Articles tagged with Next Generation Sequencing
Researchers developed a method using next-generation genomic sequencing to isolate infecting organisms after joint replacement surgery. The technique identified pathogens in over 80% of cases that previously went undetected, revolutionizing treatment for patients with joint-replacement infections.
New research suggests that next-generation gene sequencing can rapidly diagnose rare diseases in newborns, providing timely molecular diagnoses and potentially life-saving interventions. The technique has shown diagnostic success rates of up to 40% in a pilot study of 20 infants with complex medical issues.
A study highlights the importance of coupling natural history collections with next-generation sequencing to obtain large molecular data sets for species-rich groups. Large genomic data sets are becoming increasingly obtainable, but the bottleneck shifts to the number of species and individuals that can be included in the study.
A team of researchers from the University of Alberta has shown that betaretrovirus infects patients with primary biliary cirrhosis, a rare autoimmune liver disease. The study used next-generation sequencing to identify viral DNA integration sites in patient genomes, providing evidence of viral infection.
Environmental scientists use next-generation sequencing to track population adaptations to climate change, finding gene variants and altered expression that point to selection for climate-adapted traits. However, funding constraints may hinder the technology's adoption in ecological studies.
Genomic tests using next generation sequencing technologies face access issues due to inadequate evidence on clinical utility. Experts suggest a four-pronged approach to improve access, including robust validation studies, prioritizing research and evidence, and evolving the evidence review process.
Researchers have used next-generation sequencing to rapidly identify pathogens in the United States. A recent discovery of porcine enterovirus G highlights the importance of this technique in detecting viruses that can cause disease. The virus is thought to be benign but raises concerns about other viruses getting through borders.
A study using Sequenta's LymphoSIGHT platform reveals that patients with longer survival have lower numbers of T cell clones that decrease in frequency after treatment. This association holds for both prostate cancer and melanoma patients, suggesting that immune repertoire sequencing could predict clinical benefit of immunotherapies.
ZENBU integrates genome browsers with data analysis and linked expression views to facilitate interactive visualization of next-generation sequencing results. The tool allows for dynamic combination of thousands of experimental datasets, enabling scientists to discover new biological mechanisms.
A new tool called REALPHY reconstructs evolutionary trees from sequencing data without errors and biases. The method is simple enough for biologists to use, generating accurate phylogenies quickly.
Researchers have developed a new approach using long-range PCR and next-generation sequencing to obtain large phylogenomic data sets, increasing accuracy in reconstructing evolutionary history. This method allows for the amplification of larger DNA fragments, enabling the targeting of specific genomic regions.
A new RNA extraction protocol for land plants has been developed, allowing for the extraction of high-quality RNA from a wide variety of plant species and tissue types. This protocol will greatly facilitate RNA-based studies of non-model plant species and enable comparative analyses of transcriptomes across diverse lineages.
New DNA sequencing techniques enable plant scientists to analyze complex genomes, revealing new insights into plant diversity and evolutionary history. Targeted enrichment methods facilitate the study of specific genes and regions, accelerating discovery in crop production, ecosystem health, and understanding plant diversity.
The Wellcome Trust Sanger Institute has sequenced 300 human genomes in six months, producing over 1 trillion letters of genetic code. This data will revolutionize human medical genetics and allow researchers to answer questions previously unthinkable.