A long-term field study conducted in Northeast China’s fertile black soil region has demonstrated that biochar can significantly improve soil health, stabilize microbial communities, and increase crop yields—but only when applied at the right rate.
The research, published in Biochar , reveals that a one-time application of biochar at 31.5 metric tons per hectare (t ha⁻¹) led to higher soil organic matter stability, enhanced bacterial diversity, and a 7.11% increase in crop yield. In contrast, higher doses reduced microbial stability and negatively affected soil health.
Black soils are among the world’s most productive agricultural lands, but decades of intensive farming have led to severe degradation, including organic matter loss and soil acidification. Biochar, a carbon-rich material produced from biomass pyrolysis, has shown promise in restoring soil quality, but its long-term effects on soil microbial communities and dissolved organic matter (DOM) were poorly understood.
Through a six-year experiment, researchers analyzed how different biochar application rates influenced DOM composition and soil bacteria. Using advanced fluorescence spectroscopy and DNA sequencing, they found that the medium application rate promoted the formation of stable humic substances and supported a more complex and resilient microbial network dominated by beneficial bacteria such as Proteobacteria and Acidobacteria .
“These microbes play key roles in breaking down organic matter and releasing nutrients for crops,” said Dr. Lilong Yan, one of the corresponding authors. “Biochar helps create a favorable environment for these organisms, leading to healthier soil and better yields.”
However, excessive biochar (47.25 t ha⁻¹) disrupted microbial balance and reduced diversity, underscoring the importance of calibrated application.
The study also used structural equation modeling to confirm that biochar indirectly boosts crop yields by altering DOM properties and enriching microbial communities.
“Our findings provide a scientific basis for optimizing biochar use in agriculture,” said Dr. Dan Wei, another senior author. “This is especially relevant for sustaining productivity in degraded soils under climate change.”
The research was supported by the National Key Research and Development Program of China and the Chinese Academy of Sciences.
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Journal Reference: Hu, Y., Li, Y., Liu, K. et al. Improving the stability of black soil microbial communities through long-term application of biochar to optimize the characteristics of DOM components. Biochar 7 , 84 (2025). https://doi.org/10.1007/s42773-025-00473-z
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About Biochar
Biochar is the first journal dedicated exclusively to biochar research, spanning agronomy, environmental science, and materials science. It publishes original studies on biochar production, processing, and applications—such as bioenergy, environmental remediation, soil enhancement, climate mitigation, water treatment, and sustainability analysis. The journal serves as an innovative and professional platform for global researchers to share advances in this rapidly expanding field.
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Improving the stability of black soil microbial communities through long-term application of biochar to optimize the characteristics of DOM components
23-Jun-2025