Articles tagged with Nanopore Sequencing
Nottingham researchers demonstrate highly selective DNA sequencing method called Read Until, reducing time needed to analyze biological samples. The technique uses real-time nanopore sequencing and enables analysis of specific DNA strands with pre-determined signatures.
Researchers have developed a complete system to sequence DNA in nanopores electronically at single molecule level with single-base resolution. The system uses a protein nanopore array and polymer-tagged nucleotides to perform single molecule electronic DNA sequencing, enabling real-time and parallel sequencing of multiple DNA molecules.
A new open-source tool called NanoOK provides comprehensive alignment-based quality control and error profile analysis for the MinION platform. The tool is easily extensible through a Java programming interface and handles metagenomic sampling gracefully.
The MinION miniature DNA sequencing device has been evaluated by an international consortium, showing consistent good performance and accuracy across five laboratories. The data is freely available for re-analysis and innovation on F1000Research.
EPFL scientists have developed a method that improves the accuracy of DNA sequencing up to a thousand times by slowing down the process using nanopores and viscous liquids. This breakthrough paves the way for better and cheaper DNA sequencing, enabling scientists to detect mutations and identify different organisms with greater precision.
Researchers have developed a new method for studying protein structure using nanoscopic pores, allowing for the analysis of individual proteins without modification. This technique enables the detection of protein aggregates, which are associated with diseases like Alzheimer's and Parkinson's.
Scientists in Canada and UK successfully sequenced and assembled de novo the full genome of E. coli using Oxford Nanopore's MinION device, providing proof of concept for the technology and its potential to sequence genomes in complex organisms like humans.
A pocket-sized device has shown potential in disease detection, accurately identifying a range of closely-related bacteria and viruses within six hours. The technology relies on protein 'nanopores' to determine DNA sequences, allowing for faster and more accurate identification of pathogens.
Researchers at Berkeley Lab and UC Berkeley have developed a method to produce graphene nanopores with integrated optical antennas, enabling direct optical DNA sequence detection. This approach opens new avenues for simultaneous electrical and optical nanopore DNA sequencing and regulating DNA translocation.
Scientists from Brown University used a specific type of virus to study the interaction between polymer strands like DNA and tiny holes, known as nanopores. The findings may lead to breakthroughs in DNA sequencing and pathogen detection.
Researchers found that long chain DNA with low salt concentration is more conducive to nanopore sequencing, enabling longer reads and potentially reducing costs. This breakthrough has the potential to make gene sequencing more accessible and efficient.
A team of University of Pennsylvania physicists has made progress in the development of a new gene sequencing technique using solid-state nanopores. The researchers successfully differentiated single-stranded DNA molecules containing sequences of a single repeating base, achieving a promising breakthrough in this area.
A review by Northeastern University physicist Meni Wanunu questions the feasibility of nanopore technology for fast and affordable genome sequencing. The main technical hurdles include slow process rates, protein pore limitations, spectroscopic information gaps, and clogging issues.
Recent advances in nanopore sequencing, developed by Stuart Lindsay, demonstrate improved DNA reads and can pinpoint individual bases with greater than 90% accuracy. This technology has the potential to become ubiquitous at a cost below $1000 per genome.
Researchers from Imperial College London have developed technology that could sequence a human genome in mere minutes, potentially unlocking personal susceptibility to diseases. The technology uses nanopores and could lead to fast, inexpensive genome sequencing with numerous benefits for medical tests and DNA profiles.
A new JACS paper demonstrates continuous and controlled translocation of a single-stranded DNA polymer through a protein nanopore using a DNA polymerase enzyme. This achievement advances the development of Strand Sequencing using nanopores, a crucial component of molecular motors.
Researchers at UCSC and Oxford Nanopore Technologies Ltd demonstrate fine control of DNA translocation through a protein nanopore using electronic feedback. This breakthrough advances towards direct, electrical detection and analysis of single molecules for various applications, including DNA sequencing.
The Scripps Research Institute has been awarded a four-year, $5.1 million grant to develop nanopore strand sequencing, a rapid real-time technology that can sequence a person's DNA in 15 minutes with minimal sample preparation time. The goal is to make genome sequencing cost-efficient and routine medical care possible.
Boston University researchers have received a $4.1 million grant to refine their nanoscale, low-cost DNA sequencing method that could lead to individual genome sequencing for less than $1,000. The team's solid state nanopores are uniquely positioned to compete with current DNA sequencing methods for cost, speed and accuracy.
Researchers at Sandia National Laboratories have developed kinked nanopores that can slow down DNA transmission, enabling easier DNA sequencing. The innovation uses self-assembly techniques and atomic-layer deposition to achieve a fivefold slowdown in voltage-driven translocation speeds.
Researchers at the University of Pennsylvania have developed a new, carbon-based nanoscale platform to electrically detect single DNA molecules using graphene nanopores. The platform exhibits high resolution and may provide a way to distinguish among DNA bases, enabling a low-cost, high-throughput DNA sequencing technique.
A new nanopore method for DNA sequencing has been developed by Boston University researchers, enabling ultra-fast and low-cost genetic analysis. The technique uses solid-state nanopores to detect DNA molecules, achieving readout rates of up to 200 bases per second.
The National Human Genome Research Institute has awarded over $20 million in grants to develop innovative DNA sequencing technologies that can sequence a person's genome for $1,000 or less. The goal is to enable routine sequencing of genomes to advance scientific knowledge and healthcare.
Researchers at the University of Illinois have developed a method for sequencing DNA using nanopores, which could lead to a device that reads human genomes quickly and affordably. The technique produces an electrostatic fingerprint that can be used to read the genetic sequence, enabling precise diagnosis and tailored treatment procedures.
Researchers at the University of Illinois have created a semiconductor membrane that can mimic the operation of biological ion channels, with applications in single-molecule detection, protein filtering, and DNA sequencing. The membrane uses electrostatic potentials to regulate charged species and ions, offering a degree of tunability ...
A Northwestern University researcher has explained the nature of the resistive force that determines the speed of DNA as it moves through a nanopore, using classical hydrodynamics. This understanding could help scientists slow down the DNA enough to make it readable and usable for medical and biotechnology applications.
The new nanopore method has the potential to sequence human genomes in a matter of hours at a potentially low cost, reducing the time and expense associated with current methods. The approach uses mathematical calculations and computer modeling to distinguish between DNA bases, enabling faster and more accurate sequencing.
The National Human Genome Research Institute is expanding its efforts to develop faster and cheaper DNA sequencing technologies. The goal is to lower the cost of sequencing a mammalian-sized genome to $100,000 and eventually cut it to $1,000 or less, enabling routine medical care and personalized diagnosis.