Immunohistochemical Staining

Kumamoto University technical expert receives prestigious MEXT Minister’s Award for excellence in research support

Nanobody-based 3D immunohistochemistry allows rapid visualization in thick tissue samples

Researchers have developed a new 3D-IHC technique that improves the speed, depth, and sensitivity of immunolabeling in thick tissue samples using nanobodies fused with peroxidase. The method enables ultra-fast and high-resolution mapping of neurons and glia in 1-mm-thick brain tissues.

Mapping of circadian clock-related neuron connections in Drosophila

Researchers have constructed a comprehensive map of synaptic connections between clock neurons in Drosophila, identifying novel neurons and connections that influence feeding, mating, and sleep behaviors. The study also reveals the role of light input pathways and neurotransmitters in regulating clock neuron function.

Expression of carbonic anhydrase IX as a novel diagnostic marker for differentiating pleural mesothelioma from non-small cell lung carcinoma

This study investigates the use of carbonic anhydrase IX (CAIX) as a diagnostic marker for distinguishing pleural mesothelioma from non-small cell lung carcinoma (NSCLC). CAIX expression is found to be highly sensitive and specific in detecting pleural epithelioid mesothelioma, comparable to or better than existing mesothelial markers.