Articles tagged with Plasmids
A new study reveals that bacteria can actively limit the spread of antibiotic resistance genes by deploying molecular gatekeepers called YokF. This mechanism blocks the transfer of beneficial genes, giving microbes a competitive advantage in dense microbial communities.
Researchers at Tokyo Metropolitan University have created a neutral molecule that can carry DNA into biological cells using a process called annealing. This breakthrough promises more effective therapies by reducing inflammation and improving delivery efficiency.
Scientists have developed a new strategy to combat antibiotic resistance by studying the competition among plasmids within bacterial cells. By isolating individual cells and measuring intracellular plasmid competition, researchers discovered basic properties of plasmid and bacteria fitness and evolution.
Researchers have identified a minority of plasmids as the primary cause of multidrug resistance in bacteria, evolving to gain resistance through selective pressure from antibiotics. The study developed a model for plasmid evolution, highlighting pathways and predicting future outbreaks.
Researchers at North Carolina State University have developed a controlled evolution technique that dramatically increases plasmid DNA (pDNA) production in E. coli bacteria. This breakthrough could significantly reduce the cost of gene therapies and expedite research, making pDNA resources more accessible.
Researchers at the University of Birmingham have identified a genetic code that enables the displacement of antibiotic resistance genes from bacteria. This discovery could lead to the development of new probiotics to combat antibiotic resistance in both animals and humans, making them reservoirs of this issue.
A new study found that recombinant adeno-associated virus (rAAV) capsids contain single-stranded DNA impurities derived from plasmid and host cell DNA. The researchers suggest that the adverse effects of these impurities may differ from those of double-stranded DNA, highlighting the need for further evaluation.
A new study finds that Enterococcus faecalis produces protective capsular slime when present with certain strains of pathogenic Escherichia coli, making E. coli more resistant in low-iron environments. This discovery could lead to the development of targeted therapies for specific dog and poultry infections.
Researchers discovered a bacterial defense strategy involving two proteins that team up to disable plasmids, which could be applied to gene editing. Guide DNA and a functional protein are key components of this system, showing promise for targeted genome editing.
A mysterious plasmid, pBI143, found in 90% of human intestines, could be used to identify faecal contamination and offer insights into intestinal diseases. The discovery also highlights the prevalence of 'cryptic' plasmids in human gut microbiota.
A new compartment in mammalian cells, the exclusome, has been discovered to house DNA rings that can be ejected from the nucleus. This process helps protect chromosomes from foreign DNA that could disrupt cellular function. The discovery sheds light on a potential link between the exclusome and autoimmune diseases.
Researchers have discovered a new plasmid in epidemic Vibrio cholerae samples that introduces genes encoding resistance to multiple antibiotics. The finding underscores the importance of genomic surveillance and suggests that the strain's stability poses a concerning factor for future outbreaks.
A new computational technique analyzes bacterial genetic sequences to monitor the spread of antibiotic resistance over time. The study found that resistance genes most likely to spread are those on conjugative plasmids and targeting specific antibiotics, with many coming from a single source.
Researchers discovered that plasmids can linger in the nose of lab workers for weeks, interfering with clinical diagnostic tests. The study highlights the importance of considering occupational exposure in diagnosis and treatment.
Researchers have developed a novel COVID-19 vaccine based on altered plasmid DNA that effectively blocks cell infection across all tested variants. The vaccine targets a specific vulnerability in the SARS-CoV-2 virus's spike protein, inducing a focused antibody response.
A research team from Tokyo University of Science has developed a new method to create copolymers with different metal species, which have potential uses in catalysis and drug discovery. The technique allows for controlling the composition of metal species in the resulting polymer.
Researchers at University of Illinois at Urbana-Champaign have developed PlasmidMaker, an automated platform for designing and constructing plasmids. The platform uses Pyrococcus furiosus Argonaute-based artificial restriction enzymes to assemble DNA fragments with greater flexibility and precision.
Biomedical engineers at Duke University have discovered a physical mechanism that causes high doses of antibiotics to promote the spread of antibiotic resistance between bacteria. The culprit is an overabundance of 'jumping genes,' called transposons, which carry genetic instructions for resistance from cell's source code to plasmids.
Researchers at the University of Copenhagen have discovered that resistant bacteria can hide resistance genes in inactive bacteria within biofilms, creating a reservoir of resistance that can be drawn upon when antibiotics are not present. This new understanding challenges the long-held assumption that resistant bacteria lose their res...
Researchers have created DNA-based materials with tunable properties, which can be controlled by adjusting the level of supercoiling. These materials have potential applications in drug delivery and tissue regeneration.
Kytai Nguyen's research aims to reduce complications and improve quality of life for PAD patients by delivering plasmids that protect cells and promote new blood vessel growth. The project has the potential to offer hope to millions worldwide affected by this common malady.
Researchers at the University of Washington and Idaho discovered that prolonged antibiotic exposure can 'prime' single-resistant bacteria to become multidrug-resistant. This effect makes it more likely for bacteria to acquire resistance to multiple antibiotics, even in an antibiotic-free environment.
A new strain of Klebsiella pneumoniae was discovered in 2017 and found to be resistant to carbapenem antibiotics. A recent study identified an easily transmitted DNA piece that can make this superbug even more deadly and hyper-virulent, posing a significant threat to human health.
Scientists have discovered a new mobile DNA element in the mosquito parasite Wolbachia, which may contribute to improved control strategies for mosquito-borne illnesses. The novel plasmid is widespread across natural Wolbachia populations and has potential implications for controlling disease transmission.
Researchers analyzed over 400 anthrax strains and found that human and animal samples tend to have more virulent plasmids than environmental sources. The study suggests a correlation between plasmid copy number and virulence, offering new insights into the severity of specific anthrax strains.
Addgene has made over 100,000 CRISPR plasmids available to laboratories worldwide, democratizing genome editing research. This global access has enabled the advancement and flourishing of CRISPR technology.
Researchers found plasmids, small DNA molecules that replicate independently, in Antarctic haloarchaea microbes. These plasmids can masquerade as viruses and infect other cells, potentially evolving into protective coats for successful virus invasion.
Researchers at Penn State have created inexpensive molecular rulers for DNA research using new, license-free DNA ladders. These plasmids can estimate DNA fragment sizes between 50 and 5,000 base pairs in length, offering a cost-effective alternative to commercially available methods.
Researchers at the Wyss Institute for Biologically Inspired Engineering have developed a novel, inexpensive method for detecting the Zika virus that could help slow its spread. The system uses a simple modular workflow comprising three steps: amplification, detection, and strain identification.
Researchers found that plasmid DNA attached to a rocket exterior survived suborbital spaceflight, re-entry, and landing conditions. The study showed that up to 53% of the DNA retained its full biological function, with 35% remaining functional after heating up to 1000°C.
Researchers from China Medical University discovered that overexpressing the cytoglobin gene in SH-SY5Y cells enhances their resistance to cobalt chloride-induced hypoxia. This breakthrough finding has significant implications for developing gene therapy treatments for hypoxic-ischemic neurological diseases.
A research team at UNC Chapel Hill has discovered a molecular mechanism that allows bacteria to transfer antibiotic resistance. By inhibiting the interaction between two protein loops and the plasmid DNA, researchers may be able to develop new antibiotics effective against drug-resistant bacteria.
Scientists have made significant progress in developing effective delivery systems and efficient biomanufacturing strategies for DNA vaccines. Novel methodologies have emerged, including transdermal patches, electroporation, and plasmid purification techniques.
Researchers have developed an artificial plasmid coding for Sindbis virus replicase genes that causes regression of lung and melanoma tumors in mice. The plasmid forces cells to produce double-stranded RNA, leading to cell death and activation of immune responses.
Researchers found that biological clocks influence gene activity by controlling chromosome coiling in cyanobacteria, suggesting a universal theme for higher organisms. The study provides direct evidence of the regulatory mechanism, which could explain why some genes are active during the day and night.
Researchers have developed a new classification system for Rickettsia bacteria, highlighting the importance of plasmids in virulence traits and host recognition. The study provides insight into the evolutionary origin of rickettsiae and has implications for the development of diagnostics and vaccines.
Scientists have developed a stripped-down gene therapy approach using naked DNA to treat rheumatoid arthritis in rats. The treatment, which injected TGF-ß plasmids into muscle tissue, significantly reduced chronic arthritis symptoms and joint inflammation.