Recently, National Science Review published a paper online of Prof. Fan Xia (Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan) and Prof. Yu Huang (Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan). In this research, droplets’ motion behaviors on the surface were precisely controlled by adjusting the hydrophobic interaction between DNA droplets and lubricant-infused micro-grooves structural surface. Furthermore, based on this strategy, a highly sensitive biosensor system was developed with orthogonal dual-regulation of liquid phase and solid phase.
To realize the target of orthogonal dual-regulation of liquid phase and solid phase, Prof. Fan Xia and Prof. Yu Huang tuned the DNA’s average chain length of droplets with different concentrations of targets by rolling circle amplification (RCA) meanwhile varied droplet’s sliding direction on the lubricant-infused micro-grooves structural surface to adjust the energy barrier from micro-grooves. Based on this strategy, a highly sensitive biosensor system with adjustable dynamic ranges was developed. To testify the utility of this strategy, they employed it in the ultrasensitive detection of ATP, miRNA, Thrombin, and Kanamycin. Moreover, they also found this strategy could be extended to use in some cluster analysis of multi-targets. For example, the discrimination of 5 kinds of DNA including Lamda DNA, Linear DNA, Fish sperm DNA, M13, and PUC119.
Therefore, this orthogonal dual-regulation strategy demonstrated its ability of precise controlling of bio-droplets’ motion behaviors and sensitive detection with adjustable dynamic ranges for various bio-targets. The dual-regulation strategy will provide significant insights for superwettable biosensors, visual inspection, and beyond.
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See the article:
An Orthogonal Dual-Regulation Strategy for Sensitive Bio-sensing Applications
https://doi.org/10.1093/nsr/nwac048
National Science Review