To illustrate the utility of their MORF collection, the group used it to make a protein chip containing 5573 purified proteins and performed the first global analysis of protein glycosylation. They identified 109 new N-linked glycoproteins - nearly doubling the known yeast glycome.
"The C-terminal tag allows expressed proteins to enter the secretory pathway normally, which is important for proper post-translational modification and function of the proteins. The discovery of over 100 glycoproteins in a single pass illustrates the power of screening an entire proteome at once."
Genes & Development