Articles tagged with Live Cells
Researchers discovered a new immune tolerance mechanism that relies on blocking Signal Two coupling, rather than expanding it. This finding holds promise for developing new therapies against inflammatory bowel disease and other autoimmune conditions.
A study identified exosomal microRNA-122-3p as a key driver of MASLD pathogenesis. Elevated miR-122-3p levels induced triglyceride accumulation and reactive oxygen species production in liver cells.
Researchers at Colorado State University used AI to modify antibodies into stable intrabodies that can visualize histone modifications in real-time. This allows for better understanding of gene expression and its relationship with cancer and other disorders. The team created 19 new antibody-based probes with a 70% success rate, signifi...
Researchers at the University of Houston have discovered a potential therapeutic strategy for counteracting muscle wasting in pancreatic cancer by blocking a specific cell pathway. Muscle wasting, also known as cachexia, is a debilitating syndrome affecting 60-85% of patients with pancreatic cancer.
Researchers have created a method for simultaneous imaging of DNA and RNA in living cells using harmless infrared light, allowing for high-precision detection of all stages of cell death. This breakthrough enables the early detection of cellular damage that leads to aging or death.
Cryo-optical microscopy captures high-resolution, quantitatively accurate snapshots of dynamic cellular processes at precisely selected timepoints. This technique enables the observation of transient biological events with unprecedented temporal accuracy.
Researchers have identified a previously unknown organelle called the hemifusome that plays a crucial role in cellular sorting and recycling. This discovery could lead to targeted treatments for complex genetic disorders like Hermansky-Pudlak syndrome, which affects multiple systems in the body.
A study at the University of Zurich tracks live cellular development and epigenetic changes over multiple generations, showing how stress induces heterogeneity and increases genetic complexity. This research may lead to better understanding of cancer cell diversity and develop more effective therapies.
A new universal photocage modification strategy based on thioketal enables real-time live cell subcellular imaging. The thioketal-based probe SiR-EDT exhibits improved dark stability and can be specifically activated by UV-visible light.
A new study demonstrates how fluorescent cholesterol probes can visualize cholesterol in live cells, revealing its role in amyloid plaque formation and cellular signaling. The novel probes have the potential to enhance our understanding of how cholesterol imbalances contribute to neurodegenerative disorders.
Researchers from Brookhaven National Laboratory have developed an effective way to image a single cell using multiple techniques, providing significant implications in medicine and agriculture. The team used advanced X-ray imaging technologies to capture high-resolution images of the cellular structure and chemical processes within cells.
Researchers question whether micronuclei activate the cGAS-STING pathway, a key innate immune response to foreign nucleic acids. The study found that MN more commonly recognizes DNA during cell division without triggering STING activation.
A new method for phase-modulated stimulated Raman scattering tomography enables rapid, label-free 3D chemical imaging of live cells and tissues. This technique improves lateral resolution and imaging depth compared to conventional methods.
Researchers have developed a proof-of-concept technique to attach nanomaterials to living tissue and cells using nanoimprint lithography. The method enables biocompatible integration of smart devices with living tissue, opening up opportunities for biohybrid materials, bionic devices and biosensors.
Researchers developed a new probe to measure pH levels in cells, revealing a constant conversion rate from endosomes to lysosomes. The probe's ability to track pH changes enables faster diagnosis and potential treatments for lysosomal diseases.
A new glycosidic-bond-based mass-spectrometry-cleavable cross-linker has been developed to improve data analysis throughput and identification accuracy of cross-linking information. This technique enables in-vivo cross-linking of protein complexes in live cells, achieving large-scale and precise analysis of 1,453 proteins.
Researchers discovered that lipid deposition on medical implant surfaces can signal to the immune system whether to attack or ignore the implant. This knowledge could help develop biomaterials that deflect host immune aggression, reducing malfunction rates for devices like pacemakers and surgical mesh.
The study uses 3D electron microscopy and live cell imaging to observe molecular tethers between organelles. These dynamic structures are constantly binding and unbinding, altering contact site configuration in response to cellular changes.
Researchers develop hybrid brightfield-darkfield transport of intensity approach, expanding accessible sample spatial frequencies and achieving 5-fold resolution increase. This method enables precise detection and quantitative analysis of subcellular features in large-scale cell studies.
Researchers developed a mathematical model to predict the efficiency of nanoparticle delivery into cells, particularly in stem cells. They found that nanoparticles become trapped in bubble-like vesicles, preventing them from reaching their targets.
Scientists at the University of Bath have developed a new technique called Transcription Block Survival (TBS) to accelerate the discovery of cancer-fighting drugs. TBS identifies molecules that can shut down dangerous proteins before they wreak havoc, by blocking their interaction with cell DNA.
Researchers have discovered live cells in human breast milk, which may provide insights into breast cancer development and improve breastfeeding outcomes. The study, led by Dr. Alecia-Jane Twigger, found that these living cells can be used to study mammary gland function and identify potential early indicators of future breast cancer.
A flexible and easy-to-use micropen setup is capable of directly writing on surfaces to a microprecise level. The device allows for the printing of microarrays, lines, curves, and other structures in real-time using biomaterial or conductive ink.
Scientists have developed a method to synthesize nanocrystals in live cells through space-time coupled synthesis, enabling the creation of super biosystems. This approach has been successfully applied to various cell types, including yeast, bacteria, and mammalian cells.
Researchers develop a de novo peptide Y15 that readily forms secondary structures to enable bottom-up synthesis of functional protein assemblies in live cells. The peptide enables the formation of fibrous structures and clusters in test tubes and live cells, facilitating protein assembly and reconstitution of natural complexes.
Researchers demonstrate simultaneous imaging of up to 6 subcellular targets with low crosstalks and high temporal resolutions. They achieve full-frame high sensitivities in quantifying mitochondrial matrix pH and intracellular macromolecular crowding.
Researchers create a microfluidic probe that generates free radicals with controlled size and concentration, allowing them to manipulate small areas on cellular surfaces. This approach enables the study of cell reactions to radicals in a controlled way, opening up new possibilities for subcellular research.
Researchers developed a new method combining label-free imaging with artificial intelligence to study live cells over time. The technique allows for the estimation of cell attributes without using toxic fluorescent dyes.
Researchers have developed a method to label and image cell surface receptors on live cells with two different colors, allowing for the study of receptor dynamics and pharmacology in their native setting. This innovation expands the possibilities for studying G-protein coupled receptors and other important drug targets.
Scientists developed SR-FACT microscopy, combining label-free ODT and two-dimensional fluorescence microscopy to visualize cellular environment. The technique revealed novel subcellular structures like dark-vacuole bodies interacting with organelles.
The team developed a new chemical tool to reveal the topology of IMM proteins in live cells, confirming 58 topologies and determining 77 previously uncharacterized ones. This breakthrough will help speed the development of mitochondria-targeted therapeutics for various human metabolic diseases.
A new imaging technique enables researchers to visualize chromatin's dynamic processes in live cells, revealing its organization and response to stimuli. This breakthrough offers insights into the complex relationship between chromatin and gene expression, with potential implications for understanding cancer development.
Scientists at UMass Chan Medical School discovered how CRISPR-Cas9 targets DNA in live cells, finding that guide RNA stability affects cleavage. The study's findings help predict off-target cuts and may improve gene editing tools.
Researchers at Université de Genève have developed new tools to manipulate biology, including a novel co-factor that enables proteins to perform tasks previously thought impossible. Meanwhile, another team has created a method to visualize mRNA in live animals, providing real-time insights into cellular processes.
Researchers have developed a new technology using CRISPR/Cas9 to label and track up to seven different genomic locations in live cells. This allows for real-time study of chromosome dynamics and gene expression, enabling better understanding of biological processes and health outcomes.
Researchers have developed a novel technique to detect cell toxicity in real-time during drug screening, using DNA-binding fluorescent dyes. Four dyes were identified as safe and impermeable to cells, enabling the detection of cell death during high-throughput screening.
Researchers from Clemson University have developed a method to create temporary holes in the membranes of live cells using a standard inkjet printer. This allows them to introduce molecules inside the cells that wouldn't otherwise fit, enabling studies on cell mechanics and responses to mechanical forces.
PNNL has developed power-delivery devices to improve trace gas analysis and create propylene glycol from renewable sources. The lab's IncubATRTM technology enables real-time monitoring of live cells, accelerating scientific discovery and reducing animal testing.
Berkeley researchers have created a copper-free version of click chemistry, allowing for the first time to label and image glycans, proteins, and lipids in live cells. The technique, developed by Carolyn Bertozzi and her team, proceeds at physiologically acceptable temperatures without toxic copper catalysts.