HLA-G gene harbors certain polymorphic variations that can potentially impact its biological activity, and therefore, may confer a risk for recurrent pregnancy loss (RPL). This study aimed to analyze whether HLA-G polymorphic variations ( G*0103, G*0104 , and G0105N ) are related to the risk of RPL in women from Kashmir, North India.
A total of 200 women who suffered ≥2 RPLs and 240 healthy controls were recruited from the same geographical region. Additionally, 100 spouses of RPL affected women and 60 products of conception were evaluated. HLA-G genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method.
The variant genotype 0103:0103 in exon 2 of HLA-G was not detected. The genotype 0104/0105 was detected in 100% of RPL patients, spouses, and controls. Exon 2 and variant genotypes G*0103 in exon 2 and G*0105 in exon 3 of HLA-G were absent in our population and thus did not contribute to the etiopathogenesis of RPL. In contrast, the exon 3 HLA-G variant G*0104N was significantly more frequent in RPL patients and their spouses compared to the control group (p<0.05). The presence of the HLA-G variant genotype G*0104N (exon 3) was detected in 13% of RPL patients and 7% of their male partners, indicating a significantly higher frequency than in controls and suggesting a substantial risk for RPL (p<0.05).
In conclusion, the presence of the HLA-G *0104 allele at a higher frequency in both partners strongly indicates a significant risk for RPL within our population. We further conclude that there is no role for HLA-G *0103 and *0105 in our population.
https://www.xiahepublishing.com/1555-3884/GE-2023-00144
The study was recently published in the Gene Expression .
Gene Expression (GE) is an open-access journal. It was launched in 1991 by Chicago Medical School Press, and transferred to Cognizant Communication Corporation in 1994. From August 2022, GE is published by Xia & He Publishing Inc.
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Gene Expression
HLA-G Polymorphic Variation (G*0104N exon 3) Confers Potential Risk for Recurrent Pregnancy Losses: A Study in a High Incidence Zone (Kashmir, North India)
12-Jun-2024