shRNAs) for effective gene knockdown. The new algorithm, called shERWOOD, was trained on a massive parallel assessment of shRNA potency and can predict the efficacy of new sequences.
Researchers at Mirimus Inc. developed a new technology to enhance RNA interference efficiency and accuracy, enabling functional gene annotation in normal homeostasis and disease. The new approach uses an optimized microRNA backbone to increase the success rate of RNAi screens and models.
A recent study found that targeting the JAK1 and JAK2 tyrosine kinase pathways can increase tumor cell susceptibility to natural killer cell-mediated death. Pharmacological inhibition of these pathways was shown to enhance tumor cell killing, making them a promising target for cancer therapy.
Researchers developed a novel bipartite gene therapy approach to temporarily preserve photoreceptors in a mouse model of retinitis pigmentosa. The treatment targets defective phosphodiesterase metabolism, reducing cGMP and Ca2+ levels, and showing promise for treating this genetic disorder.
A CSHL-led team developed a powerful method to identify potent RNAi triggers, allowing biologists to fully exploit this natural mechanism. The approach revealed new insights into small RNA biogenesis and improved the recipe for creating potent RNAi triggers.
Research on heparanase-specific shRNA reveals its potential as a novel therapeutic strategy for human gastric cancer. The study successfully knocked down HPA expression in gastric cancer cells, leading to decreased invasiveness and metastasis. This finding suggests a new approach for treating cancers overexpressing HPA.
Researchers have identified a host of genes that cancer cells depend on for survival, including serine/threonine kinase 33 and polo-like kinase 1. Targeting these kinases could potentially lead to effective treatments for various types of cancer.
Researchers have developed a method to quickly identify shRNA that turns genes on and off, enabling complex genetic screens at minimal cost. The tool has the potential to revolutionize the study of gene function in mammals, paving the way for targeted therapeutics.
Researchers from MIT and Alnylam Pharmaceuticals have shown that siRNA does not interfere with the microRNA pathway, achieving 80% silencing of target genes in mice and hamster liver cells. This approach could lead to treatments for a wide range of diseases.
A human genome-wide RNAi library has been developed by Cold Spring Harbor Laboratory, enabling companies to identify and validate target genes for new drugs. The library targets over 10,000 human genes with sequence-validated short hairpin RNA molecules.
Researchers have created new RNA libraries that can selectively inactivate human genes, enabling efficient screening for genetic defects. The libraries, made widely available to the research community, will greatly aid in understanding human biology and disease.