Manuscript published online Monday June 22
“ SLC11A2 withholds divalent metals from Salmonella in the gut epithelium ”
Media Contacts:
Janet Franz | 802-238-8182 | Janet.L.Franz@med.uvm.edu
Stephanie Knific | 281-744-4096 | Stephanie.Knific@med.uvm.edu
BURLINGTON, VERMONT – Salmonella , an infection that causes diarrhea, fever, and abdominal pain, is the most common form of bacterial food poisoning in the U.S., sickening more than a million people each year. Although most healthy people recover without medical treatment, Salmonella infection can spread throughout the body in young children, the elderly and immuncompromised individuals and become a life-threatening infection. A new discovery sheds light on how the human body controls Salmonella infections and open pathways for potential treatments for Salmonella and other food-borne infections.
Research at the Robert Larner, M.D. College of Medicine at the University of Vermont has revealed details about the fight for essential nutrients between Salmonella bacteria and the host during an infection. New evidence discovered by principal investigator Leigh Knodler, Ph.D., Professor of Microbiology and Molecular Genetics, and colleagues demonstrates that specialized intestinal cells control the ability of Salmonella to grow by restricting their access to essential metals, such as iron and manganese.
Epithelial cells lining the intestine form a physical barrier to protect against gut microbes from entering the bloodstream. But some harmful bacteria, such as Salmonella , can breach this barrier and live inside these intestinal cells. In a study supported by a two-year R21 award from the National Institute of Allergy and Infectious Diseases (NIAID) published this week in The Proceedings of the National Academy of Sciences , a peer-reviewed journal of the National Academy of Sciences (NAS), Knodler and colleagues found that intestinal epithelial cells pump iron and manganese away from intracellular Salmonella to restrict their growth in the intestine. This means that if pathogenic bacteria breach the intestinal barrier, then the host has a back-up means of defense..
Using specialized fluorescent sensors of metal ion availability, Knodler and colleagues traced where metal restriction occurs in the gut during an infection and how the human cells use a specialized system (a metal transporter) to withhold these trace metals. The findings highlight a new dimension of host–pathogen interactions and suggest that manipulating metal transport pathways could strengthen the body’s natural defenses. This new knowledge may lead to more novel treatment or diagnostic options for Salmonella and other diarrheal and food-borne illnesses.
“All forms of life, from bacteria to mammals, need essential trace metals. During an infection, there is a fierce biological tug‑of‑war between the human body and microbes for these nutrients, and the outcome can determine the severity of disease,” said Knodler. “Our research shows that intestinal epithelial cells use a metal transporter to starve Salmonella of iron and manganese, and limit bacterial growth. These transporters are potential drug targets for infectious and other human diseases, and our study lays the groundwork for understanding where and how they act in the body.”
Next steps for the research include examining additional metal transporters in the gut—of which there are dozens—to determine whether they also contribute to pathogen control and how they collectively shape the landscape of nutritional immunity. Collaborators on the study include researchers at the University of Wisconsin at Madison, Vanderbilt University Medical Center and John Salogiannis, Ph.D., at the Larner College of Medicine.
###
Research like this has contributed to the University of Vermont’s designation by the Carnegie Classification of Institutions of Higher Education as an R1 institution, placing it in the top tier of research universities in the U. S.
About the Larner College of Medicine at the University of Vermont
Founded in 1822, the Robert Larner, M.D. College of Medicine at the University of Vermont is the seventh-oldest medical school in the nation. The college is dedicated to developing exceptional physicians and scientists by offering innovative curriculum design, state-of-the-art research facilities, and clinical partnerships with leading health care institutions. The college’s commitment to excellence has earned national recognition, attracting talented students, trainees, physicians, and researchers from across the country and around the world. With a focus on inclusive excellence, the Larner College of Medicine prides itself on cultivating an environment that uplifts and supports its faculty and student populations while advancing medical education, research, and patient care in Vermont and beyond. uvm.edu/larnermed
Proceedings of the National Academy of Sciences
Experimental study
Cells
SLC11A2 withholds divalent metals from Salmonella in the gut epithelium
22-Jun-2026
The authors declare no competing interest.