Articles tagged with Single Stranded Dna
Researchers have captured the most detailed structural images to date of a specific type of protein's DNA repair process, revealing key steps in its repair activities. The findings provide insights for drug targets that could halt the process in cancer cells empowered by mutated BRCA genes.
A new study found that recombinant adeno-associated virus (rAAV) capsids contain single-stranded DNA impurities derived from plasmid and host cell DNA. The researchers suggest that the adverse effects of these impurities may differ from those of double-stranded DNA, highlighting the need for further evaluation.
Researchers have discovered a new mechanism of how anticancer drugs attack and destroy BRCA mutant cancer cells, including drug-resistant breast cancer cells. The study found that small DNA nicks can expand into large single-stranded DNA gaps, leading to cell death.
PARP inhibitors have been found to be effective in treating cancers with BRCA1/2 mutations by blocking DNA repair pathways. The combination of PARPis with chemotherapeutic drugs can also improve treatment efficacy, increasing DNA damage and blocking repair processes.
Researchers at Tokyo Institute of Technology developed a method to precisely control the timing of DNA droplet division, mimicking biological Liquid-Liquid Phase Separation (LLPS) droplets. This breakthrough enables precise control over synthetic droplet dynamics, key to developing bio-inspired systems.
The study demonstrates significant advancements in stability and functionality of ssDNA-SWCNT complexes, with high-affinity sequences showing superior binding strength. The findings also reveal notable improvements in resistance to enzymatic degradation, making these complexes suitable for long-term biological applications.
When E. coli detects damage from antibiotic Ciprofloxacin, it sends out an SOS signal that alters cellular activity. The bacteria then mutate their DNA to repair the damage or adapt to resist the antibiotic. Researchers studied this process in detail using bioreactors and found all genes are activated simultaneously at the protein level.
Researchers developed a new technique called HiDEF-seq to detect early molecular changes in DNA code that precede mutations. The study found higher numbers of single-strand DNA changes in healthy cells from people with genetic syndromes linked to cancer, suggesting a link between these changes and the development of cancer.
Researchers have discovered a virus that infects the fungus Batrachochytrium dendrobatidis, which causes heart failure in frogs and toads. The virus could be engineered to control the fungal disease and potentially save amphibian species.
Scientists have discovered two new end-replication problems in DNA replication, affecting both the leading and lagging strands. This revelation changes our understanding of telomere biology and may hold clinical implications for individuals with telomere disorders, such as Coats plus syndrome.
Researchers have mapped hundreds of pig genes and identified similarities with humans, shedding light on disease development and potential treatments. The study paves the way for targeted medicines and more precise gene editing in pigs.
Scientists have introduced a new class of protease-activity sensors using gold nanoparticles equipped with peptide DNA, which can detect multiple active proteases in parallel. The method works at room temperature and does not require complicated sample preparation or elaborate instruments.
Researchers identified key factors in DNA repair, revealing the 'proofreading' portion of polymerase epsilon helps prevent strand breakage. This knowledge arms scientists with ways to enhance anti-cancer drug effectiveness and develop new diagnostic methods.
Researchers discovered NSMF protein's role in alleviating DNA replication stress by displacing weakly bound RPA proteins and promoting phosphorylation. This mechanism accelerates relief of replication stress, offering a new direction for treating various diseases, including cancer and age-related conditions.
CyDENT base editors allow efficient and precise modification of genetic information in living organisms. The system enables strand-specific base editing in nuclear and organellar genomes, with high strand specificity demonstrated in mitochondrial genome editing.
Researchers at the University of Missouri have developed a new method using nanopores to advance discoveries in neuroscience and medical applications. The technique allows for real-time detection of dynamic aptamer-small molecule interactions, which can aid in understanding DNA and RNA diseases and drug discovery.
Researchers at Weill Cornell Medicine have discovered a DNA molecule that folds into a four-way junction structure, allowing it to mimic the activity of green fluorescent protein (GFP). This breakthrough could lead to the development of new DNA-based fluorescent tags for rapid-diagnostic tests and various scientific applications.
A new test developed by Cambridge researchers uses single strands of DNA as 'bait' to detect multiple respiratory viruses, including influenza, rhinovirus, RSV, and COVID-19, with highly accurate results in under an hour. The test has the potential to improve patient outcomes and reduce the use of antibiotics.
Researchers at Gladstone Institutes and UCSF have developed a new approach to introduce long DNA sequences into cells with remarkable efficiency. The technology, which uses single-stranded DNA templates, overcomes the limitations of traditional viral vectors and has the potential to make cell therapies faster, better, and less expensive.
Researchers have modelled a key mechanism by which DNA replicates, revealing details about how helicases wrangle DNA during replication. The simulations showed each step of translocation can travel more than 12 nucleotides along the backbone, pinpointing interactions involved in long-distance movement.
Researchers developed a simple physical model to explain DNA deformations caused by ions and temperature changes. The model reveals that salt-induced twist changes are driven by electrostatic interactions, while temperature-induced changes are related to DNA diameter variation. These findings provide new insights into the molecular mec...
Researchers developed a MOF-based system for delivering DNA into target cells, overcoming challenges in gene therapy. The tiny structures protected genetic cargo and helped ferry it into the nucleus, where gene activity takes place.
Researchers develop DNA Nanoswitch Calipers to measure distances within single molecules using force, enabling the identification of single proteins in samples. This technique creates a unique 'fingerprint' that can be used to identify known molecules or infer structural information about unknown ones.
Researchers at IOCB Prague have created a glowing DNA enzyme called Supernova, which catalyzes a chemiluminescent reaction. This breakthrough uses artificial evolution to identify light-producing deoxyribozymes in a vast library of DNA molecules, opening up new possibilities for point-of-care assays and high-throughput screens.
Researchers at Jiangnan University developed a sensing platform to detect microRNA-205, a potential biomarker for radiation-resistant nasopharyngeal carcinoma. The method shows high sensitivity and excellent selectivity, enabling detection of miR-205 with a limit of detection of 4.78 nM.
The study optimized pegRNA designs to maximize plant prime editing efficiency, finding that the melting temperature of the PBS sequence is crucial for efficient editing. The introduction of dual pegRNAs resulted in significant improvements in editing efficiency, with a 3.0-fold increase compared to individual pegRNAs.
Researchers use a scanning tunneling microscope to study DNA hybridization, monitoring changes in electronic properties of single molecules. They discovered plateaus in current traces indicating the formation of double-stranded DNA, providing new insights into chemical reactions and potential applications for DNA-based diagnoses.
Researchers at Arizona State University have developed a new type of meta-DNA structure that can be used to engineer sophisticated nanoscale structures and devices. The meta-DNA self-assembly concept has opened up new possibilities for optoelectronics, including information storage and encryption, as well as synthetic biology.
A DNA twist separates double helix into a single strand, regulating placental growth and development. The sixth base of DNA, N6-methyladenine, stabilizes this structure, controlling cell fate.
Researchers have discovered that cancer cells can tolerate high amounts of single-stranded DNA, which is a common sign of stress during cell division. By inhibiting the POLA1 gene, cells can be made to crash when they divide, potentially leading to new cancer treatments.
Researchers at IBS discover sequence-dependent information influences liquid-liquid phase separation. Single-stranded DNA forms droplets easily, while double-stranded DNA requires specific conditions due to its rigid structure.
Researchers at Arizona State University have developed a method to create complex knot-like nanostructures in single-stranded DNA, with crossing numbers ranging from 9 to 57. This breakthrough enables the design of molecular structures with specific functions and unprecedented complexity.
A team of Polish-American-Italian researchers has successfully created a record-long polymer DNA negative, featuring a sequence with all four nucleobases. The synthetic molecule functions chemically like a normal strand of deoxyribonucleic acid and demonstrates improved properties over natural DNA.
Researchers developed the DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) system, allowing quick detection of diseases such as HPV using Cas12a. The system involves adding reagents in one reaction and uses isothermal amplification to boost target DNA cuts, resulting in a fluorescent readout.
Researchers have created large-scale, programmable self-folding DNA and RNA structures using user-friendly software tools. The new nanostructures can be successfully cloned and amplified inside living cells, offering a low-cost high-scale production strategy for manufacturing the nanostructures.
Scientists develop single-stranded DNA origami, a breakthrough in nanotechnology that can create complex structures without knots. The technology has potential applications in medicine, including delivering drugs inside cells.
Researchers develop a single-strand DNA robot that can autonomously pick up and sort molecules into distinct regions on a surface. The robot successfully sorted six scattered molecules in 24 hours, and its design can be generalized to work with dozens of types of cargos.
A Berkeley Lab-led study reveals phosphate steering, an electrostatic force, guides key enzyme used by both healthy and diseased cells. This finding provides new directions for cancer treatment research.
Researchers have developed a new method using terminal deoxynucleotidyl transferase (TdT) enzyme to produce precise, high molecular weight synthetic biomolecular structures. These structures can be tailored to create single-stranded DNA for self-assembling into ball-like containers for drug delivery or incorporating unnatural nucleotides.
The study reveals a left-handed coil structure of the Mcm2-7 hexamer and Cdt1-MCM heptamer, shedding light on DNA unwinding mechanisms. The open-coil structure has profound implications for understanding DNA replication initiation and elongation.
A recent study by Caltech and Vanderbilt University researchers found that electrons play a crucial role in DNA replication, allowing the cell to quickly locate and repair mutations. The discovery reveals a new pathway for cells to regulate DNA replication, which is essential for maintaining genome stability.
Researchers have characterized the critical function of the Zf-GRF domain in manipulating DNA during repair processes. The domain is essential for APE2 enzyme activity, enabling it to bind to single-stranded DNA and facilitate its 3'-5' resection.
A new diagnostic tool uses short-strand DNA library preparation to analyze cell-free DNA in plasma, enabling transplant recipients to get an idea of how their new organ is responding via a simple blood test. This method can help determine whether the transplanted organ is injured or being rejected.
Researchers have designed DNA frames to connect nanoparticles into precisely structured lattices, enabling the creation of nanomaterials with tailored properties. The team's method uses DNA origami to self-assemble particles into desired shapes, reducing dependence on particle modification.
A new method for designing geometric forms built from DNA has been developed, allowing for the creation of tiny structures in 2 and 3 dimensions. The technique, known as DNA origami, relies on a top-down strategy and can produce virtually any polyhedral shape.
A team of Swiss and Russian scientists has deciphered how APOBEC takes advantage of a weakness in DNA replication to induce mutations, primarily affecting early-replicating genes. The study reveals that APOBEC targets single-stranded DNA regions during replication, which are more prone to mutations.
Researchers created bundles of double-helix molecules and used them to form a rigid framework, then added complementary strands to glue nanoparticles in place. This method produced predictable clusters and arrays with tailored structures and functions.
University of Texas Medical Branch researchers have figured out how mammalian cells repair damaged bases in the single-stranded genome. The 'cowcatcher' enzyme, NEIL1, rides in front of the replication complex to scout for damage and stalls machinery until it's repaired.
A new method for manufacturing short, single-stranded DNA molecules has been developed by researchers at Karolinska Institutet and Harvard University. This technique can produce large amounts of DNA copies cheaply using bacteria, improving the quality and scalability of DNA fragment production.
Researchers at ASU's Biodesign Institute have developed novel 2-D and 3-D DNA nanotechnology structures that push the boundaries of complex molecular designs. By re-engineering the Holliday junction, they created flexible geometries for building in three dimensions, enabling multilayered and curved objects.
Researchers led by NUS Associate Professor Yan Jie identify three new distinct overstretched DNA structures caused by mechanical stretching, resolving a long-standing scientific debate. The discovery has implications for understanding DNA damage repair and gene transcriptions, with potential applications in designing new DNA devices.
Researchers at National University of Singapore have identified a novel double-stranded DNA structure, dubbed S-DNA, which has sparked a 16-year scientific debate. The team's findings suggest that S-DNA may be a potential binding substrate for DNA intercalators and proteins.
Scientists at the University of California, Davis have made a significant discovery on how DNA repairs itself. They found that the protein Rad51 searches for the correct region to use for repair by forming an extensive filament and guiding it to the right place in the chromosome.
Researchers at Arizona State University have developed a method to construct arbitrary, two and three-dimensional shapes using DNA origami. The new technique allows for the creation of complex curvature in 3D nanostructures, enabling potential applications in ultra-tiny computing components and nanomedical devices.
A new JACS paper demonstrates continuous and controlled translocation of a single-stranded DNA polymer through a protein nanopore using a DNA polymerase enzyme. This achievement advances the development of Strand Sequencing using nanopores, a crucial component of molecular motors.
Researchers at UCSC and Oxford Nanopore Technologies Ltd demonstrate fine control of DNA translocation through a protein nanopore using electronic feedback. This breakthrough advances towards direct, electrical detection and analysis of single molecules for various applications, including DNA sequencing.
Researchers at Harvard Medical School have created nanodevices made of DNA that can self-assemble and be programmed to move and change shape. These programmable nanodevices are highly suitable for medical applications due to their biocompatibility and biodegradability.
Researchers used DNA origami to create molecular breadboards, solving the challenge of organizing carbon nanotubes into nanoscale electronic circuits. The innovative technique exploits DNA's sequence-recognition properties and stickiness to 'stick' carbon nanotubes onto its surface.
A new study reveals that a single-stranded DNA-binding protein (SSB) moves back and forth along single-stranded DNA, gradually allowing other proteins to repair, recombine or replicate the strands. SSB's dynamic movement is independent of the DNA sequence and modulates the activity of critical DNA repair proteins.
Researchers at the University of Illinois have discovered new deoxyribozymes capable of cleaving single-stranded DNA with sequence and site selectivity. These DNA catalysts require two metal ions and hold promise for developing more efficient methods for manipulating DNA.