Researchers predict a significant increase in projected frequency of multiyear La Niña events under global warming. This is due to enhanced tropical-subtropical interaction and Pacific mean-state warming patterns.
The conference will focus on optimizing patient care in the Latin America region, discussing strategies to improve heart health and address rising cardiovascular disease rates. Key topics include cardiac rehabilitation, heart failure management, and practical approaches for clinicians.
Researchers found that Korean Red Ginseng (KRG) can restore spatial working memory and reduce withdrawal symptoms in mice exposed to alcohol. KRG suppresses the PKA-CREB signaling pathway and inhibits neuroinflammation, suggesting its potential as a novel therapeutic intervention for treating alcohol use disorder.
A team of researchers, including RIT's Joel Kastner, have discovered new evidence of how planets as massive as Jupiter can form through gravitational instability around a young star. The discovery was made using images from the ESO's Very Large Telescope and the Atacama Large Millimeter/submillimeter Array.
Researchers discovered that high blood glucose levels make pancreatic cancer cells more sensitive to chemotherapy, which could lead to improved treatment outcomes. In a mouse model and clinical data, the study found a nearly 40% lower risk of death among patients with elevated glucose levels compared to those with normal levels.
Australian scientists have developed a new method to monitor coral recruitment at a fine scale using underwater macrophotogrammetry, complementing reef-scale photogrammetry to track larval settlement and early survival.
Researchers developed a novel vaccine against SARS-CoV-2 by selecting antigen variants with low binding affinity, resulting in higher neutralizing antibody concentrations. This design strategy may also improve vaccine efficacy for other coronaviruses and herpes viruses.
A team of scientists has developed a broadband frequency-modulated comb based on advanced quantum-dot laser, generating a record 3-dB optical bandwidth of 2.2 THz. This quasi-continuous-wave FM comb does not deliver strong optical pulses, making it favorable for integrated DWDM systems.
Researchers found that honeypot ant honey is effective against Staphylococcus aureus and two species of fungi, including Aspergillus and Cryptococcus. This discovery validates the therapeutic use of ant honey by Indigenous peoples for sore throats and skin infections.
Researchers detail structure and mechanism of short Argonaute protein, sparking hopes for therapeutic applications. The discovery may lead to engineering proteins that can detect threats or trigger cell death in healthy cells.
Researchers believe convection in the mantle was stratified into two distinct layers, isolated from each other, until a phase transition at 660 km depth. This restriction to upper mantle recycling and mixing has implications for our understanding of Earth's primordial composition.
A federally funded study led by University of Cincinnati clinical psychologist Sarah Whitton aims to identify mental health risks and protective factors for sexual minority women. The FAB400 cohort, a racially diverse group of nearly 400 women aged 21-26, will participate in an additional six waves of data collection over 2.5 years.
A study by the CHAMPS network found that 82% of child deaths in low-income countries could have been prevented through targeted interventions. The research used minimally invasive autopsies to investigate causes of death and highlighted the role of infections, with an infectious agent found in 87% of cases.
A recent study published in Applied Physics Letters reveals the dynamics of water molecules in tetra-n-butylammonium bromide semiclathrate hydrate using quasi-elastic neutron scattering. The research found that water molecules rapidly reorient, and their motion is consistent with breaking hydrogen bonds.
A novel symmetric-actuating linear piezoceramic ultrasonic motor (SLPUM) is developed to generate symmetrical, synchronous opposite or backward movements of two movers with the same velocity. The SLPUM doubles the working efficiency of traditional piezoelectric motors and can produce high-precision scissoring effects in microsurgery.
Researchers at NIMS have created a novel adhesive that can be repeatedly bonded and unbonded under controlled conditions. This 'reset-on-demand' technology allows for the recovery and reuse of both the adhesive and substrate, addressing environmental concerns while maintaining strong adhesion.
A recent study analyzing over 2 million UK dogs found that mixed-breed, Labrador retrievers, and Jack Russell terriers are the most common breeds. Meanwhile, flat-faced breeds like French bulldogs and pugs increased health risks, with predictions of a canine healthcare crisis in the UK.
Researchers found that people carrying the APOE e4 gene variant, which increases the risk of Alzheimer's disease, may experience reduced smell detection earlier than those without the gene. The study suggests that testing a person's ability to detect odors could be an early indicator of future memory and thinking problems.
A new study identifies reducing ammonia emissions as a cost-effective measure to reduce fine particulate matter concentrations in Europe. Implementing effective measures in the agricultural sector could achieve significant pollution reductions, avoiding 100,000 premature deaths annually.
A study published in JACC: Heart Failure found that people with heart failure who increase their daily step count experience improvements in health status over a 12-week period. The study suggests that wearable device data, such as step counts, can be clinically significant and inform future clinical trials and care.
A survey of over 20,000 respondents in Japan found that 23% of male and 13% of female respondents were willing to receive the smallpox vaccine for Mpox prevention. Higher vaccination rates were observed among homosexual men compared to their heterosexual counterparts.
A new study suggests that Arctic terns, which fly on the longest migrations of any animal, may be resilient to climate change due to their vast habitats. However, researchers warn that multiple small effects could harm this long-lived species and other species unable to adapt.
A new study enhances radiocarbon dating by creating a precise record of atmospheric shifts over 14,000 calendar years BP. This improvement allows for more accurate dating of sub-fossil samples, enabling researchers to reconstruct past environmental and climate changes during glacial times.
A new study from the University of Missouri found that poverty, low health literacy, cultural beliefs, lack of infrastructure, and political issues hinder diabetes self-management for Haitian migrants. The researchers suggest targeted interventions, such as community gardens and healthcare education through local priests.
Researchers have developed a new bioelectronic technology to map fast electrical signals in plants, revealing how plants respond to touch and stress. The study uses a multi-electrode array technology on the Venus Flytrap, demonstrating that electrical signals originate from sensory hairs.
Researchers at Ben-Gurion University have detected the elusive ethyl radical intermediate in the ethane pyrolysis reaction, a key driver of chemical reactions in the plastics and natural gas industries. This discovery could lead to more efficient production of plastics and other chemicals with reduced byproducts and pollution.
Researchers at University of Houston develop prototype of fully stretchable fabric-based lithium-ion battery, addressing safety concerns and enabling new applications for wearable technology. The innovation uses conductive silver fabric as a platform and current collector, providing stable performance and safer properties.
A newly discovered turtle fossil from the Upper Jurassic period provides valuable information on the species' limb taphonomy and habitat ecology. The fossil, Solnhofia parsonsi, was found in Bavaria, Germany, offering a unique perspective on the ancient environment.
Researchers find that viruses, like bacteriophages, can eavesdrop on bacterial communication and switch from chill mode to kill mode in response to chemical signals. The study reveals tools that control this strategy and demonstrates its abundance, providing new insights into viral behavior.
Researchers found that people with obstructive sleep apnea have a higher risk of cardiovascular disease, with reduced blood oxygen levels being the leading factor. The study suggests that treating sleep apnea could help prevent future cardiovascular outcomes.
The study explores resonance between narrative medicine and traditional Chinese medicine, highlighting similarities in case histories. By integrating ancient wisdom with modern approaches, a more compassionate healthcare system can be developed.
Researchers designed machine learning models to identify children at risk of self-harm, finding that incorporating more data points and diagnostic codes improved detection rates. The models were particularly effective for detecting underrepresented groups, such as Black and Latino youth.
Researchers at the University of São Paulo's Institute of Biosciences successfully developed blastoids from bovine pluripotent stem cells, creating an accessible in vitro model for studying embryogenesis. This breakthrough could lead to improved farm animal reproduction and enable breeding cattle with desirable characteristics.
A study found that taking more daily steps improves symptoms and physical limitations for people with heart failure, with little association seen beyond 5,000 steps. Participants who increased their step counts experienced improved symptom control and physical function.
Researchers analyzed data from 2014-2022 to calculate cumulative incidence rates of mass shooting event types. The study found that social-related mass shootings account for 27% of incidents, while domestic violence-related shootings are underreported, highlighting the need for awareness and support.
A Mount Sinai study published in Nicotine & Tobacco Research found that cigarette smokers are more likely to experience tobacco dependence compared to those who use smokeless tobacco or multiple products. The researchers identified three distinct groups of tobacco users and suggested tailored cessation programs for each group.
Researchers discovered that nine percent of esophageal adenocarcinoma patients harbor cancer-predisposing gene mutations, which may trigger progression from Barrett's esophagus. This finding supports the idea that genetic testing can help risk-stratify EAC patients and potentially accelerate development of new treatments.
Researchers developed a method to reduce platinum-group metal loadings in fuel cells, resulting in competitive performance and better durability. The new process could enable mass production of fuel cell electric vehicles, offering zero emissions and higher energy density.
Researchers found that prolonged droughts have led to Steamboat Geyser's dry spells by clogging pathways for trees to respire and grow. The team matched tree growth periods to regional climate records, revealing a connection between environmental conditions and reduced water supply.
A third of children with a history of social care in England faced school exclusion, according to a new study. The research found that those with special educational needs services or child protection plans were excluded at significantly higher rates than others.
Researchers review the cavity-controlled principle in zeolite-catalyzed MTO reactions, highlighting its impact on product distribution, catalyst deactivation, and diffusion behavior. Control strategies for improved stability and selectivity are also discussed.
Researchers at Technical University of Munich have developed an automated analysis method for identifying and quantifying microplastics. The new process uses Raman microspectroscopy to analyze plastic particles directly, allowing for quick and reliable detection of their size, shape, and composition.
Dual-atom catalysts (DACs) face challenges in converting carbon dioxide into multicarbon products due to C-C coupling difficulties. Researchers at Tohoku University uncovered the causes of this failure through advanced theoretical calculations.
A genetic analysis of individuals buried at Machu Picchu suggests a highly heterogeneous population with diverse ethnic origins. The study finds that many retainers were of Amazonian origin and had mixed ancestries, indicating selective mate choice.
Researchers at Mount Sinai discovered a new family of locally secreted molecules called 'mammokines' that play a crucial role in controlling mammary gland fat abundance. These findings have significant implications for breast health, lactation-related disorders, and metabolic syndromes.
A secondary analysis of a randomized clinical trial found that daily low-dose aspirin increases the risk of intracranial bleeding in healthy older adults, but does not reduce ischemic stroke risk. The study's findings have particular relevance to individuals prone to developing intracranial bleeding after head trauma.
A new approach boosts light absorption in thin silicon photodetectors with photon-trapping structures, increasing the absorption efficiency over a wide band in the NIR spectrum. The findings demonstrate a promising strategy to enhance the performance of Si-based photodetectors for emerging photonics applications.
A study by Ohio State University researchers uses mobile cameras installed on campus buses to count vehicles, detect objects in the road, and track movements. The AI-powered YOLOv4 model provides accurate measurements with high spatial and temporal coverage, which could enhance current traffic systems and improve efficiency.
Researchers at Nagoya University identified a genetic sequence in thale cress crucial for plant reproduction and fertilization. The discovery of the Synergid-specific Activation Element of MYB98 (SaeM) is expected to improve seed production, increase plant yields, and enhance crossbreeding between plant species.
Scientists at the University of Pittsburgh Center for Vaccine Research have identified a protein responsible for lethal liver disease in mice infected with Rift Valley fever virus. Removing this protein, Lrp1, from the liver prevented viral replication and extended mouse lifespan.
Researchers at Massachusetts General Hospital discovered a critical protein, NLRP11, that alerts the body to bacterial infections and initiates an effective immune response. This finding may lead to improved 'humanized' mouse models of infections and diseases involving the immune system.
Researchers propose lab-on-a-chip platforms to continuously monitor stored RBC quality and match units to patient needs. This technology aims to improve transfusion safety and efficacy, particularly for critically ill patients and those on chronic transfusion regimens.
Researchers have identified podoplanin as a key protein driving the spread of mucosal melanoma, a rare and aggressive form of skin cancer. Eliminating this protein could lead to a potential treatment through antibody therapy, offering hope for patients with this devastating disease.
The American Heart Association has funded seven new scientific research projects to explore the connection between migraines, strokes, and cardiovascular disease. The studies will analyze existing datasets using machine learning and aim to create a collaborative group of experts to uncover the causes of increased risk.
A new study reveals that rapid state law changes mean many families will need to drive longer distances to access gender-affirming care for their transgender children. The restrictions have led to 25% of Americans aged 10-17 living more than a day's drive away from a clinic offering such care.
A study using wastewater metrics found accurate assessments of county SARS-CoV-2 incidence and may be the best metric for monitoring virus circulation. Home testing increases and vaccination treatment decrease disease acuity, making wastewater surveillance a reliable option.
Researchers identified 12 rare genetic variants associated with QT interval prolongation, increasing the risk of severe heart rhythm abnormalities and sudden cardiac death. These variants can lead to a more precise clinical approach through improved identification and risk stratification, aligning with precision medicine principles.
A new study uses machine learning to analyze data from DrugAge, a database of chemical compounds modulating lifespan in model organisms. The researchers create four types of datasets to predict whether or not a compound extends the lifespan of C. elegans, using features such as compound-protein interactions and Gene Ontology terms.
Researchers evaluated blood biomarkers for Alzheimer's disease in a multicenter study of 817 Chinese Han samples. The study found that combining APOE genotype with plasma pTau and serum GFAP distinguished Aβ status effectively.
A study published in PLOS ONE found that children's ability to recognize dog emotions, especially aggression, improves with age. Four-year-olds were less able to recognize aggressive dog expressions than six-year-olds and adults. As children grow older and gain experience with dogs, they become better at reading canine facial expressions.
Researchers found that consumers react differently to university-co-developed products and key factors influence these perceptions. Firms can leverage university collaboration to increase product attractiveness, but effective marketing is crucial.
A genetic study has found that a captive-bred population of the Orinoco crocodile, one of the world's most endangered species, possesses sufficient genetic diversity and is suitable for reintroduction efforts. The Colombian government can use this research to initiate new populations in areas where the species has been depleted.
Researchers found that aphid-infested tropical milkweed is less inviting to monarch butterflies, with reduced egg-laying and slower caterpillar development. Aphid-free plants, on the other hand, provide a better chance for monarchs to thrive.
Researchers have identified KIAA0930 as a key factor causing muscle atrophy in cancer cells, which could lead to the development of new anti-cachexia therapies. The study found that KIAA0930 knockdown cells showed increased muscle mass and weight compared to control cells.
A study by I.R.C.C.S. Neuromed found that high consumption of ultra-processed foods significantly increases mortality risk among people with type 2 diabetes. Even those following a Mediterranean diet are at increased risk if ultra-processed foods account for a large share of their diet.
Researchers developed new methods for detecting drug-induced hepatotoxicity using 3D cell cultures and mass spectrometry imaging, identifying biomarkers and effective interventions. A zeolite-based protein corona method was also used to characterize plasma proteins, enabling the identification of low-abundance proteins and their potent...
The Bioaction project leverages bacteria as allies in promoting tissue regeneration, offering a paradigm shift in addressing infections. By developing functional bio-hydrogels, the project aims to accelerate healing and stimulate bone growth, reducing reliance on extended antibiotic therapies.
The National Institutes of Health (NIH) invested approximately $164 billion in project funding prior to first drug approval, with only 0.59% ($0.95/$164 billion) associated with patents providing market exclusivity. This research highlights the limited public interest protections for NIH-funded research on new drugs approved between 20...
A study by Cold Spring Harbor Laboratory researchers found that breast cancer cells trick the immune system using a molecule called MHC-II, allowing them to spread faster and evade treatment. Targeting this molecule may lead to new therapeutics and improve patient outcomes.
Researchers successfully modified the ethylene synthesis pathway in the Japanese luxury melon to increase its shelf-life. The study found that introducing a mutation into the CmACO1 gene reduced ethylene generation, resulting in firmer fruit and longer shelf life.
Research highlights an unexpected disparity in survival for Asian adults compared to white adults in the US, with lower survival rates despite comparable bystander CPR rates. The study, published in the Journal of the American Heart Association, analyzed data from nearly 279,000 out-of-hospital cardiac arrests.
Researchers developed a new type of battery using sheet-like hard carbon and activated carbon with improved structural stability. The materials showed high specific energy and power output, lasting almost no loss in performance after 1,000 charge-discharge cycles.
A new study suggests that replacing traditional protein sources with mealworms in high-fat diets can slow weight gain and improve immune response, inflammation, energy metabolism, and cholesterol profiles. Mealworm protein also beneficially alters the ratio of good to bad cholesterol.
The grant investigates the unknown relationship between myopia and glaucoma by analyzing their effects on the ganglion cell complex. The research aims to identify early markers of glaucoma in myopic eyes, potentially reducing vision loss and improving quality of life.
The American Academy of Neurology has developed an Emerging Issues in Neurology article providing timely guidance on new therapies for early Alzheimer's disease. The guidance explains the eligibility criteria, potential side effects, and limitations of these treatments, which are not a cure for the disease.
The study found that subsurface microbial communities regulate refractory DOM flux to the overlying seawater. Humic-like FDOM components were more strongly correlated with microbes in energy-limiting deep-sea sediments than in eutrophic coastal sediments.
A new study led by UCL researchers found that Caenorhabditis elegans' lifespan is limited by its reproductive self-destruction. The finding raises questions about the validity of insights from this model organism for human ageing advances.
A team of physicists and medical doctors developed a portable Magnetic Particle Imaging (MPI) scanner that can visualize dynamic processes in the human body, such as blood flow, without using radiation. The scanner is designed for real-time endovascular interventions and has the potential to change the field of intervention.
Octapharma's Balfaxar, a prothrombin complex concentrate, has received FDA approval for the urgent reversal of acquired coagulation factor deficiency induced by vitamin K antagonist therapy. The product demonstrated effective hemostasis in 94.6% of patients and was non-inferior to comparator Kcentra.
A study by Spanish researchers outlines the optimal strategy for China to achieve carbon neutrality by 2060, focusing on photovoltaic and wind power. The plan involves deploying 3,844 new commercial-scale plants, expanding ultra-high-voltage transmission systems, and optimizing energy loads.
A new species of armored reptile, Mambachiton, shows that the precursor to dinosaurs and pterosaurs had bony plates on its backbone, indicating that armor was ancestrally present in bird-line archosaurs. The loss and re-evolution of armor is an important aspect of dinosaur evolution.
A recent brain single-cell study found that certain genes are expressed at varying levels in the dorsolateral prefrontal cortex of individuals with PTSD and MDD, suggesting dysregulated pathways. The research could lead to novel diagnostic markers and targeted treatments for these conditions.
A recent study found that increased skin cancer screening is not enough to address racial disparities in melanoma survival rates. Researchers suggest that educating physicians about presentation of melanoma in skin of color and making sure people have access to a dermatologist when they have a suspicious lesion could be more effective.
Researchers warn that unsustainable wildlife trade could alter key ecological processes in biodiverse regions, leading to losses of evolutionary history. The study highlights the need for conservation resources to be directed towards tropical hotspots to prevent extinctions.
A new study by Simon Fraser University researchers highlights the urgent need for immediate methane emission reductions to meet global climate change goals. Delaying efforts will increase the risk of exceeding the 2°C temperature limit, even if CO2 emissions are reduced to net-zero.
An international team of scientists has detected a quasi-periodic oscillation (QPO) signal in the radio band from a Galactic black hole system, revealing features that have never been seen before. The QPO signal may provide the first evidence of activity from a jet launched by a Galactic stellar-mass black hole.
Researchers developed a new method for capturing turbulent flame behavior, providing detailed insights into flame dynamics, ignition processes and combustion efficiency. The high-speed 3D imaging approach can be used to optimize energy production processes and improve fire safety measures.
A first-in-human study demonstrates the efficacy of a novel PRMT5 inhibitor, JNJ-64619178, in treating advanced solid tumors. The treatment showed manageable dose-dependent toxicity and achieved preliminary antitumor activity in patients with various cancer types.
A team of scientists has developed an optically transparent terahertz cavity to manipulate phonon vibrations in semiconducting perovskites. This design allows for on-demand adjustment of ultrafast THz field modulation, benefiting photonic integrated devices and optical communications.
A new study shows that earlier transfer to specialized care is associated with better outcomes for infants with NOWS, including shorter hospital stays and less exposure to medication. The study found that nonpharmacologic interventions, such as swaddling and pacifier use, are critical to clinical outcomes.
A new study finds that climate change poses a significant threat to all listed plant and lichen species, with only a minority of conservation plans taking direct action to address this issue. The researchers urge policymakers to use their findings to inform conservation planning and recovery efforts for these endangered species.
A Washington State University study found that popular health-related videos on TikTok primarily cover sexual health, diet, and exercise topics. Influencer role models and aesthetic details dominate the content, lacking attainable behavior change advice.
An observational study of college students found that young women are more likely to engage in risky behaviors like multitasking and conversation while descending stairs. This is compared to young men who were less likely to engage in these behaviors, suggesting that women may be more distracted while descending stairs.
Researchers found fungi can effectively degrade low-density polyethylene (LDPE) plastic without wood present. In the absence of wood, fungal biodeterioration of LDPE increased, suggesting a potential natural method for plastic waste management.
Researchers found that CAR NK cells lose metabolic fitness when competing with tumor cells for nutrients, leading to resistance. Engineering cells with IL-15 enhances metabolic fitness and provides a longer-lasting anti-tumor response.
Researchers developed an atactic C1 polymer with a high melting point by incorporating amide-linkages into its side-chain. The dense-packing of its side chains enhances hydrogen-bonding interactions, resulting in a higher melting point of up to 130°C.
Researchers have uncovered a novel mechanism of cell death regulation, shedding light on its significance during conditions such as SARS-CoV infection and skin injury. The study reveals that the cleavage of cFLIP restrains cell death during viral infection and tissue injury, favoring tissue repair.
Under warming temperatures, lizards may skip over potential mates to quickly choose a partner. Researchers found this behavior in a social lizard species due to environmental changes.
The BMJ investigation found that pharmaceutical companies contributed £7.5 million to royal colleges between 2015-2022, mainly for events and grants. Critics argue that voluntary industry transparency initiatives do not go far enough and do not guarantee full disclosure.
A new study found that individuals vaccinated with the newer pertussis vaccine show similar immune responses to antigens present and absent from the vaccine, suggesting that asymptomatic infections drive T cell response. This could lead to the spread of the bacteria to vulnerable populations.
A UCLA Health study found that Kundalini yoga increased activity in a region of the brain impacted by stress and associated with memory decline. The study suggests that this type of yoga, which combines movement and meditation, may be beneficial for older women at risk for Alzheimer's disease.
Researchers found no directional change in Arctic spring timing over the past 25 years, instead observing extreme year-to-year variation due to climate variability. This shift is attributed to fluctuating temperatures and snow cover, pushing species to their limits.
Research reveals a significant cross-seasonal connection between Arctic sea ice and Eurasian summertime temperature fluctuations. The study found that changes in Arctic sea ice influence atmospheric waves over the North Atlantic–Barents-Kara Sea–Central Asia regions, leading to corresponding changes in temperature fluctuations.
Researchers at California Polytechnic State University have developed a novel method for storing biological materials such as RNA and proteins in a solid-state, resembling a pill or tablet that dissolves in water for on-demand use. This innovation overcomes current limitations in storage and handling, making it easier to access these m...
Researchers have confirmed that honeypot ant honey possesses unique antimicrobial characteristics, effective against Staphylococcus aureus and two species of fungi. The study marks the first time ant honey has been investigated for its medicinal properties.
The article explores how export diversification and ICT shape employment patterns, with research highlighting the importance of diversified trade baskets to minimize economic uncertainties. Tailored trade policies can unlock employment opportunities for both high-skilled and low-skilled workers, fostering sustainable growth.
A new study published in Interface found that casein, a protein found in cow's milk, significantly improved wound healing in rats compared to control groups. The promising results suggest that casein could replace expensive materials like silver in wound dressings.
A new study using ancient DNA found that workers buried more than 500 years ago came from different parts of the Inca Empire, including Amazonia. The analysis supports historical documentation and archaeological studies, providing insights into the genetic histories and lifeways of Machu Picchu's occupants.
Research found that very light exercise can improve prefrontal executive function, with pupil dilation during exercise indicating subsequent improvement. Pupil-linked neural activity was also shown to activate the brain's noradrenergic arousal system.
The study developed a new synthetic method for homoHPHACs and synthesized a series of compounds with electron-donating to electron-accepting substituents. Substituent effects on structural, optical, redox, and antiaromatic properties were demonstrated, with electron-accepting substituents showing strongest anti-aromatic and aromatic pr...
A study by researchers at the University of Texas at Austin found that institutional investors demand disclosure of climate risk due to its impact on a company's health and performance. The survey of 439 institutional investors showed that mandatory disclosure has an effect on investors' decisions.
Researchers reconstruct two massive family trees from ancient DNA data, shedding light on the lives of a prehistoric European Neolithic community. The study reveals a strong patrilineal pattern, large family sizes, and a potentially fluid exchange network comprising many groups.