Articles tagged with Double Stranded Dna
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A new study found that recombinant adeno-associated virus (rAAV) capsids contain single-stranded DNA impurities derived from plasmid and host cell DNA. The researchers suggest that the adverse effects of these impurities may differ from those of double-stranded DNA, highlighting the need for further evaluation.
Scientists have captured the first detailed molecular movie of DNA being unzipped at the atomic level, revealing how cells copy their genetic material. The discovery has significant implications for understanding viral and cancer replication.
Researchers have developed a new strategy to protect cancer patients from radiation-induced DNA damage using a protein from tardigrades. The approach makes use of messenger RNA encoding the protein, which is delivered to patient tissues before radiation treatment. This reduces double-stranded DNA breaks by 50% in mouse models.
Researchers are investigating the impact of virus infections on honeybee health, including flight performance and heat shock stress response. The team is also exploring potential supplements to boost immune strength and reduce virus infections in honeybees.
A new study reveals that anti-defense genes near the DNA entry point enable plasmids to overcome CRISPR system, promoting genetic transfer between bacteria. This discovery could pave the way for developing tools to address antibiotic resistance and genetic manipulation methods.
Researchers have discovered a major setback in the use of AZD7648 to promote precise gene editing, which causes massive genetic changes and genome instability. Despite this, scientists remain optimistic about advancing CRISPR-Cas technology to treat diseases.
The special issue explores challenges and opportunities in managing synthetic genomics risks, introducing a common global baseline for nucleic acid synthesis screening. Review articles provide insights into enhancing gene synthesis security and biosecurity practices of synthetic DNA providers.
Researchers developed a new technique called HiDEF-seq to detect early molecular changes in DNA code that precede mutations. The study found higher numbers of single-strand DNA changes in healthy cells from people with genetic syndromes linked to cancer, suggesting a link between these changes and the development of cancer.
Researchers question whether micronuclei activate the cGAS-STING pathway, a key innate immune response to foreign nucleic acids. The study found that MN more commonly recognizes DNA during cell division without triggering STING activation.
Researchers have developed a working nanoscale electromotor powered by hydrodynamic flow through a nanopore. This innovation uses DNA origami to create a turbine with precise control over rotational speed and direction. The tiny motor has potential applications in molecular factories, medical probes, and soft propulsion systems.
Researchers have found that a specific pathway, cGAS-STING, is unleashed to prevent cancer formation by detecting DNA damage within cells. The discovery reveals the 'key' to unleashing this pathway, which can potentially reactivate it to treat and prevent breast cancer development.
Researchers at UNC School of Medicine have pieced together the lesser-known DNA repair pathway, polymerase theta-mediated end joining (TMEJ), which is upregulated in patients with hereditary breast cancer, ovarian cancer, and prostate cancer. The discovery could lead to new therapies for cancer by targeting this pathway.
A new study reveals that autophagy plays a crucial role in the gradual loss of DNA content in diploid Saccharomyces cerevisiae cells undergoing chronological aging. The researchers found that only diploids survived, and autophagy induction was responsible for the DNA loss.
Researchers found that DNA damage accumulates in arteries with aging and contributes to impaired vascular function. In mice lacking or heterozygous for the double-strand DNA break repair protein ATM kinase, aging accelerated vascular dysfunction, including increased arterial stiffness and oxidative stress.
Chung-Ang University researchers create an electrochemical DNA biosensor that detects HPV-16 and HPV-18 with high specificity, facilitating early diagnosis of cervical cancer. The sensor uses a graphitic nano-onion/MoS2 nanosheet composite to enhance conductivity.
A new gene-editing technique combines peptide nucleic acids and prokaryotic Argonautes to introduce targeted breaks in the genome. The approach, called PNP editing, offers advantages over CRISPR-based methods, including improved specificity and targeting.
Researchers developed a new strategy for T-cell-based immunotherapy using aptamers, which directly activates immune cells against cancer cells without genetic modifications. The innovative regulatory circuit establishes an artificial interaction between T cells and cancer cells.
Researchers used DNA-PAINT to study base-stacking interactions in DNA strands, finding that adding one more interaction increases stability by up to 250 times. This information allowed them to design a highly efficient three-armed DNA nanostructure with potential biomedical applications.
Gang Bao's lab receives a 4-year, $2.6 million grant from the National Institutes of Health to investigate the safety and efficacy of using gene editing treatments like CRISPR-Cas9 to treat sickle cell disease. The team aims to understand the mechanisms behind large gene modifications and their biological consequences.
Researchers have discovered how MCV initiates DNA replication in host cells, allowing the virus to make hundreds of new copies of itself. This process is different from normal cellular DNA replication and can lead to cancer if not controlled.
Researchers at Weill Cornell Medicine have discovered a DNA molecule that folds into a four-way junction structure, allowing it to mimic the activity of green fluorescent protein (GFP). This breakthrough could lead to the development of new DNA-based fluorescent tags for rapid-diagnostic tests and various scientific applications.
Researchers have developed a new method to manipulate the shape of double-stranded DNA, known as triplex origami, which can create compacted structures with unique properties. This breakthrough has implications for gene therapy, nanoscale materials engineering, and our understanding of biological processes.
Researchers study DNA minicircles using hydrodynamic measurements to understand their behavior under twisting, revealing unique shapes and compactness. The investigation combines theoretical approaches with experimental methods to elucidate dynamic hydroelastic effects in DNA.
A study by Tokyo Institute of Technology mapped how singlet oxygen molecules travel along DNA strands, shedding light on their propagation and oxidation patterns. The research could lead to more efficient and selective photosensitizer agents for targeted photodynamic therapy, a promising cancer treatment.
The new PASTE tool combines precise targeting of CRISPR-Cas9 with integrases to insert large chunks of DNA into the genome without inducing double-stranded breaks. This approach holds promise for treating diseases with multiple mutations, such as cystic fibrosis, with high efficiency and minimal unwanted effects.
Researchers at Gladstone Institutes and UCSF have developed a new approach to introduce long DNA sequences into cells with remarkable efficiency. The technology, which uses single-stranded DNA templates, overcomes the limitations of traditional viral vectors and has the potential to make cell therapies faster, better, and less expensive.
Researchers have modelled a key mechanism by which DNA replicates, revealing details about how helicases wrangle DNA during replication. The simulations showed each step of translocation can travel more than 12 nucleotides along the backbone, pinpointing interactions involved in long-distance movement.
Researchers at UNSW Medicine & Health unveil a simple mathematical model predicting DNA hybridization rates based on nucleating interactions. The discovery has the potential to improve understanding of biological systems and refine nanotechnologies.
A new study explores the characteristics of 36 basic variants of the Holliday junction, a fundamental building block used in DNA nanoforms. The results show that sequences forming the four protruding arms of the junction can enhance or hinder crystallization processes.
Researchers developed a simple physical model to explain DNA deformations caused by ions and temperature changes. The model reveals that salt-induced twist changes are driven by electrostatic interactions, while temperature-induced changes are related to DNA diameter variation. These findings provide new insights into the molecular mec...
Researchers at Arizona State University have designed and constructed artificial membrane channels using DNA, allowing selective transport of ions, proteins, and cargo. The channels can be opened and closed with a lock and key mechanism, enabling diverse scientific domains such as biosensing and drug delivery applications.
Scientists have successfully developed a gene-editing platform called TALED that can perform A-to-G base conversion in mitochondria, the final missing piece of the puzzle in gene-editing technology. This breakthrough has significant implications for treating previously incurable genetic diseases caused by mutations in mitochondrial DNA.
Researchers at Hokkaido University have developed a tuneable, elastic and temperature-sensitive gel by using complementary DNA strands to connect star-shaped polymer molecules together. The gel exhibits predictable behavior, self-healing properties and durability suitable for medical and engineering applications.
Researchers at Arizona State University have developed a new microscopy method that can track 100 single molecules simultaneously in three dimensions. The technique uses surface plasmon resonance (SPR) technology to precisely image molecular binding events and study their dynamic activities in real time.
A team of scientists from Incheon National University developed a programmable DNA-based microfluidic chip that can perform complex mathematical calculations, such as Boolean logic operations. The chip uses a motor-operated valve system to execute a series of reactions in rapid and convenient manner.
Researchers discover that cytosolic dsDNA from mitochondria can cause cell death and neurodegeneration in PD patients. A new approach using DNAse II may help counteract this effect, potentially leading to a breakthrough in treating Parkinson's Disease.
Scientists discovered the molecular motor that packages genetic material into double-stranded DNA viruses. The advance provides insight into a critical step in the reproduction cycle of viruses such as pox- herpes- and adeno-viruses, which could inspire researchers creating microscopic machines.
Researchers use a scanning tunneling microscope to study DNA hybridization, monitoring changes in electronic properties of single molecules. They discovered plateaus in current traces indicating the formation of double-stranded DNA, providing new insights into chemical reactions and potential applications for DNA-based diagnoses.
Researchers at Harvard's Wyss Institute develop CRISPR-responsive smart materials that can release bound cargo, change structures, or regulate electric circuits. These materials have potential for novel theranostic strategies, point-of-care diagnostics, and regional monitoring of epidemic outbreaks.
St. Jude Children's Research Hospital scientists have determined the structure of the minichromosome maintenance complex, a ring-shaped enzyme that plays a central role in DNA replication. The research proposes a rotary mechanism to initiate DNA replication and may help solve one of biology's greatest mysteries.
Researchers at Carnegie Mellon University developed a synthetic molecule that can recognize and bind to double-stranded DNA or RNA under normal physiological conditions. The Janus gamma PNAs have an extraordinarily high binding energy and can be designed to target genomic DNA for gene editing and transcriptional regulation.
Researchers at IBS discover sequence-dependent information influences liquid-liquid phase separation. Single-stranded DNA forms droplets easily, while double-stranded DNA requires specific conditions due to its rigid structure.
A new study by Osaka University provides crucial evidence for the pathophysiology of systemic lupus erythematosus (SLE), a chronic autoimmune disease. The research highlights the importance of type I interferon in SLE development and suggests that targeting this protein may lead to more effective treatments.
Researchers have discovered a way to control the spin current in double-stranded DNA molecules using temperature gradients. They found that the inherent chirality feature in dsDNA enables spin selection and can act as a filter for spin transport.
Researchers developed the DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) system, allowing quick detection of diseases such as HPV using Cas12a. The system involves adding reagents in one reaction and uses isothermal amplification to boost target DNA cuts, resulting in a fluorescent readout.
Cells use programmed fork arrest to halt DNA replication at terminator sites, controlling life span and preserving genome stability. The process involves proteins working together to calibrate fork movement, preventing constant machinery operation.
Researchers found that anti-DNA antibodies preferentially bind to damaged double-stranded DNA (dsDNA) over native DNA, contributing to the pathogenesis of autoimmune diseases. This study provides mechanistic insight into the formation and properties of pathogenic anti-DNA antibodies.
A team led by Berkeley Lab scientist Gang Ren captured the first 3-D images of individual double-helix DNA segments attached to gold nanoparticles. The images reveal the flexible structure of the DNA segments, which could aid in building molecular devices for drug delivery, biological research, and electronic devices.
At different hydration levels, researchers found that water contributes to subpicosecond structure fluctuations and broadens vibrational transitions in DNA. The study also reveals a pronounced coupling of backbone modes and an energy transfer between them.
A new method for making RNAs has been developed by researchers at the University of Texas Health Science Center at San Antonio, allowing for increased chemical diversity and efficiency. This breakthrough could accelerate the development of diagnostics and therapeutics using RNAs.
Researchers at Rockefeller University developed the first model system to understand the DNA 'replication fork' process in eukaryotic cells. This breakthrough enables scientists to study the molecular tools involved in cell division and may have significant implications for human disease research, particularly cancer.
Researchers developed a simple mechanical model to effectively explain DNA's double-stranded structure and elasticity at the nanoscale. The model shows how extreme conditions can cause DNA conformational changes, and its extension is used to study various phenomena such as sequence heterogeneity and protein-DNA interaction.
Researchers used a coupled discrete wormlike chain-Ising model to simulate DNA stretching and confirm two structural transitions at forces of around 65 pN and 135 pN. Beyond 135 pN, DNA strands peel apart into single-stranded DNAs similar to those obtained through thermal denaturation.
Researchers at National University of Singapore have identified a novel double-stranded DNA structure, dubbed S-DNA, which has sparked a 16-year scientific debate. The team's findings suggest that S-DNA may be a potential binding substrate for DNA intercalators and proteins.
Researchers have successfully built nano spiral staircases with tailored optical material from DNA, modifying light in specific ways. The findings confirm predictions and show promise for developing novel optical lens systems with negative refractive index.
A Berkeley Lab-led team has solved the structure of human FEN1, a key player in DNA replication and repair. The study reveals how FEN1 binds to DNA, opens it by severely bending the template strand, and prepares flaps for joining to new fragments.
Researchers at University of Michigan and University of California, Irvine discover DNA's building blocks 'rock and roll,' forming alternative structures with Hoogsteen base pairs. These fleeting states contain new layers of information stored in the genetic code, shedding light on critical interactions between DNA and proteins.
Researchers at the University of Cincinnati have successfully developed an artificial pore that can transmit double-stranded DNA through a membrane. The engineered channel was created by inserting the modified core of a nanomotor into a lipid membrane, allowing for the movement of single- and double-stranded DNA.
Researchers at the University of Illinois have designed a small molecule that blocks an aberrant pathway associated with myotonic dystrophy type 1. The new compound, Ligand 1, binds tightly to its target, preventing the MBNL protein from binding to RNA and easing symptoms of the disease.
Researchers have invented a new DNA microarray technique that uses electrostatic repulsion to read and evaluate DNA or RNA assays without chemical labeling or sophisticated instrumentation. The technique can be carried out in minutes and has the potential to revolutionize medical diagnostics.