Articles tagged with Green Fluorescent Proteins
Comprehensive exploration of living organisms, biological systems, and life processes across all scales from molecules to ecosystems. Encompasses cutting-edge research in biology, genetics, molecular biology, ecology, biochemistry, microbiology, botany, zoology, evolutionary biology, genomics, and biotechnology. Investigates cellular mechanisms, organism development, genetic inheritance, biodiversity conservation, metabolic processes, protein synthesis, DNA sequencing, CRISPR gene editing, stem cell research, and the fundamental principles governing all forms of life on Earth.
Comprehensive medical research, clinical studies, and healthcare sciences focused on disease prevention, diagnosis, and treatment. Encompasses clinical medicine, public health, pharmacology, epidemiology, medical specialties, disease mechanisms, therapeutic interventions, healthcare innovation, precision medicine, telemedicine, medical devices, drug development, clinical trials, patient care, mental health, nutrition science, health policy, and the application of medical science to improve human health, wellbeing, and quality of life across diverse populations.
Comprehensive investigation of human society, behavior, relationships, and social structures through systematic research and analysis. Encompasses psychology, sociology, anthropology, economics, political science, linguistics, education, demography, communications, and social research methodologies. Examines human cognition, social interactions, cultural phenomena, economic systems, political institutions, language and communication, educational processes, population dynamics, and the complex social, cultural, economic, and political forces shaping human societies, communities, and civilizations throughout history and across the contemporary world.
Fundamental study of the non-living natural world, matter, energy, and physical phenomena governing the universe. Encompasses physics, chemistry, earth sciences, atmospheric sciences, oceanography, materials science, and the investigation of physical laws, chemical reactions, geological processes, climate systems, and planetary dynamics. Explores everything from subatomic particles and quantum mechanics to planetary systems and cosmic phenomena, including energy transformations, molecular interactions, elemental properties, weather patterns, tectonic activity, and the fundamental forces and principles underlying the physical nature of reality.
Practical application of scientific knowledge and engineering principles to solve real-world problems and develop innovative technologies. Encompasses all engineering disciplines, technology development, computer science, artificial intelligence, environmental sciences, agriculture, materials applications, energy systems, and industrial innovation. Bridges theoretical research with tangible solutions for infrastructure, manufacturing, computing, communications, transportation, construction, sustainable development, and emerging technologies that advance human capabilities, improve quality of life, and address societal challenges through scientific innovation and technological progress.
Study of the practice, culture, infrastructure, and social dimensions of science itself. Addresses how science is conducted, organized, communicated, and integrated into society. Encompasses research funding mechanisms, scientific publishing systems, peer review processes, academic ethics, science policy, research institutions, scientific collaboration networks, science education, career development, research programs, scientific methods, science communication, and the sociology of scientific discovery. Examines the human, institutional, and cultural aspects of scientific enterprise, knowledge production, and the translation of research into societal benefit.
Comprehensive study of the universe beyond Earth, encompassing celestial objects, cosmic phenomena, and space exploration. Includes astronomy, astrophysics, planetary science, cosmology, space physics, astrobiology, and space technology. Investigates stars, galaxies, planets, moons, asteroids, comets, black holes, nebulae, exoplanets, dark matter, dark energy, cosmic microwave background, stellar evolution, planetary formation, space weather, solar system dynamics, the search for extraterrestrial life, and humanity's efforts to explore, understand, and unlock the mysteries of the cosmos through observation, theory, and space missions.
Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
Researchers at Massachusetts General Hospital developed a living laser device using a single GFP-expressing cell, which can produce hundreds of pulses of laser light. The cellular device refocuses the light and induces emission of laser light at lower energy levels than required for solution-based devices.
Scientists at TUM create customized fluorescent proteins in various colors for future applications by incorporating a genetically encoded non-natural amino acid into widely used natural proteins like GFP. The new bio-molecule exhibits a pseudo-Stokes shift, allowing it to be excited with commercially available black-light lamps.
Scientists have discovered a way to help protein-based drugs enter cells by attaching them to supercharged green fluorescent protein. This approach is up to 100 times more effective than previous methods, allowing proteins to reach their target locations and perform their functions.
Researchers design a new technique called PRIME, which tags proteins with smaller probes allowing them to carry out normal functions. This breakthrough sheds light on previously unseen protein activities, offering new insights into cell biology.
Researchers have identified a new outermost layer of protection on bacterial spores, known as the 'spore crust', which may be a common feature of all spore-forming bacteria. This discovery was made using advanced microscopy techniques and offers new insights into the survival methods of these resilient organisms.
University of California, Berkeley chemists use ultrafast laser pulses to study green fluorescent protein's structural changes during fluorescence. The study reveals the importance of vibrational oscillations in proton transfer reactions, shedding light on how GFP captures and emits light.
A new NIST assay using a 'glow or no glow' technique can detect ricin, a lethal toxin, at low doses and measure its potency with high precision. This standardized sample will aid in the accuracy of detection equipment and decontamination procedures.
Researchers have developed a microscope capable of live imaging at double the resolution of fluorescence microscopy using structured illumination. This advancement will help scientists study cellular behavior and mechanisms important for human disease with enhanced detail.
Researchers from USC and Cambridge have developed a method to track the activity of specific genes in real-time using a specially modified camera and computer vision techniques. This breakthrough has potential applications in various fields, including military, retail, and entertainment.
A new technique allows for the simultaneous tracking of gene expression and movement in Drosophila flies, enabling researchers to study correlations between behavior, gene expression, and aging. The method uses green fluorescent protein (GFP) and specially modified video cameras to track movement and quantify GFP expression in real-time.
A team of scientists has discovered a new green fluorescent protein in a deep-sea creature, which can be used as a marker in living cells and tissues. The protein, named cerFP505, has similar brightness and stability to existing fluorescent proteins, making it an ideal lead structure for super-resolution microscopy.
Researchers used an engineered virus to deliver a glowing protein into newborn brain cells in an animal model of Parkinson's disease, revealing most cells are glial and do not form neurons. Further gene delivery experiments showed no effect on neuron formation but increased the number of oligodendrocytes supporting neurons.
Researchers have designed a new probe to image thousands of protein interactions inside living cells, giving them a tool to untangle signaling pathways. The probes are derived from an enzyme and its peptide substrate, allowing for easy detection of protein interactions.
Researchers at Yerkes National Primate Research Center have developed the first transgenic nonhuman primate model of Huntington's disease, a devastating neurodegenerative disorder. The model is expected to help researchers better understand the disease and develop more effective therapies.
Researchers have created fluorogen activating proteins (FAPs) that enable biologists to monitor biological activities of individual proteins in living cells in real time. The FAPs allow for simple and direct tracking of proteins on the cell surface and within living cells, eliminating cumbersome experimental steps.
Researchers have developed a genetically modified mouse model using the ferritin reporter gene, enabling live cell imaging via magnetic resonance imaging (MRI) without additional substances. This breakthrough overcomes limitations in detecting signal changes in tissues, such as fetal development and the central nervous system.
The latest issue of Cold Spring Harbor Protocols provides guidance on choosing plant tissues, designing test proteins, and setting up microscope equipment to detect green fluorescent protein (GFP) in plants. This information is essential for researchers interested in plant biology and imaging technologies.
Researchers have identified individual, isolated hematopoietic stem cells at the edge of bone marrow using a novel gene expression technique. This breakthrough allows for the study of these rare cells in their natural environment, shedding light on how they maintain their pluripotency and function.
Researchers used a fluorescent marker to predict individual life spans of genetically engineered nematodes, revealing up to four-fold variation in lifespan based on stress levels. The study suggests chance metabolic processes dictate aging rates in genetically identical organisms raised in similar environments.
Researchers developed a new fluorescent imaging technique using FRET (Fluorescence Resonance Energy Transfer) to track glutamate production in individual brain cells. This breakthrough technology will help better understand disease processes and construct new drugs.
Scientists found that a protein inside the rabies virus can enhance the immune response against foreign proteins, enabling the creation of vaccines against other infectious agents. The technique could lead to the development of longer-lasting vaccines against diseases like anthrax.
Researchers have successfully tracked multiple living proteins or cells simultaneously using quantum dots, overcoming limitations of traditional fluorophores. This breakthrough enables real-time observation of protein functions in natural environments, holding promise for medical applications such as understanding disease mechanisms.
Researchers have captured the first pictures of neurofilaments moving along nerve fibers using time-lapse photography, providing a rare glimpse into slow axonal transport. The study suggests that neurofilaments move quickly but infrequently, and may hold clues to understanding nerve malfunction in diseases like Lou Gehrig's.
Scientists have discovered a protein that accumulates at the front end of a cell, enabling it to 'sense' its way to a target. This finding brings researchers closer to understanding chemotaxis, a process crucial for inflammation, disease fighting, and wound healing.
Using genetically manipulated T cells producing a fluorescent jellyfish protein, researchers observed the movement of MEKK2 towards T cell receptors within seconds of antigen binding. This study reveals MEKK2's crucial role in delivering molecular signals to the nucleus and active attachment between immune cells.
Researchers successfully delivered fully functional proteins inside cells using a piece of the AIDS virus, overcoming the bioavailability wall that restricts large molecules. This technique has the potential to treat diseases such as cancer and genetic disorders by inserting working versions of damaged proteins into affected cells.
Scientists have developed a method to deliver large proteins into cells using a molecular passport. The technology allows for lower doses and fewer side effects, making it a promising avenue for therapeutic approaches. This breakthrough could enable the creation of drugs that act only in disease-related cells.
Scientists have developed a technique to produce green fluorescent silk fibers by infecting silkworm larvae with a genetically engineered virus. This approach opens doors for genetic researchers to engineer silk proteins and produces potential economic applications.
Researchers identified Munc13-1 as a novel diacylglycerol target that regulates neurotransmitter release from nerve cells. This study reveals a new mechanism for neurotransmitter release beyond the well-known role of Protein Kinase C.
Researchers use a non-invasive method to visualize genetic activity in living cells, shedding light on chromosome movements and folding. The technique has provided evidence of chromosomal fibers folding and unfolding during natural events.
Scientists at the University of Georgia have discovered a three-dimensional structure of a fluorescent protein found in jellyfish that resembles a glowing green lantern. This discovery could help make it easier to use the protein as a biomarker for studying gene expression and cell lineage.
Researchers have developed a powerful tool to study gene expression by harnessing the glow of green fluorescent protein (GFP) from a Pacific Northwest jellyfish. By altering the protein's structure, scientists can now track two proteins simultaneously and determine if genes are activated at the same time.